Cryopreservation of Indian red jungle fowl ( Gallus gallus murghi ) semen

Rakha, Bushra Allah; Ansari, Muhammad Tayyab; Akhter, Shamim; Hussaın, Iftikhar; Blesbois, Élisabeth

doi:10.1016/j.anireprosci.2016.09.004

Cited by 45 publications

(33 citation statements)

References 48 publications

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“…Allowing prolonged semen storage can also help in the conservation of endangered species and propagate essential animal breeds (Comizzoli, 2015;Thelie et al, 2019). The technique is indeed beneficial; however, its widespread application is challenged by the variability in responses observed from species to species (Rakha et al, 2016) and the stress introduced to cells during processing, storage, and thawing which affects semen quality (Tatone et al, 2010). Previous studies had also reported an observed increase in intracellular reactive oxygen species (ROS) levels, which can be associated with a decrease in sperm quality during cryo-processing and after thawing (Evangelista-Vargas & Santiani, 2017;Kim et al, 2011;Slowinska et al, 2018;Tatone et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Superoxide Dismutase (SOD) Activity in Cryopreserved Semen of Itik Pinas-Khaki (Anas platyrhynchos L.)

Ancuelo

Landicho

Dichoso

et al. 2021

Trop. Anim. Sci. J.

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Cryopreservation induces oxidative stress on sperm due to an increase in the number of reactive oxygen species (ROS), thereby resulting in decreased sperm quality. ROS's destructive potential is normally counteracted in sperm by their innate antioxidant system consisting of enzymes, which include superoxide dismutase (SOD). This study aimed to assess the quality of semen from Itik Pinas-Khaki (IP-Khaki) drakes that were cryopreserved with either 4.5% DMSO or 7.0% glycerol as cryoprotectant through evaluation of total sperm motility (%) and determination of SOD activity (U/mL). Here, semen samples were collected from 12 sexually mature IP-Khaki drakes, an improved egg-type breed of Philippine mallard duck, and processed using modified reported cryopreservation procedure for ducks. Results showed that post-thawing total sperm motility averages of 12.04±5.61% using 4.5% DMSO and 13.99±5.28% using 7.0% glycerol were comparable. Moreover, similar SOD activity levels of 0.39±0.18 U/mL with 4.5% DMSO and 0.33±0.21 U/mL with 7.0% glycerol in 2.00 x 108 IP-Khaki sperm cells were also observed. The observed very low intracellular SOD activity indicates severe damage to sperm cells due to cryopreservation, which resulted in a comparably low total sperm motility with either of the cryoprotectants. Thus, the cryopreservation protocol used is not the optimum for IP-Khaki semen based on the observed considerable decline in sperm motility and very low SOD activity after cryopreservation.

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Section: Introductionmentioning

confidence: 99%

Superoxide Dismutase (SOD) Activity in Cryopreserved Semen of Itik Pinas-Khaki (Anas platyrhynchos L.)

Ancuelo

Landicho

Dichoso

et al. 2021

Trop. Anim. Sci. J.

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“…In a recent study on cryopreservation of Indian Red Jungle Fowl sperm using 11% glycerol as a cryoprotectant, a massive loss was observed in sperm motility, plasma membrane integrity, livability and acrosome integrity (Rakha et al, 2016b). In another experiment, 20% glycerol showed encouraging results of in vitro semen quality tests and fertility (Rakha et al, 2016a(Rakha et al, , 2017d. The fertilising ability of the avian sperm is generally assessed indirectly based on motility, plasma membrane integrity, livability and acrosome integrity in laboratory experiments (Blesbois et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

Cryoprotective Effect of Glycerol Concentrations on Indian Red Jungle Fowl (Gallus Gallus Murghi) Spermatozoa

