Chymotrypsin catalysed synthesis of fluorescent amino acid amide and peptide derivatives

Abstract

Chymotrypsin catalyses a condensation reaction between l-methyl-3,4-dihydrofl-carboline-3-methyl carboxylate and amino acid amides or peptides, yielding firlorescent derivatives. During the peptide bond formation, the enzyme ensures the reaction's steric control of both carboxyl and amino components.