Crystal structure of banana lectin reveals a novel second sugar binding site

Specificity determinants for Cry insecticidal proteins: Insights from their mode of action

“…Domain II presents the highest diversity (suggestive of a role in specificity), and is composed of three antiparallel beta sheets arranged in a beta prism, displaying structural similarities with lectins in the jacalin family (Burton et al, 1999;Xu et al, 2014). In these lectins, three loops protruding from the beta prism structure determine specificity for carbohydrate binding (Meagher et al, 2005). Similar protruding loops in domain II have been shown to be involved in determination of binding specificity to host midgut proteins (Dean et al, 1996;Pigott et al, 2008), although their potential role in recognizing glycan moieties has not been experimentally tested.…”

A Lectin Isolated from Bananas Is a Potent Inhibitor of HIV Replication

“…Lectins in this family are characterized by the presence of a ␤-prism 1 structure composed of three Greek Key turn motifs. Greek Keys 1 and 2 are both involved in binding carbohydrates and contain a GXXXD binding motif, whereas Key 3 does not contain the binding motif (25,26). However, this loop can assist ligand binding and determine lectin specificity (27).…”

Cloning and Expression of a Mannose-Binding Jacalin-Related Lectin from Leaves of Japanese Cycad (Cycas revolutaThunb.)

“…24) In the complex with laminaribiose, a few additional residues were necessary for binding. 25) The same loop, GDVVD, and glycine corresponding to G60 were identified in both domains of CRLL, but were not found in any other JRLs. CRLL 1) and rice-JRL 5) as well as banana lectin 6) recognize laminaribiose, and thus differ from many other JRLs.…”

“…The three-dimensional structures of some mJRLs (Heltuba, 19) Artocarpin, 20,21) Calsepa, 22) MornigaM, 23) and banana lectin 24,25) ) have been reported. In Heltuba, six amino acid residues (G18, M92, G135, D136, V137, and D139) were involved in the binding of mannose residue.…”

“…Recently, a second sugar-binding site was reported for banana lectin. 24,25) In the complex of banana lectin with methyl--D-mannoside, G34, D35, V36, D38, and G60 were involved in sugar-binding. 24) In the complex with laminaribiose, a few additional residues were necessary for binding.…”