Cryoprotective effects of various saccharides on cryopreserved mouse sperm from various strains

Hino, Toshiaki; Takabe, Miho; Suzuki-Migishima, Rika; Yokoyama, Minesuke

doi:10.1111/j.1447-0578.2007.00190.x

Cited by 13 publications

(5 citation statements)

References 30 publications

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“…Carbohydrates act not only as an energy source in cryodiluents but also play a role in maintaining osmolarity and provide cryoprotection by obstructing intracellular ice formation through cellular dehydration [ 30 ]. It has been demonstrated in other species that disaccharides provide better cryoprotection to spermatozoa than monosaccharides (boar, [ 31 ]; mouse, [ 32 ]). While the reason behind this has not been elucidated, one proposed mechanism is the higher osmotic pressure exerted by the disaccharides, more effectively reducing the formation of intracellular ice crystals [ 33 ].…”

Section: Discussionmentioning

confidence: 99%

Adjusting cryodiluent composition for improved post-thaw quality of rabbit spermatozoa

et al. 2017

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Improved fertility following artificial insemination with frozen-thawed spermatozoa would offer rabbit producers faster genetic improvement. Previous work investigating cryoprotectants for rabbit spermatozoa have reported inconsistent results. Semen was collected from three rabbit bucks by artificial vagina and frozen using a standard procedure with varied cryodiluent components. Post-thaw analysis encompassed motility, sperm kinematic parameters and acrosome and membrane integrity. Spermatozoa were evaluated at 0, 2 and 4 h after thawing. Experiment 1 compared diluents with 3.5% dimethyl sulfoxide (DMSO), 1.5% acetamide, 1.75% DMSO + 0.75% acetamide or 3.5% DMSO + 1.5% acetamide. The treatment that resulted in the highest post-thaw motility (P<0.001) and acrosome integrity (P<0.001) was DMSO alone. Experiment 2 compared 3.5, 7 and 10% DMSO in the cryodiluent. The best post-thaw sperm motility (P<0.001) and linearity (P = .002) was in 3.5% DMSO, while 10% DMSO afforded higher acrosome/membrane integrity at this last time point (P<0.05). Experiment 3 varied the cryodiluent to contain either 9 or 17% egg yolk or 9 or 17% low density lipoproteins extracted from whole egg yolk. The treatment with the best post-thaw result was 17% egg yolk (motility, P = 0.01; acrosome/membrane integrity, P<0.001). Experiment 4 compared different carbohydrates in the cryodiluent; 50 mM glucose (TCG), 25 mM glucose with 25 mM sucrose (TCGS low), or 50 mM glucose with 50 mM sucrose (TCGS high). When data were pooled across time points, TCG had significantly higher motility than TCGS high (P = 0.021), but was not different from TCGS low. However, TCG had fewer spermatozoa with intact acrosomes and membranes than both TCGS low and TCGS high (P = .002). Put together, these results indicate that the best cryodiluent for rabbit spermatozoa frozen under the conditions used in this paper is with 7% DMSO and 17% egg yolk in a base medium containing 25 mM glucose and 25 mM sucrose.

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Section: Discussionmentioning

confidence: 99%

Adjusting cryodiluent composition for improved post-thaw quality of rabbit spermatozoa

et al. 2017

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“…TYH is an older IVF medium developed for mice and is still widely used due to its ability to support fertilization [9]. On the other hand, HTF is the first specific medium for human IVF, and mouse oocytes of various strains have been successfully fertilized using it [31,32].…”

Section: Mediamentioning

confidence: 99%

“…Successful cryopreservation of mouse sperm was reported in 1990 using raffinose with glycerol [1], ME2SO [2] or skim milk [3]. Since then, cryoprotectant solutions and freezing methods for mouse sperm have been investigated [4][5][6][7][8][9][10][11]. At present, the 18% raffinose plus 3% skim milk method [12] is relied upon in most laboratories.…”

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confidence: 99%

Marked Improvement of Fertility of Cryopreserved C57BL/6J Mouse Sperm by Depletion of Ca2+ in Medium

