Crystal-Seeded Growth of pH-Responsive Metal–Organic Frameworks for Enhancing Encapsulation, Stability, and Bioactivity of Hydrophobicity Compounds

Abstract

Zeolitic imidazolate framework-L (ZIF-L) could effectively improve the stability, controlled release and anticancer activity of natural hydrophobicity drugs in drug delivery systems (DDS). A simple and universal strategy was developed to prepare the curcumin-loaded ZIF-L

Evaluating the effect of synthesis, isolation, and characterisation variables on reported particle size and dispersity of drug loaded PLGA nanoparticles

“…[43][44][45][46]65 Although there is extensive literature on the use of curcumin either as a therapeutic compound or a drug model, there are several limitations regarding its encapsulation and release studies. [65][66][67][68][69][70][71] Considering the challenges of chemical instability and poor solubility in water, we have chosen curcumin as a challenging drug model to investigate the variables involved in the fabrication of PLGA NPs that might affect drug encapsulation. Specifically, we have investigated the effect of the polymer type and energy input on curcumin encapsulation as well as different methods for determination of the drug encapsulation.…”

Calcium phosphate coated core-shell protein nanocarriers: Robust stability, controlled release and enhanced anticancer activity for curcumin delivery

“…The various samples used and constructed in this study (NaCas, Cur@NaCas and (Cur@NaCas)@CaP) were investigated using transmission emission microscopy (TEM, Hitachi HT7700, Tokyo, Japan) and scanning electron microscope (SEM, Hitachi S-4700, Tokyo, Japan). [35] The average size and zeta-potential of free Cur and the various inclusion complexes were ascertained using dynamic light scattering (DLS, Nano-ZS 2000, Malvern Instruments, UK). The crystalline characterization of free Cur and the various inclusion complexes was facilitated using X-ray diffractometer (XRD, D8 ADVANCE, Germany, Bruker) at a diffraction angle of 20 ranging from 5° to 50° with a stepwise scan rate of 10°/min at 0.2° per step.…”

“…Cellular antioxidant activity (CAA)was determined according to the method reported previously. [35] Briefly, MGC803 cells were seeded in 96-well plates with a concentration of 2.5 x 10 5 cells per milliliter and incubated for 24 h. Then the growth medium was removed and replaced with either 100 µL fresh medium containing 2 µg/mL free Cur or Cur-loaded NPs and incubated for another 24 h. After 24h, fresh medium (100 µL) containing 25 µM DCFH-DA was added into each well and incubated for 1 h. Then, all wells were rinsed with PBS before 100 µL fresh medium f CA represent the integral area of the blank group fluorescence value-time curve.…”

“…According to the principle of CAA, peroxy radicals produced by ABAP cause oxidation of DCFH to form the fluorescent DCF, the DCF fluorescence intensity reflects the degree of oxidation damage caused by free radicals. [35] The antioxidant capacity of any natural compounds can be reflected by the reduced DCF fluorescence intensity. The results (Figure 6A) indicated that DCF accumulated continuously in the cells over time, while Cur could quench free radicals to reduce the concentration of intracellular DCF.…”

Self-Assembled pH-Responsive Metal-Organic Frameworks for Enhancing the Encapsulation and Anti-Oxidation and Melanogenesis Inhibition Activities of Glabridin

“…But this scheme is limited to those drug molecules, such as doxorubicin, camptothecin, and caffeine, that have specific functional groups such as -NH 3 , -COOH, and -SO 3 H [29][30][31]. To overcome these limitations, we tried to use a seed growth method to achieve encapsulation of glabridin by ZIF-8, which has been successfully applied for encapsulation of hydrophobicity compounds [32].…”