Cryopreservation of macropodid spermatozoa: new insights from the cryomicroscope

Holt, W. V.; Penfold, Linda M.; Johnston, Susan J; Temple-Smith, Peter; Shaw, Jillian M; McCallum, Christopher; Lindemans, W.; Blyde, David

doi:10.1071/rd99076

Cited by 34 publications

(6 citation statements)

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“…Plasma membrane defects may impair the sperm vitality and motility (40). The decreased motility of the preserved spermatozoa was reported to be as a result of the ultrastructural changes taking place during the process (41). The physical and chemical factors to which a sperm is exposed are the main causes of such alterations.…”

Section: Discussionmentioning

confidence: 99%

A Polyphenol-Rich Extract From Entada abyssinica Reduces Oxidative Damage in Cryopreserved Ram Semen

Sobeh

Hassan

Hassan³

et al. 2020

Front. Vet. Sci.

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The Splinter bean, Entada abyssinica, is widely used in folk medicine. In the current work, we profiled the secondary metabolites from E. abyssinica bark extract using LC-MS and investigated its effect on cryopreserved ram semen. Twenty-eight compounds, including tannins and gallic acid derivatives that prevailed in the extract, were tentatively identified. Results showed that the quality of the post-thawed semen showed a significant improvement when the extract was added to the extender at a concentration of 375 μg/mL. The progressive motility and plasma membrane integrity of sperm cells were significantly increased in the post-thawed semen; however, the total antioxidant capacity (TAC) was insignificantly increased. A significant decrease in the concentration of hydrogen peroxide was detected as well. No significant changes were observed in activities of lactate dehydrogenase (LDH), alanine aminotransaminase (ALT), and aspartate transaminase (AST) within the treated samples. Intact sperm percentage was significantly increased, while apoptotic and necrotic sperm percentages were reduced significantly. Molecular docking of some individual components from the extract revealed their potential to interfere with the apoptosis cascade in which Bcl-2 is involved. In conclusion, Entada abyssinica appears to be useful for cryopreservation presumably owing to its polyphenol content that has potent antioxidant capacity scavenging reactive oxygen species (ROS), enhancing the endogenous antioxidant system and inhibiting lipid peroxidation.

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Section: Discussionmentioning

confidence: 99%

A Polyphenol-Rich Extract From Entada abyssinica Reduces Oxidative Damage in Cryopreserved Ram Semen

Sobeh

Hassan

Hassan³

et al. 2020

Front. Vet. Sci.

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“…However, if the postthaw samples are viewed at temperatures below about 20°C, the postthaw motility can be as high as 70%. 38 This effect is caused by a remarkably rapid destabilization of the plasma membrane when the temperature increases above a narrow threshold, typically around 22°C. In this case the action of a high glycerol concentration appears to be two-fold: it apparently protects the spermatozoa during cooling and freezing, but induces extensive damage after thawing.…”

Section: Current Efforts On Comparative Sperm Cryobiology In Mammaliamentioning

confidence: 99%

Biobanking efforts and new advances in male fertility preservation for rare and endangered species

Comizzoli

2015

Asian J Androl

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Understanding and sustaining biodiversity is a multi-disciplinary science that benefits highly from the creation of organized and accessible collections of biomaterials (Genome Resource Banks). Large cryo-collections are invaluable tools for understanding, cataloging, and protecting the genetic diversity of the world's unique animals and plants. Specifically, the systematic collection and preservation of semen from rare species has been developed significantly in recent decades with some biobanks now being actively used for endangered species management and propagation (including the introduction of species such as the black-footed ferret and the giant panda). Innovations emerging from the growing field of male fertility preservation for humans, livestock species, and laboratory animals are also becoming relevant to the protection and the propagation of valuable domestic and wild species. These new approaches extend beyond the “classical” methods associated with sperm freezing to include testicular tissue preservation combined with xenografting or in vitro culture, all of which have potential for rescuing vast amounts of unused germplasm. There also are other options under development that are predicted to have a high impact within the next decade (stem cell technologies, bio-stabilization of sperm cells at ambient temperatures, and the use of genomics tools). However, biobanking efforts and new fertility preservation strategies have to expand the way beyond mammalian species, which will offer knowledge and tools to better manage species that serve as valuable biomedical models or require assistance to reverse endangerment.

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“…A major advance in this area came with the use of the cryomicroscope and fluorescent molecular probes to assess membrane integrity used these techniques to discover that kangaroo sperm frozen in 20% glycerol and a rate of 10 8C/min could maintain a post-thaw motility of 70%, as long as the sperm were not warmed above 20 8C. Warming the sperm above this temperature quickly compromised the integrity of the plasma membrane [47][48][49][50][51].…”

Section: Sperm Freezing and Low Temperature Extensionmentioning

confidence: 99%

Artificial insemination in marsupials

Rodger¹,

Paris²,

Czarny³

et al. 2009

Theriogenology

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Assisted breeding technology (ART), including artificial insemination (AI), has the potential to advance the conservation and welfare of marsupials. Many of the challenges facing AI and ART for marsupials are shared with other wild species. However, the marsupial mode of reproduction and development also poses unique challenges and opportunities. For the vast majority of marsupials, there is a dearth of knowledge regarding basic reproductive biology to guide an AI strategy. For threatened or endangered species, only the most basic reproductive information is available in most cases, if at all. Artificial insemination has been used to produce viable young in two marsupial species, the koala and tammar wallaby. However, in these species the timing of ovulation can be predicted with considerably more confidence than in any other marsupial. In a limited number of other marsupials, such precise timing of ovulation has only been achieved using hormonal treatment leading to conception but not live young. A unique marsupial ART strategy which has been shown to have promise is cross-fostering; the transfer of pouch young of a threatened species to the pouches of foster mothers of a common related species as a means to increase productivity. For the foreseeable future, except for a few highly iconic or well studied species, there is unlikely to be sufficient reproductive information on which to base AI. However, if more generic approaches can be developed; such as ICSI (to generate embryos) and female synchronization (to provide oocyte donors or embryo recipients), then the prospects for broader application of AI/ART to marsupials are promising. #

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Cryopreservation of macropodid spermatozoa: new insights from the cryomicroscope

Cited by 34 publications

References 0 publications

A Polyphenol-Rich Extract From Entada abyssinica Reduces Oxidative Damage in Cryopreserved Ram Semen

A Polyphenol-Rich Extract From Entada abyssinica Reduces Oxidative Damage in Cryopreserved Ram Semen

Biobanking efforts and new advances in male fertility preservation for rare and endangered species

Artificial insemination in marsupials

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