Cryopreservation of cord blood after liquid storage

Hubel, Allison; Carlquist, Dale; Clay, Mary; McCullough, Jeffrey

doi:10.1080/14653240310003035

Cited by 19 publications

(24 citation statements)

References 19 publications

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“…The ability to store HSCs for short periods of time is desirable for several reasons and has been studied for a variety of HSC products 34‐41 . Because studies suggesting that holding HSC products for 24 hours should not be detrimental to postthaw recovery, cells frozen no more than 4 hours after collection were compared to cells stored for 24 hours before cryopreservation.…”

Section: Resultsmentioning

confidence: 99%

Long‐term storage of peripheral blood stem cells frozen and stored with a conventional liquid nitrogen technique compared with cells frozen and stored in a mechanical freezer

et al. 2010

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Section: Resultsmentioning

confidence: 99%

Long‐term storage of peripheral blood stem cells frozen and stored with a conventional liquid nitrogen technique compared with cells frozen and stored in a mechanical freezer

et al. 2010

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“…Our own practice at the NHS Cord Blood Bank is to maintain UCB units at these temperatures for up to 24 h before processing is initiated [27]. This is because initial studies involving human HPCs demonstrated that UCB storage at both 4°C and room temperature for up to 24 h led to little or no cell loss [29–31], with slightly higher counts for UCB stored at room temperature [29] and so, consensus is that there is no significant effect on TNC and CD34 + cell viability and hematopoietic CFU when UCB is stored at room temperature for 24 h. However, storage conditions for longer times are conflicting. Recent studies [32] suggest that, with the exception of viable CD34 + cells, there is a significant decrease in TNC, MNC and CD45 + cell viability between 72 and 96 h and, with the exception of viable CD34 + cells and CFU-GM, the reductions are significantly higher with room temperature as opposed to 4°C storage pre-processing.…”

Section: Discussionmentioning

confidence: 99%

Effects of obstetric factors and storage temperatures on the yield of endothelial colony forming cells from umbilical cord blood

et al. 2011

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As umbilical cord blood (UCB) is a rich source of endothelial colony-forming cells (ECFC), our aim was twofold: (1) to examine potential obstetric selection criteria for achieving the highest ECFC yields from UCB units, and (2) to determine whether transient storage temperatures of fresh UCB and cryopreservation of UCB units affected ECFC yield and function. ECFC quality was assessed before and after cryopreservation by their clonogenic proliferative potential. Of the 20 factors examined, placental weight was the only statistically significant obstetric factor that predicted ECFC frequency in UCB. Studies on the effects of storage revealed that transient storage of fresh UCB at 4°C reduced ECFC yield compared with storage at 22°C, while cryopreservation of UCB MNCs significantly reduced ECFC recoveries. To our knowledge, this is the first demonstration that placental weight and temperature of storage prior to processing or culture have significant effects on ECFC frequency in UCB. Our studies further support the evidence that cryopreservation of UCB MNCs compromises ECFC recovery.

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“…[27][28][29] Results of the HLA typing are available within 3 hours, and there is no indication that this brief interval between thawing and transplantation has interfered with engraftment.…”

Section: Assessment Of Red Cell and Rapid Hla Typing Screening Programmentioning

confidence: 99%

Mislabeled units of umbilical cord blood detected by a quality assurance program at the transplantation center

McCullough¹,

McKenna²,

Kadidlo

et al. 2009

Blood

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We instituted procedures to check the identity of cord blood unit provided for transplantation by carrying out ABO and human leukocyte antigen (HLA) typing of the thawed units before transplantation. ABO typing is done using standard techniques. Rapid HLA class I serology is with monoclonal antibody trays (One Lambda Inc) using standard incubations. One mislabeled umbilical cord blood (UCB) unit was detected on the day of intended transplantation by repeat ABO typing of the thawed unit at our transplantation center. Because ABO typing will not detect all labeling errors, the rapid serologic class I HLA typing procedure was done on thawed units just before transplantation for all units without an attached segment. This procedure identified a second mislabeled unit. In a 6-year period, 2 of 871 (0. 2% IntroductionTransplantation of umbilical cord blood (UCB) stem cells for hematopoietic reconstitution is increasing. [1][2][3][4][5] As a result of the promising results with UCB, the US Congress has appropriated funds to substantially increase the number of UCB units collected and stored. UCB banking has now transitioned from research and development 6-10 to a routine operation. [11][12][13][14][15][16][17][18][19][20] The emphasis is now on increasing collections at the lowest possible cost to develop large numbers of banked units and to increase the ethnic diversity of the banked units to make transplantations available to more patients. The operation of UCB banks is becoming standardized, partly the result of the American Association of Blood Banks (AABB) and the Foundation for Accreditation of Cellular Therapy (FACT-NETCORD) standards for the collection, testing, processing, and banking of UCB for transplantation. 21,22 The AABB and FACT-NETCORD standards are similar to current Good Manufacturing Practices and Good Tissue Practices promulgated by the Food and Drug Administration (FDA). 23,24 The FDA has also considered the establishment of a regulatory framework for UCB publishing a recent guidance on licensure. 25 Despite progress with UCB bank standardization, some shortcomings remain. Some banks are not yet accredited; and standards, although vigorous in many ways, leave considerable room for interpretation and variability. 20 Thus, the overall quality of UCB banks throughout the world is not really known. As the largest UCB transplantation center using UCB from multiple banks, we have observed considerable variability in the quality and consistency of UCB that is provided to us for transplantation. 20 Here we report 2 cases of mislabeled UCB from highly reputable UCB banks (1 in the United States and 1 in Europe) and the effectiveness of ABO and rapid human leukocyte antigen (HLA) typing for verifying unit identity for UCB units without a physically attached segment. MethodsAt the University of Minnesota, all somatic cell and tissue products for hematopoietic transplantation and immunotherapy are prepared in the University of Minnesota Medical Center Clinical Cell Therapy Laboratory. Cellular products, such ...

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Cryopreservation of cord blood after liquid storage

Cited by 19 publications

References 19 publications

Long‐term storage of peripheral blood stem cells frozen and stored with a conventional liquid nitrogen technique compared with cells frozen and stored in a mechanical freezer

Long‐term storage of peripheral blood stem cells frozen and stored with a conventional liquid nitrogen technique compared with cells frozen and stored in a mechanical freezer

Effects of obstetric factors and storage temperatures on the yield of endothelial colony forming cells from umbilical cord blood

Mislabeled units of umbilical cord blood detected by a quality assurance program at the transplantation center

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