Cryopreservation of viroid-infected chrysanthemum shoot tips

Li, Jingwei; Hosokawa, Munetaka; Nabeshima, Tomoyuki; Motoki, Ko; Yamada, Haruka; Wang, Qiao‐Chun

doi:10.1016/j.scienta.2018.09.004

Cited by 14 publications

(6 citation statements)

References 47 publications

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“…Nowadays, cryopreservation; i.e. storage of biological material at ultra-low temperature of liquid nitrogen; is considered to be the most effective long-term preservation method, not only of chrysanthemum but also other vegetatively-propagated plant species (Martinez-Montero and Harding 2015;Li et al 2019). The technology is inexpensive and safe to implement (Kulus and Zalewska 2014).…”

Section: Introductionmentioning

confidence: 99%

Cryopreservation by encapsulation-dehydration affects the vegetative growth of chrysanthemum but does not disturb its chimeric structure

Kulus

Rewers

Serocka

et al. 2019

Plant Cell Tiss Organ Cult

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Little is known about the stability of plant chimeras after exposure to liquid nitrogen. The aim of this study was to evaluate the effect of cryopreservation on the cytogenetic and genetic stability, as well as vegetative and generative growth of chrysanthemums that are a solid mutant ('Richmond') or periclinal chimeras ('Lady Orange' and 'Lady Salmon'). The following encapsulation-dehydration protocol of shoot tip cryopreservation was applied: 0.09 M sucrose concentration and 10 µM abscisic acid during a two-week preculture, followed by a 4-day osmotic dehydration and 3-hour desiccation. Both, the cryopreservation-recovered and control plants were characterized by the same relative DNA content and chromosome number (ploidy level). By applying RAPD and ISSR markers, 18 and 1 and 0 polymorphic loci within cryopreservation-derived 'Lady Orange' and 'Lady Salmon' and 'Richmond' were detected, respectively. The recovered after cryopreservation and control plants had the same colour, diameter and fresh weight of inflorescences, as well as length of ray florets. Cryopreservation also did not affect the flowering time in chrysanthemum. The biochemical assay did not reveal any alternations in the level of anthocyanins and carotenoids in flowers. It was noted, however, that some of the leaves of the cryopreservation-recovered plants were shorter and/or narrower. They had a reduced chlorophyll content, and their internodes were shorter when compared to the untreated control. The inflorescences of 'Lady Salmon' opened slower, but faded faster. In conclusion, these results illustrate the practicability of a cryopreservation method that completely protects the chimera chrysanthemum identity. Key messageCryopreservation via encapsulation-dehydration technique affects the vegetative growth of chrysanthemum during the first cultivation season but does not disturb its chimeric structure.

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Section: Introductionmentioning

confidence: 99%

Cryopreservation by encapsulation-dehydration affects the vegetative growth of chrysanthemum but does not disturb its chimeric structure

Kulus

Rewers

Serocka

et al. 2019

Plant Cell Tiss Organ Cult

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“…Chrysanthemum production has been greatly affected by CSVd and chrysanthemum chlorotic mottle viroid (CChMVd) [38,39,40]. The first step in eradicating this problem is to create viroid-free plants, and several studies have explored this approach [13,14,15,16,17,18,19]. Hosokawa et al [15,41] established a leaf primordia (LP)-free shoot apical meristem (SAM) culturing method to obtain CSVd- and CChMVd-free plants.…”

Section: Discovery Of Csvd Resistancementioning

confidence: 99%

“…Di Serio et al [66] showed that RNA-dependent RNA polymerase 6, which catalyzes the amplification of the double-stranded precursors of secondary small interfering RNAs, has a role in the surveillance mechanism, restraining the entry of PSTVd into the shoot tips of N. benthamiana . In susceptible chrysanthemums, CSVd could invade leaf primordia and cells that were very close to the apical dome [21,23] or even the outmost layer of the apical dome [13]. CSVd scarcity in shoot apices, even when CSVd was continuously provided from highly infected rootstocks, was a common characteristic in resistant progenies of “Utage” [21] and resistant cultivars including “Mari Kazaguruma” and “Sei no Issei” [23], which may suggest relatively high CSVd degradation activities in such cultivars.…”