Rakha

Ansari²,

Akhter

et al. 2018

Avian Biology Research

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Semen cryopreservation protocols for wild avian species need to be optimised in order to achieve optimum post-thaw sperm quality and fertility. The present study was designed to evaluate the cryoprotective effect of different glycerol concentrations (11%, 15% and 20%) on post-thaw quality, recovery rates, absolute livability index and fertility of Indian Red Jungle Fowl (Gallus gallus murghi) semen. Semen was collected from eight mature cocks and cryopreserved for storage at −196 °C. Frozen semen was thawed at 37 °C for 30 s and assessed for motility, plasma membrane integrity, viability and acrosome integrity at 0, 2 and 4 h incubation at 37 °C. Percentages of motility, plasma membrane integrity, viability and acrosome integrity were recorded higher (P<0.05) post-thaw at 0, 2 and 4 h at 37 °C with 20% glycerol compared to 15% and 11% glycerol. Likewise, recovery rates (%) of aforementioned parameters after cryopreservation and absolute livability index were observed highest (P<0.05) with 20% glycerol. By comparing values of R2 after multivariate regression analysis, least negative effects of hours of incubation were observed on semen quality in extenders with 20% glycerol followed by 15% and 11% glycerol. The fertility outcomes (number of fertile eggs, fertility [%], number of hatched chicks, percent hatch and hatchability of fertilised eggs) were recorded higher (P<0.05) with 20% glycerol followed by 15% and 11% glycerol. It is concluded that the concentration of 20% glycerol gives the best cryoprotection for quality and fertility of Indian Red Jungle Fowl semen.

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“…Other methods including primordial germ cells and gonadic tissues technologies are still in progress but may be seen as methods that are complementary to semen cryopreservation (Liptoi et al., 2013; Nakamura, 2016; Sztan et al., 2017; Thélie et al., 2018). Different freezing procedures have been developed to cryopreserve avian semen (Blesbois et al., 2007; Santiago-Moreno et al., 2012; Rakha et al., 2016, 2017a; 2018a,b,c; Thananurak et al., 2016; Chuaychu-noo et al., 2017; Thananurak et al., 2017). However, simplification of the more efficient common methods of chicken sperm cryopreservation is still needed in order to make them available to large environmental and breeding conditions.…”

Section: Introductionmentioning

confidence: 99%

Sucrose increases the quality and fertilizing ability of cryopreserved chicken sperms in contrast to raffinose

Thananurak¹,

Chuaychu-noo²,

Thélie³

et al. 2019

Poultry Science

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Chicken semen conservation is an important tool for programs of genetic diversity management and of endangered breeds’ conservation. However, the method still needs to be improved in order to be applied in a wide variety of environments and breeds. Our objective was to compare the effects of 2 external cryoprotectants saccharides (sucrose and raffinose) on the sperm freezability of a Thai local breed, Pradu Hang Dum, in which semen was frozen with a simple freezing method using nitrogen vapors and dimethyl formamide (DMF). Thirty-six males were selected on their motility vigor score for the experiments. In a first experiment, a large range of sucrose and raffinose doses were tested. Semen quality was evaluated after incubation at 5°C or after cryopreservation in straws in the saline Blumberger Hahnen Sperma Verdünner diluent + DMF (6% v/v) with or without sucrose/raffinose. The best targeted doses of sucrose and raffinose were then kept for experiment 2 that was focused on cryopreserved semen. In this experiment, semen quality was measured on frozen-thawed sperm: different objective motility data evaluated by computer-assisted sperm analysis (CASA), membrane integrity, acrosome integrity, mitochondria function evaluated using flow cytometry, lipid peroxide production assessed by the thiobarbituric acid test. Fertility obtained with frozen-thawed semen supplemented or not with sucrose or raffinose was also evaluated after artificial insemination of laying hens. The presence of sucrose at the osmotically inactive dose 1 mmol significantly increased the vigor motility, membrane integrity, acrosome integrity, and mitochondrial functions of frozen-thawed sperm ( P < 0.05), and showed the highest levels of fertility after sperm cryopreservation (91% vs. control 86%, P < 0.001). Raffinose showed negative effects on in vitro semen quality from 1 to 100 mmol. Fertility was also negatively ( P < 0.001) affected by raffinose (fertility rate 66 to 70%). We thus showed in the present study the high success of a simple chicken sperm cryopreservation method with an external cryoprotectant easily available and cheap, the sucrose, used at an osmotically inactive low concentration.

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Cryopreservation of Indian red jungle fowl ( Gallus gallus murghi ) semen

Cited by 45 publications

References 48 publications

Superoxide Dismutase (SOD) Activity in Cryopreserved Semen of Itik Pinas-Khaki (Anas platyrhynchos L.)

Superoxide Dismutase (SOD) Activity in Cryopreserved Semen of Itik Pinas-Khaki (Anas platyrhynchos L.)

Cryoprotective Effect of Glycerol Concentrations on Indian Red Jungle Fowl (Gallus Gallus Murghi) Spermatozoa

Sucrose increases the quality and fertilizing ability of cryopreserved chicken sperms in contrast to raffinose

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