Cryopreserved human haematopoietic stem cells retain engraftment potential after extended (5–14 years) cryostorage

Spurr, Elisabeth E; Wiggins, N; Marsden, Kate; Lowenthal, RM; Ragg, SJ

doi:10.1016/s0011-2240(02)00027-5

Cited by 64 publications

(37 citation statements)

References 25 publications

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“…23 Moreover, the NC and CD34 þ cell recovery and the viability were not linked with the duration of freezing in this study, consistent with data showing that HSC retain engraftment potential after extended (5-14 years) cryostorage. 21,24,25 Interestingly, we found a significant decrease in NC recovery and viability in patients with myeloma. It could be related to the higher granulocyte count in the apheresis collection in myeloma than in other patients (48 versus 40%, P ¼ 0.0001).…”

Section: Discussionmentioning

confidence: 58%

See 1 more Smart Citation

Recovery, viability and clinical toxicity of thawed and washed haematopoietic progenitor cells: analysis of 952 autologous peripheral blood stem cell transplantations

Foïs

Desmartin

Benhamida

et al. 2007

Bone Marrow Transplant

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Cryopreservation and thawing of haematopoietic stem cells are associated with cell loss and infusion-related toxicities. We analysed viability, total nucleated cell (TNC) and CD34 þ cell recovery, and infusion-related toxicities of 952 thawed and washed products. Mean TNC and CD34 þ viable cells recoveries were 55.9718.6 and 98.0736.5%, respectively. Mean cell viability was 68.25718.9%. TNC recovery was correlated with viability but independent of the initial nucleated cell concentration. No difference in TNC recovery or viability was observed according to underlying diseases, except for myeloma, for which these variables were significantly lower (Po0.05). CD34 þ cell recovery was not correlated with viability or CD34 þ initial count and was similar for all diseases. Cryostorage duration was not associated with cell loss. Immediate adverse events occurred in 169 patients (19%) and were moderate (grade I or II) for the majority of patients. Clinical toxicity was associated with a higher infused cell number and the presence of clumps in infused bags. The washing procedure of cell products lead to a low rate of adverse events, but patients transplanted with high cell numbers or bags in which clumps were identified are predisposed to such complications.

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Section: Discussionmentioning

confidence: 58%

“…Although it has been shown that haematopoietic stem cells may retain engraftment capacity after extended cryostorage, 21 the effect of storage duration on cell recovery and viability was analysed in two groups as follows: one transplanted with cells cryopreserved for less than 4 months and another for more than 4 months.…”

Section: Patientsmentioning

confidence: 99%

Recovery, viability and clinical toxicity of thawed and washed haematopoietic progenitor cells: analysis of 952 autologous peripheral blood stem cell transplantations

Foïs

Desmartin

Benhamida

et al. 2007

Bone Marrow Transplant

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“…15,16 For each study, it is possible that very high initial stem cell yields, even with significant loss over time, could still give engraftment after a relatively short cryo-storage time of 2.8 years. Spurr et al 13 examined surrogate markers of potential engraftment such as CD34 þ cells and CFU-GM after a median of 9.5 years and found that deterioration of stem cell quality and viability may occur and that many of the long-term stored SCPs were not adequate for transplantation. They did not directly compare the original and current results for each individual specimen, but the data indicated that the stem cells stored the longest had the lowest CD34 þ cell counts and CFU-GM.…”

Section: Discussionmentioning

confidence: 99%

“…[5][6][7][8] However, studies on PB-or BM-derived stem cells have been limited and predominantly analysed the effects of relatively short (median p2 years) storage. [9][10][11][12] A single study has suggested that SCPs stored for longer periods (median 9 years) may be inadequate for transplant 13 but did not compare pre-and post-storage results for individual samples.…”

Section: Introductionmentioning

confidence: 99%

Relative recovery of haematopoietic stem cell products after cryogenic storage of up to 19 years

Fernyhough

Buchan

McArthur

et al. 2012

Bone Marrow Transplant

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There is an increasing trend towards long-term frozen storage of haematopoietic stem cells. For such stem cells, harvested from peripheral blood (PB) or BM, it is not known if stem cell viability decreases with time. In this study, 31 separate bags of stem cell product (SCP) stored for 11-19 years (median 15 years) were assessed for total nucleated cell (TNC) count, colony forming unitgranulocyte/macrophage (CFU-GM), CD34 þ cell count and cell viability. The results were compared with the initial results obtained for the products at the time of stem cell harvest, and the percentage recovery of each parameter was plotted against time. Recovery of TNC, CD34 þ cell count and cell viability decreased with time (P ¼ o0.01) but CFU-GM did not. This study shows that SCPs harvested from PB and BM do deteriorate with long-term storage. This could have an impact on rates of engraftment.

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“…La dé cision validé e en RCP : contamination microbiologique : discussion en RCP avec avis du comité de lutte contre les infections nosocomiales : duré e de stockage supé rieure à 15 ans en prenant en compte le rapport bé né fice/risque pour le malade [1,2].…”

Section: Destruction Des Produits Cellulaires Alloge´niques [Csh Fraunclassified

Modalités de conservation et de destruction des produits cellulaires cryopréservés : recommandations de la SFGM-TC

Calmels

Boulanger²,

Baudoux³

et al. 2014

Pathologie Biologie

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Cryopreserved human haematopoietic stem cells retain engraftment potential after extended (5–14 years) cryostorage

Cited by 64 publications

References 25 publications

Recovery, viability and clinical toxicity of thawed and washed haematopoietic progenitor cells: analysis of 952 autologous peripheral blood stem cell transplantations

Recovery, viability and clinical toxicity of thawed and washed haematopoietic progenitor cells: analysis of 952 autologous peripheral blood stem cell transplantations

Relative recovery of haematopoietic stem cell products after cryogenic storage of up to 19 years

Modalités de conservation et de destruction des produits cellulaires cryopréservés : recommandations de la SFGM-TC

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