Suzuki-Migishima

Hino

Takabe

et al. 2009

Journal of Reproduction and Development

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Abstract. Cryopreservation of mouse sperm is useful for maintaining various strains. However, fertility generally decreases after freezing. In particular, the fertility of cryopreserved C57BL/6J sperm is very low. To improve the fertility of frozen sperm, we examined the efficiencies of various media used for sperm preincubation (SP) and in vitro fertilization (IVF) in frozen C57BL/6J sperm. In this study, SP medium was examined for efficiency of fertility with respect to content, especially calcium (Ca 2+ ), phosphate (PO4 3-) and lactate. In all media containing no Ca 2+ , including medium lacking Ca 2+ , lacking Ca 2+ and PO4 3-, lacking Ca 2+ and lactate and lacking Ca 2+ , PO4 3-and lactate, high IVF rates were obtained (79, 69, 76 and 71%, respectively). On the other hand, the rates for media containing Ca 2+ were significantly lower (30-38%, P<0.05). After transfer, 41-50% of newborns were obtained in all media containing no Ca 2+ .In conclusion, preincubation of thawed sperm in medium containing no Ca 2+ markedly improved the fertility of cryopreserved C57BL/6J sperm. These results indicate that the present method of IVF using medium with no Ca 2+ is practical for use in cryopreserved C57BL/6J sperm. Key words: C57BL/6, Calcium, Cryopreservation, In vitro fertilization, Sperm (J. Reprod. Dev. 55: [386][387][388][389][390][391][392] 2009) ryopreservation of mouse sperm is useful because it is simple, rapid and inexpensive. A large number of sperm can be frozen immediately after collection from a male. Females are required only when the frozen sperm are thawed for fertilization. Successful cryopreservation of mouse sperm was reported in 1990 using raffinose with glycerol [1], ME2SO [2] or skim milk [3]. Since then, cryoprotectant solutions and freezing methods for mouse sperm have been investigated [4][5][6][7][8][9][10][11]. At present, the 18% raffinose plus 3% skim milk method [12] is relied upon in most laboratories. However, with this technique, the fertility of cryopreserved sperm is far less than that of fresh sperm, especially with inbred mice [13][14][15][16]. In particular, fertility decreases markedly in C57BL/6J, a principal inbred strain used for transgenesis studies. Frozen C57BL/6J sperm exhibit acrosome damage with loss of contents caused by the freeze-thaw procedure [17], and almost no sperm can penetrate the zona pellucida. When partial zona pellucida nicking [18,19] or intracytoplasmic sperm injection (ICSI) [20,21] is performed to increase fertility, post-thawed C57BL/6J sperm can fertilize a high percentage of eggs. Improved IVF rates with intact oocytes have rarely been reported using motile sperm separated from thawed suspension [16,22] or methyl-beta-cyclodextrin (MBCD) stimulating cholesterol efflux from thawed sperm [23]. Successful IVF with fresh sperm depends on the media used. Various IVF media have been developed, and the effects of media on capacitation and IVF have been investigated. However, the effects of media on frozen mouse sperm have not been investigated.In this s...

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“…Of the many disaccharides, sucrose has been widely used in the cryopreservation of sturgeon sperm (Alavi, Cosson, Karami, Amiri, & Akhoundzadeh, ; Billard et al., ; Boryshpolets et al., ; Tsvetkova, Cosson, Linhart, & Billard, ). Trehalose has reported to improve post‐thaw sperm characteristics in mammals such as sheep (Aisen et al., ), mice (Hino, Takabe, Suzuki‐Migishima, & Yokoyama, ), dog (Yildiz, Kaya, Aksoy, & Tekeli, ), boar (Gutierrez‐Perez, Juarez‐Mosqueda Mde, Carvajal, & Ortega, ), cattle (Oh et al., ) and so on. Trehalose is also used in the cryopreservation of some fish sperm (Lichtenstein, Elisio, & Miranda, ; Miyaki, Nakano, Ohta, & Kurokura, ), but has not yet been studied in sturgeon.…”

Section: Introductionmentioning

confidence: 99%

Disaccharide combinations and the expression of enolase3 and plasma membrane Ca²⁺ATPase isoform in sturgeon sperm cryopreservation

et al. 2018

Reprod Domestic Animals

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Acipenser sinensis and Acipenser dabryanus are critically endangered species, so germplasm conservation via cryopreservation of sperm is necessary. Disaccharides can act as membrane-impermeable cryoprotectants, and enolase3 (ENO3) and plasma membrane Ca ATPase isoform (PMCA2) are proteins associated with sperm quality. We considered seven characteristics of sperm quality in cultured brood stock from A. sinensis and A. dabryanus. We tested use of sucrose or trehalose alone and in combination at different concentrations for cryopreservation of A. dabryanus sperm. A low concentration of sucrose plus trehalose (S T ) was optimal. Mixing of the extender with sucrose, lactose, or trehalose alone or with pairwise mixtures revealed that a mixture of lactose and trehalose (L T ) gave the best results for both A. sinensis and A. dabryanus. Enolase3 and PMCA2 expression levels were measured in cryopreserved A. sinensis sperm via Western blotting. Relative ENO3 and PMCA2 expression levels were examined, and the relationship between disaccharide composition, sperm quality and protein expression was explored in A. sinensis. The results showed that relative ENO3 and PMCA2 expression levels were the highest at L T in cryopreserved A. sinensis sperm. There were significant positive correlations between ENO3 expression and percentage membrane integrity, and between PMCA2 expression and sperm motility parameters (percentage of motile sperm, curvilinear velocity, straight-line velocity and average path velocity; p < .05) in cryopreserved A. sinensis sperm. Our results indicate the optimal disaccharide combination and concentrations for cryopreservation of A. sinensis and A. dabryanus sperm and suggest that ENO3 and PMCA2 expression levels could serve as a valuable indicator of sperm quality in A. sinensis.

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Cryoprotective effects of various saccharides on cryopreserved mouse sperm from various strains

Cited by 13 publications

References 30 publications

Adjusting cryodiluent composition for improved post-thaw quality of rabbit spermatozoa

Adjusting cryodiluent composition for improved post-thaw quality of rabbit spermatozoa

Marked Improvement of Fertility of Cryopreserved C57BL/6J Mouse Sperm by Depletion of Ca2+ in Medium

Disaccharide combinations and the expression of enolase3 and plasma membrane Ca²⁺ATPase isoform in sturgeon sperm cryopreservation

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Cryoprotective effects of various saccharides on cryopreserved mouse sperm from various strains

Cited by 13 publications

References 30 publications

Adjusting cryodiluent composition for improved post-thaw quality of rabbit spermatozoa

Adjusting cryodiluent composition for improved post-thaw quality of rabbit spermatozoa

Marked Improvement of Fertility of Cryopreserved C57BL/6J Mouse Sperm by Depletion of Ca2+ in Medium

Disaccharide combinations and the expression of enolase3 and plasma membrane Ca2+ATPase isoform in sturgeon sperm cryopreservation

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Disaccharide combinations and the expression of enolase3 and plasma membrane Ca²⁺ATPase isoform in sturgeon sperm cryopreservation