Cryopreservation of semen in shabout (Barbus grypus Heckel, 1843): sperm motility and fertilization rates

Abstract

Summary The effects of three different dosages of Dimethyl‐sulf‐oxide (DMSO) (7, 10, 15%) were investigated on post‐thaw motility of frozen spermatozoa from the shabout (Barbus grypus H., 1843). Three aliquots were diluted in each DMSO treatment at a final proportion of (10% sperm, 83% glucose extender and 7% DMSO), (10% sperm, 80% glucose extender and 10% DMSO) and (10% sperm, 75% glucose extender and 15% DMSO), respectively. Compared to fresh milt, cryopreserved spermatozoa showed a significant decrease in sperm motility. Semen frozen with the extender 1 and 3 containing 7 and 15% DMSO showed a lower percentage of motile cells (36.56 ± 1.14 and 20.89 ± 0.86%) than the extender 2 containing 10% DMSO (41.28 ± 0.83%), and this difference was significant (P < 0.05). Similarly, the extender containing 10% DMSO gave the best fertility rate (36.12 ± 1.57%).

Effect of cryoprotectant on the motility, viability, fertilization, and DNA integrity of naleh fish Barbonymus sp. (Cyprinidae) sperm

“…However, the concentration of DMSO used is species-dependent. For example, the best dilution for R. tawarensis was 5% (Muchlisin et al, 2020), 10% for C. carpio (Irawan et al, 2010), and 10% for B. grypus sperm (Dogu, 2012). This study showed the use of a single concentration across all CPAs.…”

“…DMSO was also suitable for the sperm of depik fish Rasbora tawarensis (Muchlisin et al, 2020), seurukan fish Osteochillus vittatus (Muthmainnah et al, 2018), African catfish Clarias gariepinus (Omitogun et al, 2010), Mugil cephalus (Balamurugan et al, 2019), Barbus grypus (Dogu, 2012), common carp Cyprinus carpio (Irawan et al, 2010), and mahseers Tor tambroides and T. dauronensis (Chew et al, 2010). However, the concentration of DMSO used is species-dependent.…”

Preservation of black sharkminnow,Labeo chrysophekadion(Bleeker, 1849) spermatozoa

“…The sperm concentration (×10 9 sperm mL −1 ) of L. chrysophekadion ranged from 8.80 to 18.20 which was in the range values that have been reported for other cyprinid fish such as in Barbus group, 12.45–18.81 for B. barbus (Alavi, Psenicka, Rodina, Policar & Linhart ), 8.60–25.30 in C. idella (Bozkurt, Ogretmen, Ercin & Yildiz ), 9.80–20.20 for B. sharpeyi (Alavi, Jorfi et al . ), 13.30–34.80 in Vimba vimba (Alavi, Kozak, Hatef, Hamackova & Linhart ), 19.38 ± 2.89 for Barbus grypus (Dogu ) and 16.96–18.81 in C. carpio (Cejko, Krejszeff, Judycka, Sarosiek, Dietrich, Kucharczyk & Kowalski ) (Table ). Differences in spermatozoa concentration among species might be due to the frequency of stripping and spermiation induction (Billard, Cosson, Crim & Suquet ).…”

Inhibitory effect of K+ and Ca2+ concentrations, pH, and osmolality of activation solution on motility of shabut (Barbus grypus Heckel 1843) spermatozoa

“…The aim of B. grypus cultivation is the production of fingerlings for restocking dam lakes and the production of cultured fish broodstocks. Although there is a cryopreservation study (20), there is little information about B. grypus sperm, especially sperm activation, and it has not been previously studied. Although information on the basic reproductive biology of this species is available, the effect of pH and cations on spermatozoa motility is unknown.…”