Cryopreservation with dimethyl sulfoxide sustains partially the biological function of osteochondral tissue

Egli, Rainer J.; Sckell, Axel; Fraitzl, Christian R.; Félix, R.; Ganz, Reinhold; Hofstetter, Willy; Leunig, Michael

doi:10.1016/s8756-3282(03)00192-3

Cited by 28 publications

(23 citation statements)

References 31 publications

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“…35,36 Antigen retrieval was performed with 2% hyaluronidase (Sigma-Aldrich) in PBS for 30 min followed by blockage of endogenous peroxidase for 10 min with 0.3% H 2 O 2 . The sections were incubated with rabbit anti-bovine collagen type II polyclonal antibody (diluted 1:40, AB746P, Chemicon International) for 60 min.…”

Section: Immunohistochemistrymentioning

confidence: 99%

Hypoxic expansion promotes the chondrogenic potential of articular chondrocytes

Egli

Bastian

Ganz

et al. 2008

Journal Orthopaedic Research

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For cell-based cartilage repair strategies, an ex vivo expansion phase is required to obtain sufficient numbers of cells needed for therapy. Although recent reports demonstrated the central role of oxygen for the function and differentiation of chondrocytes, a beneficial effect of low oxygen concentrations during the expansion of the cells to further improve their chondrogenic capacity has not been investigated. Therefore, freshly harvested bovine articular chondrocytes were grown in two-dimensional monolayer cultures at 1.5% and 21% O 2 and redifferentiation was subsequently induced in three-dimensional micromass cultures at 1.5%, 5%, and 21% O 2 . Cells expanded at 1.5% O 2 were characterized by low citrate synthase (aerobic energy metabolism)-and high LDH (anaerobic energy metabolism)-activities, suggesting an anaerobic energy metabolism. Collagen type II mRNA was twofold higher in cells expanded at 1.5% as compared to expansion at 21% O 2 . Micromass cultures grown at 21% O 2 showed up to a twofold increase in the tissue content of glycosaminoglycans when formed with cells expanded at 1.5% instead of 21% O 2 . However, no differences in the levels of transcripts and in the staining for collagen type II protein were observed in these micromass cultures. Hypoxia (1.5% and 5% O 2 ) applied during micromass cultures gave rise to tissues with low contents of glycosaminoglycans only. In vivo, the chondrocytes are adapted to a hypoxic environment. Taking this into account, by applying 1.5% O 2 in the expansion phase in the course of cell-based cartilage repair strategies, may result in a repair tissue with higher quality by increasing the content of glycosaminoglycans. ß

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Section: Immunohistochemistrymentioning

confidence: 99%

Hypoxic expansion promotes the chondrogenic potential of articular chondrocytes

Egli

Bastian

Ganz

et al. 2008

Journal Orthopaedic Research

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“…This allows for storage of the grafts for up to 42 days, or so-called "prolonged fresh" grafts. 15,63 These grafts have been shown to have minimal immune response, preserved chondrocyte viability, and enhanced revascularization of the bone. 47,61 Furthermore, this improves the safety of these grafts by allowing more time for bacteriologic and DNA polymerase chain reaction (PCR) screening.…”

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confidence: 99%

Prospective Evaluation of Prolonged Fresh Osteochondral Allograft Transplantation of the Femoral Condyle

et al. 2007

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“…1), and better suited to the biological properties of fresh musculoskeletal allograft as proved in other reported research (Egli et al 2003;Ball et al 2004;Rohde et al 2004;Pennock et al 2006a, b;Armstrong and Mow 1982). Once the osteochondral allografts were frozen no significant deep freezing time dependent difference was noticed in the tested mechanical properties of the osteochondral allografts.…”

Section: Discussionmentioning

confidence: 69%

The effects of prolonged deep freezing on the biomechanical properties of osteochondral allografts

et al. 2008

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Musculo-skeletal allografts sterilized and deep frozen are among the most common human tissue to be preserved and utilized in modern medicine. The effects of a long deep freezing period on cortical bone has already been evaluated and found to be insignificant. However, there are no reports about the influences of a protracted deep freezing period on osteochondral allografts. One hundred osteochondral cylinders were taken from a fresh specimen and humeral heads of 1 year, 2 years, 3 years and 4 year old bones. Twenty chips from each period, with a minimum of 3 chips per humeral head. Each was mechanically tested by 3 point compression. The fresh osteochondral allografts were significantly mechanically better than the deep frozen osteochondral allografts. There was no statistical significant time dependent difference between the deep frozen groups in relation to the freezing period. Therefore, we conclude that, from the mechanical point of view deep freezing of osteochondral allografts over a period of 4 years, is safe without further deterioration of the biomechanical properties of the osteochondral allografts.

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Cryopreservation with dimethyl sulfoxide sustains partially the biological function of osteochondral tissue

Cited by 28 publications

References 31 publications

Hypoxic expansion promotes the chondrogenic potential of articular chondrocytes

Hypoxic expansion promotes the chondrogenic potential of articular chondrocytes

Prospective Evaluation of Prolonged Fresh Osteochondral Allograft Transplantation of the Femoral Condyle

The effects of prolonged deep freezing on the biomechanical properties of osteochondral allografts

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