Cryopreservation of Dormant and Non Dormant Broad-Leaved Trees

Cryopreservation of Robinia pseudoacacia

“…PVS2 (Takagi, 2000); while air-drying or silica-gel is used for desiccation in the encapsulation-dehydration technique (Grout, 1995). The subsequent steps in the latter approach are: preculture of encapsulated tissue (in alginate) in culture medium with an augmented sucrose concentration; dehydration and desiccation of alginate beads and subsequently storing in liquid nitrogen (direct plunging) (Paˆques et al, 1997). This technique too has similar advantages: explants can easily be manipulated (Martinez et al, 1999;Takagi, 2000), large quantities of delicate tissues can be stored (Paul et al, 2000) and non-toxic cryoprotectants (sucrose, glycerol) are applied (Niino and Sakai, 1992;Martinez et al, 1999;Takagi, 2000), thereby avoiding the need for an expensive apparatus, as in controlled freezing (Martinez et al, 1999).…”