Cryopreservation of umbilical cord blood: 2. Tolerance of CD34+ cells to multimolar dimethyl sulphoxide and the effect of cooling rate on recovery after freezing and thawing

Hunt, Charles J.; Armitage, Sue; Pegg, D.E.

doi:10.1016/s0011-2240(02)00181-5

Cited by 81 publications

(54 citation statements)

References 21 publications

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“…This may be mediated by alterations in cryoprotectant concentration and cooling rate in units with nonstandard volumes. [37][38][39] Inaccuracies in MSKCC laboratory measurements might account for the observed variations in Bank-MSKCC CD34 1 cell correlation, postthaw recovery, and postthaw viability, but this is unlikely for several reasons. First, that the infused viable CD34 1 cell dose was a better predictor of neutrophil engraftment than the precryopreservation CD34 1 cell dose provides evidence that the postthaw measurement was the more accurate estimate of progenitor cell content.…”

Section: Discussionmentioning

confidence: 99%

Dominant unit CD34+ cell dose predicts engraftment after double-unit cord blood transplantation and is influenced by bank practice

Purtill¹,

Smith²,

Devlin

et al. 2014

Blood

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• Dominant unit infused viable CD34 1 cell dose determines engraftment after double-unit CBT.• Postthaw CD341 cell recovery and viability are strongly associated with differences in CB banking practices.We investigated the unit characteristics associated with engraftment after double-unit cord blood (CB) transplantation (dCBT) and whether these could be reliably identified during unit selection. Cumulative incidence of neutrophil engraftment in 129 myeloablative dCBT recipients was 95% (95% confidence interval: 90-98%). When precryopreservation characteristics were analyzed, the dominant unit CD34 1 cell dose was the only characteristic independently associated with engraftment (hazard ratio,

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Section: Discussionmentioning

confidence: 99%

Dominant unit CD34+ cell dose predicts engraftment after double-unit cord blood transplantation and is influenced by bank practice

Purtill¹,

Smith²,

Devlin

et al. 2014

Blood

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show abstract

“…For the most part, slow freezing protocols seem to be the consensus among most laboratories for cryopreservation of adult stem cells. 12,28,34,36,41 Hunt et al 124 demonstrated that even with increased concentrations of DMSO, faster cooling rates (10°C/ min and 100°C/min) resulted in significant drop-off in viability, and Donaldson et al 125 even reported a statistically significant difference in viability between cooling at 1 and 5°C/min. Similarly, previous studies with ASCs indicated significant loss in viability when the cooling rate was increased from 1 to 40°C/min.…”

Section: Freezing Thawing and Viability Assessmentmentioning

confidence: 99%

Clinical grade adult stem cell banking

2009

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“…Though single-step addition of CPAs used at concentrations of < 2M is often considered safe, adding in sequentially increasing concentrations often provides improved survival [39]. On the other hand, unloading the CPA in a single step, such as centrifugation of the cells and replacement with a CPA-free solution, is likely to lead to damaging osmotic transients as the cells undergo unrestricted cell swelling in response to the osmotic imbalance.…”

Section: Choice Of Cpamentioning

confidence: 99%

Technical Considerations in the Freezing, Low-Temperature Storage and Thawing of Stem Cells for Cellular Therapies

Hunt¹

2019

Transfus Med Hemother

105

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The commercial and clinical development of cellular therapy products will invariably require cryopreservation and frozen storage of cellular starting materials, intermediates and/or final product. Optimising cryopreservation is as important as optimisation of the cell culture process in obtaining maximum yield and a consistent end-product. Suboptimal cryopreservation can lead not only to batch-to-batch variation, lowered cellular functionality and reduced cell yield, but also to the potential selection of subpopulations with genetic or epigenetic characteristics divergent from the original cell line. Regulatory requirements also impact on cryopreservation as these will require a robust and reproducible approach to the freezing, storage and thawing of the product. This requires attention to all aspects of the application of low temperatures: from the choice of freezing container and cryoprotectant, the cooling rate employed and its mode of delivery, the correct handling of the frozen material during storage and transportation, to the eventual thawing of the product by the end-user. Each of these influences all of the others to a greater or lesser extent and none should be ignored. This paper seeks to provide practical insights and alternative solutions to the technical challenges faced during cryopreservation of cells for use in cellular therapies.

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Cryopreservation of umbilical cord blood: 2. Tolerance of CD34+ cells to multimolar dimethyl sulphoxide and the effect of cooling rate on recovery after freezing and thawing

Cited by 81 publications

References 21 publications

Dominant unit CD34+ cell dose predicts engraftment after double-unit cord blood transplantation and is influenced by bank practice

Dominant unit CD34+ cell dose predicts engraftment after double-unit cord blood transplantation and is influenced by bank practice

Clinical grade adult stem cell banking

Technical Considerations in the Freezing, Low-Temperature Storage and Thawing of Stem Cells for Cellular Therapies

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