The effects of prolonged deep freezing on the biomechanical properties of osteochondral allografts

Rozen, Benjamin; Brosh, Tamar; Salai, Moshe; Herman, Amir; Dudkiewicz, Israel

doi:10.1007/s10561-008-9106-z

Cited by 18 publications

(12 citation statements)

References 32 publications

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“…No effect of storage period on mechanical properties after 1 year of storage compared to fresh bones was reported [36] . On the other hand, deterioration in mechanical properties [37] and improvement in strength [38] after freezing were also found. In any case, bones mechanical properties were not tested in vivo and the results are based on ex vivo measurements or evaluation based on imaging data.…”

Section: Discussionmentioning

confidence: 98%

Tensile Mechanical Properties of Swine Cortical Mandibular Bone

et al. 2014

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Temporary orthodontic mini implants serve as anchorage devices in orthodontic treatments. Often, they are inserted in the jaw bones, between the roots of the teeth. The stability of the mini implants within the bone is one of the major factors affecting their success and, consequently, that of the orthodontic treatment. Bone mechanical properties are important for implant stability. The aim of this study was to determine the tensile properties of the alveolar and basal mandible bones in a swine model. The diametral compression test was employed to study the properties in two orthogonal directions: mesio-distal and occluso-gingival. Small cylindrical cortical bone specimens (2.6 mm diameter, 1.5 mm thickness) were obtained from 7 mandibles using a trephine drill. The sites included different locations (anterior and posterior) and aspects (buccal and lingual) for a total of 16 specimens from each mandible. The load-displacement curves were continuously monitored while loading half of the specimens in the oclluso-gingival direction and half in the mesio-distal direction. The stiffness was calculated from the linear portion of the curve. The mesio-distal direction was 31% stiffer than the occluso-gingival direction. The basal bone was 40% stiffer than the alveolar bone. The posterior zone was 46% stiffer than the anterior zone. The lingual aspect was stiffer than the buccal aspect. Although bone specimens do not behave as brittle materials, the diametral compression test can be adequately used for determining tensile behavior when only small bone specimens can be obtained. In conclusion, to obtain maximal orthodontic mini implant stability, the force components on the implants should be oriented mostly in the mesio-distal direction.

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Section: Discussionmentioning

confidence: 98%

Tensile Mechanical Properties of Swine Cortical Mandibular Bone

et al. 2014

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“…No change of the biomechanical properties of bone was found after up to 5 years of storage at -80 °C [19]. The duration of freezing did not affect the biomechanical properties of human osteochondral grafts as well [46]. Our previous study, which in contrast to the study of Salai et al [19], studied two different temperatures, compared the effect of storage for up to 2 years at -80 °C to a higher temperature at -20 °C; no adverse effect of deep freezing onto biomechanical properties even after…”

Section: Disclosure Statementmentioning

confidence: 99%

Effect of Storage Temperature on Allograft Bone

Fölsch¹,

Mittelmeier²,

Bilderbeek³

et al. 2012

Transfus Med Hemother

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Background: The recommendations for storage temperature of allogeneic bone are varying between –20 °C and –70 °C and down to –80 °C. The necessary temperature of storage is not exactly defined by scientific data, and the effect of different storage temperatures onto the biomechanical and the biological behavior is discussed controversially. Methods: The historical development of storage temperature of bone banks is described. A survey on literature concerning the biomechanical and biological properties of allograft bone depending on the procurement and storage temperature is given as well as on national and international regulations on storage conditions of bone banks (European Council, American Association of Tissue Banks (AATB), European Association of Tissue Banks (EATB)). Results: Short-term storage up to 6 months is recommended with –20 °C and –40 °C for a longer period (AATB), and EATB recommends storage at –40 °C and even –80 °C while the regulations of the German German Medical Association (Bundesärztekammer) from 2001 recommend storage at –70 °C. Duration of storage at –20 °C can be maintained at least for 2 years. The potential risk of proteolysis with higher storage temperatures remains, but a definite impairment of bone ingrowth due to a storage at –20 °C was not shown in clinical use, and no adverse biomechanical effects of storage at –20 °C could be proven. Conclusion: Biomechanical studies showed no clinically relevant impairment of biomechanical properties of cancellous bone due to different storage temperatures. Sterilization procedures bear the advantage of inactivating enzymatic activity though reducing the risk of proteolysis. In those cases a storage temperature of –20 °C can be recommended for at least a period of 2 years, and the risk of undesired effects seems to be low for native unprocessed bone.

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“…To prepare for testing, each limb was thawed separately at room temperature (approximately 24 hours). A single freeze‐thaw cycle does not alter structural or biochemical properties of articular cartilage from osteochondral specimens 14,15 . After thawing, the muscle and connective tissue surrounding the knee joint was removed and the joint was disarticulated to expose the distal femur.…”

Section: Methodsmentioning

confidence: 99%

“…A single freeze-thaw cycle does not alter structural or biochemical properties of articular cartilage from osteochondral specimens. 14,15 After thawing, the muscle and connective tissue surrounding the knee joint was removed and the joint was disarticulated to expose the distal femur. The femoral condyles and trochlea, medial and lateral menisci, proximal tibia cartilage, collateral, and cruciate ligaments, and synovial membrane were grossly examined to ensure there were no articular cartilage or bone defects, or other evidence of joint disease prior to testing.…”

Section: Sample Preparationmentioning

confidence: 99%

Mechanical, biochemical, and morphological topography of ovine knee cartilage

Risch

Easley

McCready

et al. 2020

Journal Orthopaedic Research

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The knee is the most common site for translational cartilage research in sheep, though topographic features of articular cartilage across surfaces are unspecified. We aimed to characterize the mechanical, morphological, and biochemical properties of articular cartilage across ovine knee surfaces and document variations between and within surface locations. Regions of interest (ROIs) were delineated across surfaces of 10 healthy ovine knees. Articular cartilage at each ROI was measured for creep indentation, thickness, and glycosaminoglycan (GAG) and collagen content. Variables were compared between surface locations (trochlea, and lateral [LFC] and medial [MFC] femoral condyles) and between ROIs within each surface location. Correlations between variables were also assessed. Articular surface location had a significant effect on creep (P < .

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The effects of prolonged deep freezing on the biomechanical properties of osteochondral allografts

Cited by 18 publications

References 32 publications

Tensile Mechanical Properties of Swine Cortical Mandibular Bone

Tensile Mechanical Properties of Swine Cortical Mandibular Bone

Effect of Storage Temperature on Allograft Bone

Mechanical, biochemical, and morphological topography of ovine knee cartilage

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