Cryptic subspecies and beauvericin production by Fusarium subglutinans from Europe

Moretti, Antonio; Mulé, Giuseppina; Ritieni, Alberto; Láday, M.; Stubnya, V.; Hornok, L.; Logrieco, A.

doi:10.1016/j.ijfoodmicro.2008.08.003

Cited by 34 publications

(35 citation statements)

References 24 publications

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“…In addition, the F. temperatum:F. subglutinans ratio (about 30:1) was very high in fields, suggesting that F. temperatum outcompetes its sister species F. subglutinans. This information supports the climatic hypothesis of Moretti et al (2008), who observed that in Europe, F. subglutinans tended to occur in warmer and drier regions compared to the majority of strains that are now re-identified as F. temperatum in moderate to cool wet regions. Indeed, most of the non-European F. temperatum strains have been collected in temperate regions of South Africa, USA, Mexico or Guatemala (Scauflaire et al 2011a).…”

Section: Introductionsupporting

confidence: 88%

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Fusarium temperatum, a mycotoxin-producing pathogen of maize

et al. 2012

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In a recent study, a population of Fusarium strains isolated from maize in Belgium was described as a new species, F. temperatum, that is morphologically similar and phylogenetically closely related to F. subglutinans, a species in the American clade of the Gibberella fujikuroi species complex. In fields, the F. temperatum:F. subglutinans ratio was very high, suggesting that F. temperatum outcompetes its sister species F. subglutinans. This raised the question whether this novel species contributes to the final rot symptoms observed on maize plants at harvest, as well as to the potential mycotoxin contamination. Results of the pathogenicity tests by soil and toothpick inoculation demonstrate the ability of F. temperatum to cause seedling malformation and stalk rot under greenhouse conditions. Screening of 15 Fusarium mycotoxins showed the ability of F. temperatum to produce moniliformin, beauvericin, enniatins and fumonisin B 1 .The results indicate that F. temperatum can produce mycotoxins and cause maize diseases and, therefore, poses a potential risk to maize production and to the safety of human food and animal feed.

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Section: Introductionsupporting

confidence: 88%

“…This organism corresponds to the previously described Group 1 of F. subglutinans (Steenkamp et al 2002;Desjardins et al 2006;Moretti et al 2008). Consequently, we propose that the name F. subglutinans strictly circumscribes the second group, namely F. subglutinans Group 2, as applied hereunder.…”

Section: Introductionsupporting

confidence: 51%

Fusarium temperatum, a mycotoxin-producing pathogen of maize

et al. 2012

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“…These are all mountain regions with a warm climate. Previous reports suggested that F. temperatum prefers warmer regions compared to F. subglutinans (Fumero et al, 2015;Moretti et al, 2008;Scauflaire et al, 2011). However, in this study, we also collected F. temperatum in Jilin, northern China, where the annual average temperatures are 3~5 °C and to our knowledge this is the coldest region where F. temperatum was isolated so far.…”

Section: Discussionmentioning

confidence: 84%

Crops are a main driver for species diversity and the toxigenic potential ofFusarium isolates in maize ears in China

Zhang

Brankovics

Luo

et al. 2016

WMJ

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In recent years increasing demands and the relatively low-care cultivation of the crop have resulted in an enormous expansion of the acreage of maize in China. However, particularly in China, Fusarium ear rot forms an important constraint to maize production. In this study, we showed that members of both the Fusarium fujikuroi species complex (FFSC) and the Fusarium graminearum species complex are the causal agents of Fusarium ear rot in the main maize producing areas in China. Fumonisin producing Fusarium verticillioides was the most prevalent species, followed by fumonisin producing Fusarium proliferatum and 15-acetyldeoxynivalenol producing F. graminearum. Both Fusarium temperatum and Fusarium boothii were identified for the first time in the colder regions in China, extending their known habitats to colder environments. Mating type analysis of the different heterothallic FFSC species, showed that both types co-occur in each sampling site suggestive of the possibility of sexual recombination. Virulence tests with F. boothii (from maize) and F. graminearum from maize or wheat showed adaptation to the host. In addition, F. graminearum seems to outcompete F. boothii in wheat-maize rotations. Based on our findings and previous studies, we conclude that wheat/maize rotation selects for F. graminearum, while a wheat/rice rotation selects for F. asiaticum. In contrast, F. boothii is selected when maize is cultivated without rotation. A higher occurrence of F. temperatum is observed on maize in colder climatological regions in China, while Fusarium meridionale seems restricted to mountain areas. Each of these species has their characteristic mycotoxin profile and deoxynivalenol and fumonisin are the potential threats to maize production in Northern China.

