Evaluating the effect of synthesis, isolation, and characterisation variables on reported particle size and dispersity of drug loaded PLGA nanoparticles

Garms, Bruna Cambraia; Poli, Hamish; Baggley, Darcy; Han, Yuxiao; Whittaker, Andrew K.; Anitha, A; Grøndahl, Lisbeth

doi:10.1039/d1ma00410g

Cited by 20 publications

(18 citation statements)

References 69 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Variables during PLGA NPs synthesis, isolation (washing and filtration), and characterization could affect the particle size and loading efficiency. Synthesis parameters include the chosen emulsification technique, the type of PLGA polymer and used stabilizer (21) . Overall, all the variables must be chosen carefully and are listed in details.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Peptide nucleic acid delivered by nanoparticles as a gene editing tool: Methods of design, preparation and evaluation

Eltaweel

Amr

El-Kamah

et al. 2022

Azhar International Journal of Pharmaceutical and Medical Scien

View full text Add to dashboard Cite

Synthetic nucleic acids could be designed in sequence-specific structure to trigger endogenous repair systems. Depending on this concept, peptide nucleic acids (or PNAs) could achieve non-enzymatic gene editing i.e. without the use of nucleases unlike CRISPR and TALENs. PNAs could bind to the DNA to form highly specific hetero-triplex structures, so PNAs have been used severally in the last decades to induce correction of different human disease-causing mutations with low off-targets. Systemic in-vivo and in-utero application of PNAs had been enabled thanks to the advances in their chemical structure, design and delivery resulting in considerable preclinical editing in mouse models. Treated animal models engineered with a human beta globin gene (HBB gene carrying a β-thalassemia mutation, showed clinically considerable protein expression, suggesting that PNAs could have a curative potential for genetic disorders. The complete methods of design, preparation and evaluation of the PNA/DNA nanoparticles formulation will be discussed in details in addition to its use in cells treatment aiming for paving the road for interested scientists in gene editing techniques for treating different single gene disorders.

show abstract

Section: Resultsmentioning

confidence: 99%

“…There are several methods of PLGA NPs preparation techniques including emulsion solvent evaporation (ESE), spray-drying and nanoprecipitation. The PLGA NPs formulation technique is chosen according to its application, in addition to the physico-chemical properties of the encapsulated substance (21) .…”

Section: Resultsmentioning

confidence: 99%

Peptide nucleic acid delivered by nanoparticles as a gene editing tool: Methods of design, preparation and evaluation

Eltaweel

Amr

El-Kamah

et al. 2022

Azhar International Journal of Pharmaceutical and Medical Scien

View full text Add to dashboard Cite

show abstract

“…A standard curve was obtained using the different concentrations (2–20 µg/mL) measured at 245 nm. The EE and DL of AMG present in NPs were calculated using the following Equations (3) and (4) [ 43 , 44 ]:

…”

Section: Methodsmentioning

confidence: 99%

Cytotoxicity Enhancement in MCF-7 Breast Cancer Cells with Depolymerized Chitosan Delivery of α-Mangostin

et al. 2022

View full text Add to dashboard Cite

The application of α-mangostin (AMG) in breast cancer research has wide intentions. Chitosan-based nanoparticles (CSNPs) have attractive prospects for developing anticancer drugs, especially in their high flexibility for modification to enhance their anticancer action. This research aimed to study the impact of depolymerized chitosan (CS) on the cytotoxicity enhancement of AMG in MCF-7 breast cancer cells. CSNPs effectivity depends on size, shape, crystallinity degree, and charge surface. Modifying CS molecular weight (MW) is expected to influence CSNPs’ characteristics, impacting size, shape, crystallinity degree, and charge surface. CSNPs are developed using the method of ionic gelation with sodium tripolyphosphate (TPP) as a crosslinker and spray pyrolysis procedure. Nanoparticles’ (NPs) sizes vary from 205.3 ± 81 nm to 450.9 ± 235 nm, ZP charges range from +10.56 mV to +51.56 mV, and entrapment efficiency from 85.35% to 90.45%. The morphology of NPs are all the same spherical forms. In vitro release studies confirmed that AMG–Chitosan–High Molecular Weight (AMG–CS–HMW) and AMG–Chitosan–Low Molecular Weight (AMG–CS–LMW) had a sustained-release system profile. MW has a great influence on surface, drug release, and cytotoxicity enhancement of AMG in CSNPs to MCF-7 cancer cells. The preparations AMG–CS–HMW and AMG–CS–LMW NPs considerably enhanced the cytotoxicity of MCF-7 cells with IC50 values of 5.90 ± 0.08 µg/mL and 4.90 ± 0.16 µg/mL, respectively, as compared with the non-nano particle formulation with an IC50 of 8.47 ± 0.29 µg/mL. These findings suggest that CSNPs can enhance the physicochemical characteristics and cytotoxicity of AMG in breast cancer treatment.

show abstract

“…PLGA nanoparticles were prepared by an emulsion solvent evaporation technique as previously published. 40 Briefly, 10 mg PLGA was dissolved in 0.5 mL DCM (20 mg mL À1 ). This solution was added dropwise to a surfactant solution containing 1.0% w/v PVA (20 mg) in 2 mL of DI water whilst stirring.…”

Section: Materials Fabricationmentioning

confidence: 99%

Evaluation of techniques used for visualisation of hydrogel morphology and determination of pore size distributions

et al. 2023

Self Cite

View full text Add to dashboard Cite

show abstract

Evaluating the effect of synthesis, isolation, and characterisation variables on reported particle size and dispersity of drug loaded PLGA nanoparticles

Abstract: This work provides advice for PLGA-based nanoparticle fabrication and drug encapsulation quantification as well as the minimum required information to be reported allowing reproducibility.

Cited by 20 publications

References 69 publications

Peptide nucleic acid delivered by nanoparticles as a gene editing tool: Methods of design, preparation and evaluation

Peptide nucleic acid delivered by nanoparticles as a gene editing tool: Methods of design, preparation and evaluation

Cytotoxicity Enhancement in MCF-7 Breast Cancer Cells with Depolymerized Chitosan Delivery of α-Mangostin

Evaluation of techniques used for visualisation of hydrogel morphology and determination of pore size distributions

Contact Info

Product

Resources

About