Cryopreservation of tissue-engineered epithelial sheets in trehalose

Chen, Fanfan; Zhang, Wenjie; Wu, Wei; Jin, Yuqing; Chen, Lian; Kretlow, James D.; Gao, Weiyi; Dai, Zhijun; Wang, Jiaming; Zhou, Guangdong; Liu, Wei; Cui, Lei; Cao, Yilin

doi:10.1016/j.biomaterials.2011.07.008

“…Different methods were used for epidermal substitute cryopreservation, such as the use of dimethyl sulfoxide [6,31,32] and trehalose as cryoprotectants [6]. Dimethyl sulfoxide has also been tested on dermal substitutes [33,34].…”

Section: Volume 1 Issue 2 -2017mentioning

confidence: 99%

“…In order to increase efficiency, rapid access to skin substitutes is critical. Therefore, optimized storage methods are needed [6].Cryopreservation is an approach that has been under much investigation recently. The impact of various freezing conditions has been tested on animal and human skins.…”

mentioning

confidence: 99%

Effects of Freezing on Functionality and Physicochemical Properties of A 3D-Human Skin Model

Pouliot¹,

Angers²,

Dubois-Declercq³

et al. 2017

JDC

1

0

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Short AbstractThree-dimensional skin substitutes reconstructed by tissue engineering have strong potential to emulate skin conditions in vivo, although their production necessitates a relatively long period of time. Storage of cryopreserved substitutes among time, using some kind of banking system, would be a conceivable solution to make their utilization more appealing for dermopharmaceutical testing. This study evaluated the effects of freezing at -20 °C over a period of 2 months on the structural and physicochemical properties of skin substitutes produced by tissue engineering. IntroductionLocated at the interface between the human body and the exterior environment, skin is the first line of defense against pathogens, chemicals, and harmful mechanical stimuli [1]. Due to its large surface area and its structural and functional complexity, regenerating a complete human skin represents a big challenge. Over the past years, different human skin substitute models have evolved in order to treat victims of severe burns and chronic cutaneous wounds [2]. Skin substitutes are useful in both applied and fundamental fields of research. They can be grafted as skin replacement in clinical applications or used for various types of skin tests, such as dermopharmacology and toxicology studies [2,3]. Skin substitutes properties must be as close as possible to those of normal skin [4].Over the past decade or so, the use of animals in fundamental and applied research has been subject to social debates, and this has led to the creation of more restrictive legislations, such as the addition of the Seventh Amendment to the European Union's Cosmetic Directive [4]. Utilization of skin substitutes to perform tests allowed for the elimination, or at least reduction, of controversial animal testing. Moreover, as animal skin differs in many ways to human skin, well representative skin substitutes could improve the validity of performed tests [5]. In order to increase efficiency, rapid access to skin substitutes is critical. Therefore, optimized storage methods are needed [6].Cryopreservation is an approach that has been under much investigation recently. The impact of various freezing conditions has been tested on animal and human skins. Among all tests performed, permeability analysis is one of the most commonly used in the literature. This test is based on the fact that skin, despite its crucial involvement as a barrier against exogenous material, remains permeable to most substances. AbstractBackground: Three-dimensional skin substitutes reconstructed by tissue engineering have strong potential to emulate skin conditions in vivo, although their production necessitates a relatively long period of time. Storage of cryopreserved substitutes among time, using some kind of banking system, would be a conceivable solution to make their utilization more appealing for dermo pharmaceutical testing. This study evaluated the effects of freezing at -20 °C over a period of 2 months on the structural and physicochemical properties of skin substitu...

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“…KCs were isolated as previously described. 22 Primary KCs were suspended in KC-serum free medium (Gibco ® ; Life Technologies, Carlsbad, CA, USA) and seeded onto tissue culture plates (BD Pharmingen, Franklin Lakes, NJ, USA) at 3.5 × 10 4 cells/cm 2 in 10 mL of complete medium and incubated at 37°C with 5% CO 2 . Culture medium was changed every other day.…”

