Cryptic functions of enzymes in chemical catalysis

Previero, A.; Ma, Che; Galzigna, L

doi:10.1007/bf00799030

Cited by 5 publications

(2 citation statements)

References 14 publications

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“…The sequences of these peptides share a consensus octapeptide containing a Ser residue directly involved in the hydrolytic reaction (Dayhoff et al 1972). The catalytic sites of several proteases have also been isolated and characterized by these means and likewise contain a con- Dayhoff et al 1972Krisch 1971Augusteyn et al 1969Heymann 1980, Previero et al 1983 sensus octapeptide including an invariant Ser (Young et al 1978). Moreover, there is a three-in-eight match between the two consensus sequences, suggesting their origin from a common ancestor (Neurath 1984).…”

Section: Introductionmentioning

confidence: 99%

On the origins of esterases.

Myers

Richmond²,

Oakeshott³

1988

Molecular Biology and Evolution

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Comparisons among the primary sequences of five cloned eukaryotic esterases reveal two distinct lineages, neither bearing any significant overall sequence similarity to the functionally related serine protease multigene family. We have not eliminated the possibility that the esterases may have residual conformational similarities to the serine proteases. However, our profile analysis and analyses of the predicted conformations of the esterases reveal little similarity to the serine proteases. Four of the esterase proteins share 27%-53% overall sequence similarity and evidence of a catalytic mechanism involving the same Arg-Asp-Ser or His-Asp-Ser charge relay. We propose that these four esterases, three of them cholinesterases, form part of a multigene family essentially separate from the serine proteases.

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Section: Introductionmentioning

confidence: 99%

On the origins of esterases.

Myers

Richmond²,

Oakeshott³

1988

Molecular Biology and Evolution

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“…As already reported (8), separation of esterase and amidase activities can be accomplished either by inducing specific conformational changes in the enzyme molecule (9) or by using appropriate substrates (lo), e.g. esters of 1 -methyl-3,4-dihydro-P-carboline-3-carboxylic acid (MDC).…”

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confidence: 99%