Cryptic species and population genetic structure of <i>Plasmopara viticola</i> in São Paulo State, Brazil

Camargo, Meyriele Pires de; Hong, Cheng‐Fang; Amorim, Lílian; Scherm, H.

doi:10.1111/ppa.12993

Cited by 14 publications

(13 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For instance, lower clonal fraction (0.04) and higher genotypic diversity (G = 40.69) were observed in Rio Grande do Sul compared to São Paulo (clonal fraction = 0.81; G = 2.98). A recent study also revealed that P. viticola populations from São Paulo had low genetic diversity, where 19 of the 55 MLGs were clonal and represented 93% of the isolates sampled (Camargo et al, 2019). Meanwhile, the high diversity observed in Rio Grande do Sul is similar to that in Europe, where sexual reproduction appears to play a major role in downy mildew epidemics (Gobbin et al, 2006;Delmas et al, 2017).…”

Section: Discussionmentioning

confidence: 96%

“…Conversely, the climate in São Paulo is favorable all year-round for grapevine growth and green tissues are present during almost the entire year due to the double pruning system commonly used in the state. Thus, P. viticola reproduces and survives predominantly asexually on green tissues (Camargo et al, 2019). Due to the mild winter in South Africa, P. viticola populations often experience bottlenecks at the end of the growing season and a few highly adapted genotypes can survive between seasons as asexual spores or as vegetative mycelium (Koopman et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

“…Meanwhile, the climatic conditions in South-eastern Brazil are favorable for grapevine growth all-year-round, allowing two harvests a year through double pruning management (Pommer, 2006). The double cropping system increases yield significantly, but may serve as a green bridge for pathogens, where P. viticola appears to survive predominantly asexually between seasons (Camargo et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The Climate-Driven Genetic Diversity Has a Higher Impact on the Population Structure of Plasmopara viticola Than the Production System or QoI Fungicide Sensitivity in Subtropical Brazil

et al. 2020

View full text Add to dashboard Cite

Downy mildew, caused by Plasmopara viticola, is the main disease affecting vineyards in subtropical Brazil. Here, we collected 94 P. viticola isolates from four organic and conventional vineyards in the two main grape-growing states of Brazil to evaluate the sensitivity to the quinone outside inhibitor (QoI) azoxystrobin by pheno-and genotyping assays. The impact of location, production system and sensitivity to QoI fungicides on the population genetics and structure of P. viticola was determined using 10 microsatellite markers. Cytochrome b sequencing revealed that 28 and 100% of the isolates from vineyards under organic and conventional management carried the G143A mutation, respectively. The G143A mutation was associated with high levels of azoxystrobin resistance. Three out of the 94 isolates analyzed carried the M125I alteration, not previously described in P. viticola, which was associated with a five-fold reduction in azoxystrobin sensitivity compared to wild-type isolates. Haplotype network analysis based on cytochrome b gene sequences suggested that the Brazilian populations are more closely related to the European than the North American population. A total of six haplotypes were identified, with two of them carrying the G143A mutation. Microsatellite analysis revealed high allelic and genotypic variation among the four populations. Population differentiation analyses indicated that state of origin directly influences the population biology of P. viticola, while production system and QoI sensitivity have little effect. Great genetic diversity, sexual reproduction and high levels of admixture were observed in Rio Grande do Sul State. In contrast, populations in São Paulo State were dominated by a few clonal genotypes, and no admixed genotype was detected between the two genetic pools identified in the state. This study raises the hypothesis that winter weather conditions influence the overwinter survival strategy with profound effects in the population biology of P. viticola.

show abstract

Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The Climate-Driven Genetic Diversity Has a Higher Impact on the Population Structure of Plasmopara viticola Than the Production System or QoI Fungicide Sensitivity in Subtropical Brazil

et al. 2020

View full text Add to dashboard Cite

show abstract

“…viticola is <20 m per secondary disease cycle (Gobbin et al ., 2005). However, population genetic structure studies have demonstrated that identical multilocus genotypes can be observed at distant locations, indicating that long‐distance dispersal may be possible (Camargo et al ., 2019).…”

