Cryopreservation of Plant Germplasm

Keller, E. R.; Senula, Angelika; Davey, M. R.; Anthony, Paul

doi:10.1002/9780470686522.ch8

Cited by 5 publications

(1 citation statement)

References 28 publications

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“…In case of using in vitro plants as source material, their micropropagation to establish the explant numbers needed for the accession, is also costly. More details of the procedures can be found in relevant laboratory manuals (Keller and Senula 2010;Panis 2008).…”

Section: Cryopreservationmentioning

confidence: 99%

Comparing costs for different conservation strategies of garlic (Allium sativum L.) germplasm in genebanks

Keller

Zanke

Senula

et al. 2012

Genet Resour Crop Evol

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The maintenance of plant genetic resources in living plant collections (genebanks) causes costs due to employment of staff, usage of buildings, equipment and consumables. Since this is especially challenging in vegetatively propagated material, studies were performed for the case of garlic, which is one of the major vegetatively maintained crops in the genebank of IPK Gatersleben. Data were recorded to compare various scenarios of the main strategies field maintenance and cryopreservation. A spreadsheet tool was developed to be used for cost assessment and for drawing conclusions concerning the most effective way of maintenance. Field culture is cheaper in the short term, whereas after a break-even point cryopreservation becomes the more efficient storage method in the long term. This breakeven point depends on the particular scenario, which is determined by various factors such as field and in vitro multiplication rates of various genotypes, presence of bulbils in a part of the genepool, the sample size of the accessions as well as the number of stored accessions in cryopreservation. The comparative discussion is exemplified for a 1-year field rotation versus cryopreservation using either in vitro plantlets or a combination of bulbils and unripe inflorescence bases as organ sources. For the more expensive use of in vitro plants cryopreservation becomes less costly than field culture only after 13 years, whereas this is the case already after 8-9 years when using a combination of bulbils in winter and inflorescence bases in summer.

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Section: Cryopreservationmentioning

confidence: 99%

Comparing costs for different conservation strategies of garlic (Allium sativum L.) germplasm in genebanks

Keller

Zanke

Senula

et al. 2012

Genet Resour Crop Evol

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Cryopreservation of Plant Cells

Keller¹,

Senula²,

Höfer³

et al. 2013

Encyclopedia of Industrial Biotechnology

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Cryopreservation, storage of living tissues in or above liquid nitrogen, is increasingly used to preserve germplasm in the long term. When cooling plant tissue to low temperatures, water may destroy cells by osmotic drying or disruption of organelles by forming ice crystals. The organisms accumulate cryoprotective substances to adapt to freezing. Cryoprotectants trigger glass transition (vitrification), when temperature is cooled down rapidly. In the course of cryopreservation, tissues are imposed by various influences causing oxidative stress: wounding, dehydration, and direct liquid nitrogen effect. Occurring reactive oxygen species may be measured and antioxidant substances may be added. Cell structure alterations, water status, regeneration from cryopreservation injury, and genetic and epigenetic changes, which may occur, were investigated. Various plant tissues were cryopreserved: dedifferentiated cells and tissues; shoot tips; axillary buds and other meristematic plant parts; dormant buds of woods species, seeds, embryos, and embryo axes; and pollen. Several main methods were used: slow freezing, vitrification, and encapsulation. Liquid nitrogen can also be used to eliminate viruses from the organs (cryotherapy). The existence of endogenous or contaminating microorganisms needs special attention. Furthermore, technological considerations and the use of cryopreservation to maintain transgenic or patented material need to be mentioned.

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