Section: Possible Mechanisms Of Csvd Resistancementioning

confidence: 99%

“…Attempts for managing chrysanthemum stunt disease have focused on the development of early CSVd detection techniques to eliminate infected plants from the fields, on producing CSVd-free plants [13,14,15,16,17,18,19], and on the development of viroid disinfectants for pruning tools [2,20]. Recently, several studies have demonstrated the existence of resistance in commercial cultivars in Japan [21,22,23,24,25].…”

Section: Introductionmentioning

confidence: 99%

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Chrysanthemum Stunt Viroid Resistance in Chrysanthemum

Nabeshima

Matsushita

Hosokawa

2018

Viruses

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Chrysanthemum stunt viroid (CSVd) is one of the most severe threats in Chrysanthemum morifolium production. Over the last decade, several studies have reported the natural occurrence of CSVd resistance in chrysanthemum germplasms. Such CSVd-resistant germplasms are desirable for the stable production of chrysanthemum plants. Current surveys include finding new resistant chrysanthemum cultivars, breeding, and revealing resistant mechanisms. We review the progress, from discovery to current status, of CSVd-resistance studies, while introducing information on the improvement of associated inoculation and diagnostic techniques.

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“…The cryotherapy effect has been proved most evident in shoot tips infected by the phloem-limited virus (Bi et al 2018;Zhao et al 2019), and was then followed by viruses that cannot infect the apical meristem (Li et al 2016). As for viruses or viroid that infect the apical meristem, no or very low cryotherapy effect could be obtained, making shoot tip cryopreservation a reliable means for long-term preservation of these pathogens (Wang et al 2018c; Li et al 2019).…”

Section: Introductionmentioning

confidence: 99%

Vitrification Cryo-Foil for Apple Cryopreservation and the Seesaw Effect Between Shoot Recovery and Virus Eradication

Wang

Jiang

et al. 2021

Preprint

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Improvements of existing cryopreservation protocols are necessary to facilitate long-term preservation of plant germplasm and the cryotherapy-effect of pathogen eradication. This study reported a vitrification (V) cryo-foil/plate methods for cryopreservation of shoot tips and cryotherapy effect in ‘Pink Lady’ apple. In V cryo-foil/plate protocols, shoot tips were first attached onto aluminum foils/plates using calcium alginate before other procedures. Shoot tips cryopreserved by V cryo-foil required 6.1 weeks to fully recover and 53% of shoot regrowth was obtained, comparable to the Dv cryopreservation. Similar regrowth levels were produced between applying V cryo-foil and Dv cryopreservation to another 4 Malus genotypes. Histological observations in shoot tips cryopreserved by Dv and V cryo-foil found only those with surviving apical dome and leaf primordia (LPs) could recover after cryopreservation. In apical meristem of shoot tips cryopreserved by Dv and V cryo-foil, higher surviving probability was detected from the V cryo-foil protocol, and the young LPs showed the highest level of surviving. Virus detection in cryo-derived plants showed apple stem grooving virus and apple chlorotic leaf spot virus were all preserved after cryopreservation, and higher eradication efficiency of apple stem pitting virus (70%) was produced by Dv than the 55% of V cryo-foil. These results supported applying V cryo-foil as an improvement to the widely applied Dv method in shoot tip cryopreservation, and also revealed a seesaw mode between shoot recovery and cryotherapy effect. Once the seesaw moves to increase the recovery after cryopreservation, the cryotherapy-effect on the other side would be decreased.

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Cryopreservation of viroid-infected chrysanthemum shoot tips

Cited by 14 publications

References 47 publications

Cryopreservation by encapsulation-dehydration affects the vegetative growth of chrysanthemum but does not disturb its chimeric structure

Cryopreservation by encapsulation-dehydration affects the vegetative growth of chrysanthemum but does not disturb its chimeric structure

Chrysanthemum Stunt Viroid Resistance in Chrysanthemum

Vitrification Cryo-Foil for Apple Cryopreservation and the Seesaw Effect Between Shoot Recovery and Virus Eradication

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