Cryopreservation of chicken primordial germ cells by vitrification and slow freezing: A comparative study

Tonus, Céline; Connan, Delphine; Waroux, Olivier; Vandenhove, Benoît; Wayet, Jérôme; Gillet, Laurent; Desmecht, Daniël; Antoine, Nadine; Ectors, Fabien; Grobet, Luc

doi:10.1016/j.theriogenology.2016.09.022

Cited by 13 publications

(11 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…4 . Different from poultry spermatozoa, the BCs and PGCs show good tolerance to both slow freezing and vitrification [ 92 ], which may be attributed to their similarity to somatic cells in structure and cryobiology. The expand in culture, survivability of injected recipient embryos, percentage of germline chimeras, and rate of donor-derived gametes from chimeras are the more challenging technique obstacle for stem cells as genetic materials for cryobanks.…”

Section: Stem Cellsmentioning

confidence: 99%

Poultry genetic heritage cryopreservation and reconstruction: advancement and future challenges

Sun

Zong

et al. 2022

J Animal Sci Biotechnol

View full text Add to dashboard Cite

Poultry genetics resources, including commercial selected lines, indigenous breeds, and experimental lines, are now being irreversibly lost at an alarming rate due to multiple reasons, which further threats the future livelihood and academic purpose. Collections of germplasm may reduce the risk of catastrophic loss of genetic diversity by guaranteeing that a pool of genetic variability is available to ensure the reintroduction and replenishment of the genetic stocks. The setting up of biobanks for poultry is challenging because the high sensitiveness of spermatozoa to freezing–thawing process, inability to cryopreserve the egg or embryo, coupled with the females being heterogametic sex. The progress in cryobiology and biotechnologies have made possible the extension of the range of germplasm for poultry species available in cryobanks, including semen, primordial germ cells, somatic cells and gonads. In this review, we introduce the state-of-the-art technologies for avian genetic resource conservation and breed reconstruction, and discuss the potential challenges for future study and further extending of these technologies to ongoing and future conservation efforts.

show abstract

Section: Stem Cellsmentioning

confidence: 99%

Poultry genetic heritage cryopreservation and reconstruction: advancement and future challenges

Sun

Zong

et al. 2022

J Animal Sci Biotechnol

View full text Add to dashboard Cite

show abstract

“…Indeed, bird PGCs can be reproduced in culture and conserved without irreversible alteration of their biological properties (van der Lavoir et al, 2006;Nandi et al, 2016;Tonus et al, 2016). Two main methods are commonly used for cryopreservation of PGCs in birds: slow freezing (SLF) and ultrafast freezing (Vitrif) (Tonus et al, 2017). These cells can be used to repair gonadal tissues with germ cells of the donor line.…”

Section: Semen Preservationmentioning

confidence: 99%

The current state of the problem of in vitrogene pool preservation in poultry

2020

View full text Add to dashboard Cite

This review presents the current progress in and approaches to in vitroconservation of reproductive cells of animals, including birds, such as cryopreservation and freeze-drying, as well as epigenetic conditions for re storing viable spermatozoa and female gametes after conservation. Cryopreservation is an effective way to preserve reproductive cells of various species of animals and birds. In vitrogene pool conservation is aimed primarily to the restoration of extinct breeds and populations and to the support of genetic diversity in populations prone to genetic drift. It is the combination of ex situ in vivoand ex situ in vitromethods that can form the basic principles of the strategy of animal genetic diversity preservation. Also, use of cryopreserved semen allows faster breeding in industrial poultry farming. Despite numerous advances in semen cryobiology, new methods that can more efficiently restore semen fertility after cryopreservation are being sought. The mechanisms underlying the effect of cryopreservation on the semen parameters of cocks are insufficiently understood. The review reflects the results of recent research in the field of cryopreservation of female and male germ cells, embryonic cells, the search for new ways in the field of genetic diversity in vitro (the development of new cryoprotective media and new conservation technologies: freeze-drying). Molecular aspects of cryopreservation and the mechanisms of cryopreservation influence on the epigenetic state of cells are highlighted. Data on the results of studies in the field of male reproductive cell lyophilization are presented. The freeze-drying of reproductive cells, as a technology for cheaper access to the genetic material of wild and domestic animals, compared to cryopreservation, attracts the attention of scientists in Japan, Israel, Egypt, Spain, and France. There is growing interest in the use of lyophilized semen in genetic engineering technologies. Methods of freeze-drying are developed taking into account the species of birds. Organizational and legal ways of solving the problems of in vitroconservation of genetic resources of farm animals, including birds, are proposed.

show abstract

“…At present, the main storage method is cryopreservation which is a technique for the storage of cells, tissues, or other materials at low temperature. 3,4 Generally, the living cells are cooled to low temperature by special methods. The metabolism in the cells is inhibited by the low temperature so that the cells can be stored for a long time.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Cells are a necessary part of cell transplantation and biological organ research. , In recent years, with the development of biological technology, a large number of high activity cells are paid more attention by researchers and the long-term storage of cells is a huge challenge for us to face. At present, the main storage method is cryopreservation which is a technique for the storage of cells, tissues, or other materials at low temperature. , Generally, the living cells are cooled to low temperature by special methods. The metabolism in the cells is inhibited by the low temperature so that the cells can be stored for a long time.…”

Section: Introductionmentioning

confidence: 99%

Using a Novel Supramolecular Gel Cryopreservation System in Microchannel to Minimize the Cell Injury

Lan

Chen

et al. 2018

Langmuir

View full text Add to dashboard Cite

The storage of living cells is the major challenge for cell research and cell treatment. Here, we introduced a novel supramolecular gel cryopreservation system which was prepared in the microchannel, and the supramolecular gel (BDTC) was self-assembled by gelator Boc- O-dodecyl-l-tyrosine (BDT). This cryopreservation system could obviously minimize the cell injury because the BDTC supramolecular gel had a more compact three-dimensional network structure when the BDT gelator self-assembled in the confined space of microchannel. This compact structure could confine the growth of the ice crystal, reduce the change rate of cell volumes and osmotic shock, decrease the freezing point of the cryopreservation system, and possess better protection capability. Furthermore, the results of functionality assessments showed that the thawed cells could grow and proliferate well and remain the same growth trend of the fresh cells after the RSC96 cells flowed out from the microchannel. This novel method has potential to be used for the cryopreservation of cells, cell therapy, and tissue engineering.

show abstract

Cryopreservation of chicken primordial germ cells by vitrification and slow freezing: A comparative study

Cited by 13 publications

References 52 publications

Poultry genetic heritage cryopreservation and reconstruction: advancement and future challenges

Poultry genetic heritage cryopreservation and reconstruction: advancement and future challenges

The current state of the problem of in vitrogene pool preservation in poultry

Using a Novel Supramolecular Gel Cryopreservation System in Microchannel to Minimize the Cell Injury

Contact Info

Product

Resources

About