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“…subglutinans (Moretti et al, 2008). Very interestingly, this fumonisin production pattern is supporting the hypothesis of a ST1-4 separate lineage within the FLASC though this is not congruent with the multi-gene based phylogeny.…”

Section: Discussionmentioning

confidence: 65%

“…Recommended maximum levels for fumonisins in products intended for animal feed vary between 5 mg/kg and 60 mg/kg. Few studies exist on other mycotoxins such as beauvericin (Jestoi, 2008;Moretti et al, 2008;Yli-Mattila, 2010). Beauvericin is an insecticidal compound, isolated from Beauveria bassiana (Bals.-Criv.)…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity and mycotoxin production of Fusarium lactis species complex isolates from sweet pepper

Poucke¹,

Monbaliu

Munaut

et al. 2012

International Journal of Food Microbiology

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An internal fruit rot disease of sweet peppers was first detected in Belgium in 2003. Research conducted mostly in Canada indicates that this disease is primarily caused by Fusarium lactis Pirotta. Ninety-eight Fusarium isolates obtained from diseased sweet peppers from Belgium, as well as from other countries (Canada, the Netherlands and the United Kingdom) were identified by sequencing the translation elongation factor 1α (EF).Of these 98 isolates, 13 were identified as F. oxysporum Schltdl., nine as F. proliferatum (Matsush.) Nirenberg and two belonged to clade 3 of the F. solani species complex. Of the 74 remaining isolates, the EF sequence showed 97 to 98% similarity to F. lactis. Of these isolates, the β-tubulin (TUB), calmodulin (CAM) and the second largest subunit of RNA polymerase II (RPB2) genes were also sequenced. Analysis of the combined sequences revealed that the 74 isolates share nine combined sequences that correspond to nine multilocus sequence types (STs), while the F. lactis neotype strain and one other strain, both isolated from figs, form a separate ST. Together, these 10 STs represent a monophyletic F. lactis species complex (FLASC).An unusually high level of genetic diversity was observed between (groups of) these STs. Two of them (ST5 and ST6) fulfilled the criteria for species recognition based on genealogical exclusivity and together represent a new monophyletic species lineage (FLASC-1). The seven other STs, together with the F. lactis neotype ST, form a paraphyletic species lineage in the African clade of the Gibberella fujikuroi species complex (GFSC). From each of the 10 STs, the mycotoxin production was assessed using a multi-mycotoxin liquid chromatography mass spectrometry method. Out of the 27 analyzed mycotoxins, beauvericin and fumonisins were detected in sweet pepper tissue and in maize kernels. The 10 STs clearly differed in the amount of mycotoxin produced, but there was only limited congruence between the production profile and the phylogenetic analysis. Furthermore, the morphological characterization (based on mycelial growth rate and the length of macroconidia) showed distinct differences between the 10 STs, but again there was limited congruence with the phylogenetic results.In conclusion, the data presented in this study demonstrate that 75% of the isolates obtained from sweet pepper with internal fruit rot belong to a F. lactis species complex (FLASC), including a new FLASC-1 monophyletic species, and that the members of this complex display great genetic and phenotypic diversity.

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Cryptic subspecies and beauvericin production by Fusarium subglutinans from Europe

Cited by 34 publications

References 24 publications

Fusarium temperatum, a mycotoxin-producing pathogen of maize

Fusarium temperatum, a mycotoxin-producing pathogen of maize

Crops are a main driver for species diversity and the toxigenic potential ofFusarium isolates in maize ears in China

Genetic diversity and mycotoxin production of Fusarium lactis species complex isolates from sweet pepper

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