Cryopreservation of sterlet (Acipenser ruthenus) spermatozoa using different cryoprotectants

Abstract

The present study examined the effectiveness of different cryoprotectan uses for cryopreservation of sterlet (Acipenser ruthenus) sperm. Four different cryoprotectans [dimethylsulfoxide (DMSO), dimethylacetamide (DMA), ethyleneglycol (EG) and methanol (MET)] in concentrations of 5 and 10% in the extender media (30 mM M sucrose, 1 mM M KCl, 25 mM M TrisHCl pH 8.5) were used for the cryopreservation of sperm from five sterlet males. Percentages of motility, velocity, fertilization and hatching rate were measured. The highest post-thawing motility was observed in sperm samples cryopreserved with 10% MET (46 ± 19%), 10% DMA (47 ± 18%) and 10% DMSO (45 ± 7%). Fertilization rate in the control (fresh sperm) was 69 ± 9% and the hatching rate 61 ± 8%. A higher hatching rate after cryopreservation was obtained with 10% MET (32 ± 17%) and 5% DMA (23 ± 3%) when compared to data obtained with 10% DMA (9 ± 5%), 5% MET and 5 and 10% DMSO, wherein the hatching rate was around zero. Our results demonstrate that DMA can be used for cryopreservation of sturgeon spermatozoa.

New extender for cryopreservation of Siberian sturgeon (Acipenser baerii) semen

“…The composition of extenders for the cryopreservation of sturgeons sperm usually consists of 23.4-30.0 mM sucrose and 0.25-1.0 mM KCl buffered by Tris-HCl supplemented with cryoprotectants (5-10% of methanol, ME2SO or DMA) [5,12,16]. Our work indicates that after the reduction of components in the extender to glucose and methanol this procedure can be an alternative cryopreservation method to those previously described for sturgeons.…”

Cryopreservation of semen in shabout (Barbus grypus Heckel, 1843): sperm motility and fertilization rates

“…Boryshpolets et al. () examined the effects of 5 and 10% DMSO on post‐thawing motility in Sterlet semen extender in medium concentrations of dimethylacetamide (DMA), ethyleneglycol (EG) and methanol (MET).…”

“…For the 5, 10 and 15% DMSO level, the post-thaw motility of C. chalcoides spermatozoa should a similar trend as observed in this study (18.7, 35.2, 23.4%, respectively;Lahnsteiner et al, 2000) with the medium DMSO level giving the best results. Boryshpolets et al (2011) examined the effects of 5 and 10% DMSO on postthawing motility in Sterlet semen extender in medium concentrations of dimethylacetamide (DMA), ethyleneglycol (EG) and methanol (MET).…”

Disaccharide combinations and the expression of enolase3 and plasma membrane Ca2+ATPase isoform in sturgeon sperm cryopreservation

“…Of the many disaccharides, sucrose has been widely used in the cryopreservation of sturgeon sperm (Alavi, Cosson, Karami, Amiri, & Akhoundzadeh, ; Billard et al., ; Boryshpolets et al., ; Tsvetkova, Cosson, Linhart, & Billard, ). Trehalose has reported to improve post‐thaw sperm characteristics in mammals such as sheep (Aisen et al., ), mice (Hino, Takabe, Suzuki‐Migishima, & Yokoyama, ), dog (Yildiz, Kaya, Aksoy, & Tekeli, ), boar (Gutierrez‐Perez, Juarez‐Mosqueda Mde, Carvajal, & Ortega, ), cattle (Oh et al., ) and so on.…”