Synthesis of peptides catalysed by enzymes: A practical overview

Non-covalent enzyme-polyelectrolyte complexes as self-buffered catalysts for use in low-water organic media

“…Polar solvents are often favoured for protease reactions, on grounds of reactant solubility. Many workers have used alpha-chymotrypsin in acetonitrile with low water contents (Schellenberger & Jakubke, 1991;Sinisterra and Alcantara, 1993;Gill et al, 1996), which we have chosen for this study.…”