Cryopreservation of seeds of Melocactus zehntneri Braun ex Ritter f. and Cereus gounellei Luetzelb ex Schum k. by the vitrification method

This study examined the effects of tocopherol (Vitamin E) and phloroglucinol (1,3,5-trihydroxybenzene) on cryopreserved plantlets of Citrus jambhiri Lush and Citrus Maxima Merr., two important Citrusspecies used in citrus breeding and variety development. After undergoing cryopreservation using conventional desiccation methods, the seeds of these two species were propagated in Murashige and Skoog (MS) medium with various concentrations of tocopherol and phloroglucinol. Plantlets raised from both cryopreserved and non-cryopreserved seeds were multiplied using clonal propagation on MS medium supplemented with the specified two chemicals. The clones were subsequently assessed by a range of procedures, including the quantification of chlorophyll levels, measurement of electrolyte leakage, and evaluation of specific antioxidant enzyme activities, including superoxide dismutase, catalase, and peroxidase. Cryopreserved and non-cryopreserved C. jambhiri and C. maxima clones subjected to 2.0 µM and 15.0 µM tocopherol showed improved membrane stability compared to their control counterparts. Increased photosynthetic efficiency was attained by supplementing 0.3 µM and 4.0 µM phloroglucinol to C. jambhiri and C. maxima, respectively. Both cryopreserved and non-cryopreserved C. jambhiri clones had their maximum antioxidant enzyme activity when cultured in a medium with 2.0 µM tocopherol and 0.3 µM phloroglucinol. In C. maxima, cryopreserved and non-cryopreserved clones had higher antioxidant enzyme activity in medium with 15.0 µM tocopherol and 4.0 µM phloroglucinol. In conclusion, the use of vitamin E and phloroglucinol in cryopreservation can greatly improve the important developmental traits of cryopreserved citrus plantlets.

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Cryopreservation of Lavender Trumpet Tree (Handroanthus impetiginosus) Seeds

Campos,

Pereira,

El Merzougui

et al. 2024

Horticulturae

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In response to the near-threatened status of Handroanthus impetiginosus, primarily due to habitat loss and illegal logging, this study examines how X-ray imaging and cryopreservation impact the seed quality and viability essential for conservation. Seeds initially had a moisture content of 12.3%, reduced to 6.5% through desiccation. X-ray imaging allowed for detailed visualization of internal structures, identifying seeds as normal, abnormal, or dead based on damage and development. Normal seeds consistently germinated and produced healthy seedlings, while those with internal damage or excessive desiccation either resulted in abnormal seedlings or did not germinate. Various cryopreservation treatments were tested, including storage at −80 °C and liquid nitrogen immersion (LN), with and without vitrification solutions (PSV2; PVS3; PSV2 + 1% phloroglucinol; PSV3 + 1% phloroglucinol). Results indicated that immersion in LN without cryoprotectants achieved the highest germination and seedling viability, whereas vitrification solutions, such as PVS2 and PVS3, negatively affected germination. This study demonstrates that X-ray imaging is an effective tool for assessing seed quality and detecting internal damage, while cryopreservation without cryoprotectants is suitable for long-term seed storage. This work highlights the benefits of combining X-ray assessment with optimized cryopreservation techniques to support the conservation of threatened species.

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Cryopreservation of seeds of Melocactus zehntneri Braun ex Ritter f. and Cereus gounellei Luetzelb ex Schum k. by the vitrification method

Cited by 4 publications

References 17 publications

Seed cryopreservation without vitrification (PVS2) induces oxidative stimuli to promote endoreplication in red pitaya seedlings

Seed cryopreservation without vitrification (PVS2) induces oxidative stimuli to promote endoreplication in red pitaya seedlings

Unlocking the Potential of Phloroglucinol and Tocopherol in Improving the Photosynthetic Efficiency, Membrane Stability, and Antioxidant Enzyme Activities of Cryopreserved In Vitro Raised Plantlets of Citrus maxima Merr. And Citrus jambhiri Lush

Cryopreservation of Lavender Trumpet Tree (Handroanthus impetiginosus) Seeds

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