Section: Discussionmentioning

confidence: 99%

Multiple resistance of Plasmopara viticola to QoI and CAA fungicides in Brazil

Santos

Fraaije

Garrido³

et al. 2020

Plant Pathology

View full text Add to dashboard Cite

Grapevine downy mildew, caused by Plasmopara viticola, is commonly controlled by quinone outside inhibitor (QoI) and carboxylic acid amide (CAA) fungicides. QoI resistance is conferred by a mutation resulting in a G143A alteration in cytochrome b, while CAA resistance is associated with a G1105S mutation in cellulose synthase 3. The aims of this study were to (a) verify the presence of QoI and CAA resistance in P. viticola in Brazil, (b) develop a single nucleotide polymorphism (SNP) detection pyrosequencing assay for quantitative detection of G143A, and (c) determine the geographic prevalence and distribution of G143A and G1105S in Brazilian field populations. P. viticola isolates were sampled from two vineyards reporting failures of QoI and CAA control. Their growth was unaffected by azoxystrobin and mandipropamid, and resistance was associated with the G143A and G1105S mutations, respectively. The SNP detection pyrosequencing assay developed for G143A detection was precise and accurate. Pyrosequencing assays revealed that G143A and G1105S frequencies varied among the 66 field populations and were positively correlated with the number of QoI and CAA applications, respectively. The G143A mutation was observed in 60 populations, 39 of them with frequencies over 90%. G1105S was detected in 20 populations with frequencies ranging from 18.3% to 100%, and which also carried the G143A mutation. Multiple resistance to QoI and CAA in P. viticola was confirmed for the first time in South America. Future resistance monitoring surveys and management strategies must be adopted to prevent further increase in QoI and CAA resistance in Brazil.

show abstract

“…Advances in P. viticola -omics (14)(15)(16)(17)(18)(19) and population genetic studies (20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35) have improved our knowledge of the grapevine downy mildew pathosystem. In its native range, five cryptic species (also called formae speciales) have recently been identified in the P. viticola species complex, with genetic differentiation and contrasting host ranges on various Vitis and related species (33,34).…”

Section: Introductionmentioning

confidence: 99%

Europe as a bridgehead in the worldwide invasion history of grapevine downy mildew,Plasmopara viticola

Fontaine

Labbé

Dussert

et al. 2020

Preprint

View full text Add to dashboard Cite

Europe is the historical cradle of viticulture, but grapevines have been increasingly threatened by pathogens of American origin. The invasive oomycete Plasmopara viticola causes downy mildew, one of the most devastating grapevine diseases worldwide. Despite major economic consequences, its invasion history remains poorly understood. Comprehensive population genetic analyses of ~2000 samples from the most important wine-producing countries revealed very low genetic diversity in invasive downy mildew populations worldwide. All the populations originated from one of five native North American lineages, the one parasitizing wild summer grape. After an initial introduction into Europe, invasive European populations served as a secondary source of introduction into vineyards worldwide, including China, South Africa and, twice independently, Australia. Invasion of Argentina probably represents a tertiary introduction from Australia. Our findings provide a striking example of a global pathogen invasion resulting from secondary dispersal of a successful invasive population. It will help designing quarantine regulations and efficient breeding for resistance against grapevine downy mildew.

show abstract

Cryptic species and population genetic structure of Plasmopara viticola in São Paulo State, Brazil

Cited by 14 publications

References 28 publications

The Climate-Driven Genetic Diversity Has a Higher Impact on the Population Structure of Plasmopara viticola Than the Production System or QoI Fungicide Sensitivity in Subtropical Brazil

The Climate-Driven Genetic Diversity Has a Higher Impact on the Population Structure of Plasmopara viticola Than the Production System or QoI Fungicide Sensitivity in Subtropical Brazil

Multiple resistance of Plasmopara viticola to QoI and CAA fungicides in Brazil

Europe as a bridgehead in the worldwide invasion history of grapevine downy mildew,Plasmopara viticola

Contact Info

Product

Resources

About