Crystalline brucine l-guluronate

Incorporation of isotope from specifically labeled glucose into alginates of Pseudomonas aeruginosa and Azotobacter vinelandii

“…Brucine guluronate hemihydrate was obtained as described previously for brucine mannuronate (15), except that brucine guluronate was precipitated from solution by the addition of a 20to 25-fold excess of acetone. The precipitate was collected, dried, and recrystallized as described previously (17). The melting point of brucine guluronate was 156 to 157°C; the reported melting point is 157 to 159°C (17).…”

“…The precipitate was collected, dried, and recrystallized as described previously (17). The melting point of brucine guluronate was 156 to 157°C; the reported melting point is 157 to 159°C (17). Samples of the purified uronic acids were either dried at 65°C or lyophilized to remove acetic acid, reconstituted with 1 ml of distilled water and 10 ml of Aquasol-2 (New England Nuclear Corp., Boston, Mass.…”