Cryptotanshinone Protects Cartilage against Developing Osteoarthritis through the miR-106a-5p/GLIS3 Axis

Ji, Quanbo; Qi, Dengbin; Xu, Xiaojie; Xu, Yameng; Goodman, Stuart B.; Liu, Kang; Song, Qi; Zhang, Fan; Maloney, William J.; Wang, Yan

doi:10.1016/j.omtn.2018.02.001

Cited by 25 publications

(21 citation statements)

References 51 publications

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“…Chondrocytes are solely responsible for the synthesis of ECM components 5 , 58 . However, cartilage cellularity is reduced in OA, and the remaining chondrocytes are activated by various cytokines to express a catabolic and abnormal phenotype with poor proliferation and migration potential, resulting in accelerated degradation and limited capacity of the ECM for self-repair and regeneration 3, 4, 5 , 21 .…”

Section: Discussionmentioning

confidence: 99%

miR-140 Attenuates the Progression of Early-Stage Osteoarthritis by Retarding Chondrocyte Senescence

Yang

et al. 2020

Molecular Therapy - Nucleic Acids

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Osteoarthritis (OA) is a major cause of joint pain and disability, and chondrocyte senescence is a key pathological process in OA and may be a target of new therapeutics. MicroRNA-140 (miR-140) plays a protective role in OA, but little is known about its epigenetic effect on chondrocyte senescence. In this study, we first validated the features of chondrocyte senescence characterized by increased cell cycle arrest in the G0/G1 phase and the expression of senescence-associated β-galactosidase (SA-βGal), p16INK4a, p21, p53, and γH2AX in human knee OA. Then, we revealed in interleukin 1β (IL-1β)-induced OA chondrocytes in vitro that pretransfection with miR-140 effectively inhibited the expression of SA-βGal, p16INK4a, p21, p53, and γH2AX. Furthermore, in vivo results from trauma-induced early-stage OA rats showed that intra-articularly injected miR-140 could rapidly reach the chondrocyte cytoplasm and induce molecular changes similar to the in vitro results, resulting in a noticeable alleviation of OA progression. Finally, bioinformatics analysis predicted the potential targets of miR-140 and a mechanistic network by which miR-140 regulates chondrocyte senescence. Collectively, miR-140 can effectively attenuate the progression of early-stage OA by retarding chondrocyte senescence, contributing new evidence of the involvement of miR-mediated epigenetic regulation of chondrocyte senescence in OA pathogenesis.

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Section: Discussionmentioning

confidence: 99%

miR-140 Attenuates the Progression of Early-Stage Osteoarthritis by Retarding Chondrocyte Senescence

Yang

et al. 2020

Molecular Therapy - Nucleic Acids

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“…MiR dysregulation has been implicated in different diseases (Farooqi et al, 2019; Lu, Weng, Li, Yang, & Qian, 2020). Administration of CT upregulates the expression of miR‐106a‐5p to inhibit GLI‐similar 3 (GLIS3), resulting in amelioration of osteoarthritis (Ji et al, 2018). Dual modulation of NF‐κB and Nrf2 signaling pathways lead to excellent anti‐inflammatory activity of CT (Li et al, 2020).…”

Section: Cryptotanshinone: An Overview Of Its Therapeutic Activitiesmentioning

confidence: 99%

“…Administration of CT upregulates the expression of miR-106a-5p to inhibit GLI-similar 3 (GLIS3), resulting in amelioration of osteoarthritis (Ji et al, 2018). Dual modulation of NF-κB and Nrf2 signaling pathways lead to excellent anti-inflammatory activity of CT .…”

Section: Effect On Molecular Pathwaysmentioning

confidence: 99%

Recent advances and future directions in anti‐tumor activity of cryptotanshinone: A mechanistic review

Ashrafizadeh

Zarrabi

Orouei

et al. 2020

Phytotherapy Research

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In respect to the enhanced incidence rate of cancer worldwide, studies have focused on cancer therapy using novel strategies. Chemotherapy is a common strategy in cancer therapy, but its adverse effects and chemoresistance have limited its efficacy. So, attempts have been directed towards minimally invasive cancer therapy using plant derived‐natural compounds. Cryptotanshinone (CT) is a component of salvia miltiorrihiza Bunge, well‐known as Danshen and has a variety of therapeutic and biological activities such as antioxidant, anti‐inflammatory, anti‐diabetic and neuroprotective. Recently, studies have focused on anti‐tumor activity of CT against different cancers. Notably, this herbal compound is efficient in cancer therapy by targeting various molecular signaling pathways. In the present review, we mechanistically describe the anti‐tumor activity of CT with an emphasis on molecular signaling pathways. Then, we evaluate the potential of CT in cancer immunotherapy and enhancing the efficacy of chemotherapy by sensitizing cancer cells into anti‐tumor activity of chemotherapeutic agents, and elevating accumulation of anti‐tumor drugs in cancer cells. Finally, we mention strategies to enhance the anti‐tumor activity of CT, for instance, using nanoparticles to provide targeted drug delivery.

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“…As a highly prevalent disease, osteoarthritis is a considerable burden to public health. Despite several decades of investigation, there is still no effective therapeutic agents for OA treatment [39]. Our study demonstrated that HMGB1 can exert dual effects on inflammatory reaction based on its locations in chondrocytes.…”

Section: Discussionmentioning

confidence: 64%

Dual regulatory roles of HMGB1 in inflammatory reaction of chondrocyte cells and mice

Wenzhao

Song

et al. 2019

Cell Cycle

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Osteoarthritis (OA) is one of the most common bone diseasesas it is reported that the impact of knee osteoarthritis symptomatic form is estimated at 240/100,000 people per year. The inflammation of articular cartilageis thought to be the pathologic drive for development of this disease. HMGB1(high mobility group box-1), a regulatory factor for gene transcription, could stimulate inflammation response. However, theexact regulatory role of HMGB1 in the inflammation of articular cartilage still need to be elucidated. In the current study, we used Quantitative Real-Time PCR(Q-PCR) to detect them RNA levels of Collagen Type II Alpha 1(Col2a1), Aggrecan, MMP3 (Matrix Metallopeptidase 3), MMP13, ADAMTs4 and ADAMTs5; Enzyme-Linked Immunosorbent Assay(ELISA) was used to detect the content of IL-1β and calpain protein; Cell apoptosis was evaluated by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) assay and flow cytometryanalysis; Western blot and immunofluorescence assays were applied to assess the expression of HMGB1; Lastly autophagic activity was mainly verified by monodansylcadaverine (MDC) staining. Our data revealed that in the early stage of chondrocyte inflammation(3 and 6 h of LPS stimulation), cytosolic HMGB1 attenuated inflammation response by facilitating cell autophagy and preventing cell apoptosis. While in the late stage (24 and 48 h of LPS stimulation), the extracellular HMGB1 stimulated inflammation reaction and contributed to the cartilage destruction in OA.

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Cryptotanshinone Protects Cartilage against Developing Osteoarthritis through the miR-106a-5p/GLIS3 Axis

Cited by 25 publications

References 51 publications

miR-140 Attenuates the Progression of Early-Stage Osteoarthritis by Retarding Chondrocyte Senescence

miR-140 Attenuates the Progression of Early-Stage Osteoarthritis by Retarding Chondrocyte Senescence

Recent advances and future directions in anti‐tumor activity of cryptotanshinone: A mechanistic review

Dual regulatory roles of HMGB1 in inflammatory reaction of chondrocyte cells and mice

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