Section: Transplantation Of Engineered Epidermis In a Mouse Wound-heamentioning

confidence: 99%

“…Our previous work demonstrated that chitosan-gelatin membranes, which are also slowly degrading materials, could be used for epidermis engineering in animal models as well as in patients. 6,22 Compared with chitosan-gelatin membranes, the current GT/PCL nanofibrous membranes could provide a biomimetic substrate for cell adhesion and proliferation. In addition, the fiber networks create better permeability, which allows for the easy passage of liquid.…”

mentioning

confidence: 99%

Engineering of epidermis skin grafts using electrospun nanofibrous gelatin/polycaprolactone membranes

Duan¹,

Feng²,

Guo³

et al. 2013

IJN

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Skin engineering provides a new strategy for treating a wide variety of skin defects. In particular, electrospun nanofibrous membranes have been used as carriers for epidermis engineering. The aim of this study was to investigate the feasibility of a modified gelatin and polycaprolactone (GT/PCL) electrospun membrane for epidermis engineering. The biocompatibility of the membranes was evaluated by seeding HaCaT cells (human keratinocyte cell line) on the membrane and the mechanical properties of the membranes were determined with and without cells after culture. A cell proliferation assay showing that HaCaT cells attached and proliferated well on the membranes demonstrated that the membranes possess good biocompatibility. Mechanical tests showed that the membranes are strong enough to be handled during transplantation. Further in vivo transplantation studies revealed that epidermises engineered with GT/PCL membranes were able to repair skin defects in the nude mouse. These results demonstrate that GT/PCL electrospun membranes could be suitable scaffolds for skin engineering.

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“…In order to increase efficiency, rapid access to skin substitutes is critical. Therefore, optimized storage methods are needed [6].…”

Section: Introductionmentioning

confidence: 99%

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2017

JDC

0

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Short AbstractThree-dimensional skin substitutes reconstructed by tissue engineering have strong potential to emulate skin conditions in vivo, although their production necessitates a relatively long period of time. Storage of cryopreserved substitutes among time, using some kind of banking system, would be a conceivable solution to make their utilization more appealing for dermopharmaceutical testing. This study evaluated the effects of freezing at -20 °C over a period of 2 months on the structural and physicochemical properties of skin substitutes produced by tissue engineering. IntroductionLocated at the interface between the human body and the exterior environment, skin is the first line of defense against pathogens, chemicals, and harmful mechanical stimuli [1]. Due to its large surface area and its structural and functional complexity, regenerating a complete human skin represents a big challenge. Over the past years, different human skin substitute models have evolved in order to treat victims of severe burns and chronic cutaneous wounds [2]. Skin substitutes are useful in both applied and fundamental fields of research. They can be grafted as skin replacement in clinical applications or used for various types of skin tests, such as dermopharmacology and toxicology studies [2,3]. Skin substitutes properties must be as close as possible to those of normal skin [4].Over the past decade or so, the use of animals in fundamental and applied research has been subject to social debates, and this has led to the creation of more restrictive legislations, such as the addition of the Seventh Amendment to the European Union's Cosmetic Directive [4]. Utilization of skin substitutes to perform tests allowed for the elimination, or at least reduction, of controversial animal testing. Moreover, as animal skin differs in many ways to human skin, well representative skin substitutes could improve the validity of performed tests [5]. In order to increase efficiency, rapid access to skin substitutes is critical. Therefore, optimized storage methods are needed [6].Cryopreservation is an approach that has been under much investigation recently. The impact of various freezing conditions has been tested on animal and human skins. Among all tests performed, permeability analysis is one of the most commonly used in the literature. This test is based on the fact that skin, despite its crucial involvement as a barrier against exogenous material, remains permeable to most substances. AbstractBackground: Three-dimensional skin substitutes reconstructed by tissue engineering have strong potential to emulate skin conditions in vivo, although their production necessitates a relatively long period of time. Storage of cryopreserved substitutes among time, using some kind of banking system, would be a conceivable solution to make their utilization more appealing for dermo pharmaceutical testing. This study evaluated the effects of freezing at -20 °C over a period of 2 months on the structural and physicochemical properties of skin substitu...

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Cryopreservation of tissue-engineered epithelial sheets in trehalose

Cited by 50 publications

References 34 publications

Effects of Freezing on Functionality and Physicochemical Properties of A 3D-Human Skin Model

Effects of Freezing on Functionality and Physicochemical Properties of A 3D-Human Skin Model

Engineering of epidermis skin grafts using electrospun nanofibrous gelatin/polycaprolactone membranes

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