Crystalline ribosomes are present in brains from senile humans.

O'Brien, Liz; Shelley, Kirk H.; Towfighi, Javad; McPherson, A.

doi:10.1073/pnas.77.4.2260

Cited by 45 publications

(13 citation statements)

References 33 publications

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“…2A) anti-MAP-2 (Chemicon, Temecula, California) (panel B), anti-PGP9.5 (Chemicon) (panel C), or antineurofilament antibodies (Sigma) (panel D). The results showed that RSV may infect cells containing Nissl substance (12) (Fig. 2A), microtubule-associated protein (MAP-2; Fig.…”

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confidence: 99%

Respiratory Syncytial Virus (RSV) Infects Neuronal Cells and Processes That Innervate the Lung by a Process Involving RSV G Protein

Li¹,

Alvarez

et al. 2006

J Virol

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Respiratory syncytial virus (RSV) is a primary cause of morbidity and life-threatening lower respiratory tract disease in infants and young children. Children with acute RSV bronchiolitis often develop respiratory sequelae, but the disease mechanisms are poorly understood. Mounting evidence suggests that RSV may mediate persistent infection. Using immunohistochemistry to identify RSV and RSV-infected cell types, we show that RSV infects primary neurons and neuronal processes that innervate the lungs through a process that involves RSV G protein and the G protein CX3C motif. These findings suggest a mechanism for disease chronicity and have important implications for RSV disease intervention strategies.

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confidence: 99%

Respiratory Syncytial Virus (RSV) Infects Neuronal Cells and Processes That Innervate the Lung by a Process Involving RSV G Protein

Li¹,

Alvarez

et al. 2006

J Virol

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“…Moreover, it is not unreasonable to believe that during prolonged culture, some of the E. coli cells undergo 'aging' analogous to eukaryotes. In fact, the preservation of ribosomes in association with membranes has recently been reported in human brains exhibiting senile and pre-senile dementias [29]. This suggests that at least some of the mechanisms of ribosome turnover may be universal in prokaryotes and eukaryotes.…”

Section: Discussionmentioning

confidence: 90%

Metabolic changes in ribosomes of Escherichia coli during prolonged culture in different media

Ramagopal

1984

Eur J Biochem

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The metabolism of ribosomes during the exponential growth and post-exponential phase of Escherichia coli cells was investigated. Incubation of E. coli cells in two rich media: L-broth and phosphate medium, up to stationary phase shows no drop in viability or any changes in ribosomes. However, the survival rate during prolonged culture of the post-stationary-phase cells has been found to be a function of the incubation medium. The decline in viability is only slight in phosphate medium but very rapid in L-broth. So long as the viability is maintained, the level of ribosomes and the relative abundance of rRNA and ribosomal proteins in ribosomes of the post-stationary cultures are remarkably stable and are similar to exponentially growing cells. On the other hand, post-stationary cultures undergoing a rapid drop in cell viability lose 95 % of the original ribosomes. These cultures accumulate a large pool of 30s and 50s subunits and a few 70s monosomes, all of which show deficiency in the various ribosomal proteins. No differences in rRNA can be detected but the number and the relative stoichiometry of individual ribosomal proteins are drastically altered. Only 13 of the 53 proteins known in the E. coli ribosome appeared in the same relative amounts as in the ribosomes of the exponentially growing cells. Six proteins (S12, S21, L2, L16, L20, L34) are completely lost and all others undergo partial loss. An analysis of the number and relative abundance of ribosomal proteins in the whole cells, as oppossed to isolated ribosomes, suggests that during the initial stages of the catabolism of ribosomes a crucial step is the formation of ribosomal-subunit-membrane complexes. The data emphasize the role of the constituents of ribosomes not only for the growth but also for the survival of E. colicells. A model for the metabolism of ribosomes during the exponential growth and post-exponential phase of E. coli is presented.The metabolism of exponentially growing bacterial cells has been very extensively studied. In contrast, our knowledge of the post-exponential-phase bacterial cell is still quite limited. Bacteria such as Escherichia colidivide exponentially only for a short period in nature and reach the stationary phase. Stationary-phase cells may be fully viable for some time but the viability begins to drop after a certain period. Cells that survive in the stationary phase and during the phase of decline in viability probably do so on endogenous resources or by utilization of substances released from the dead cells. Indeed, metabolites derived from the death of 50 cells of Klebsiella aerogenes were found to support the doubling of one individual in glycerol-limited cultures [ 11. Other studies have suggested that the maintenance of a critical level of adenylate energy charge [2] and carbon, nitrogen and phosphorus from degradation of glycogen, poly(3-hydroxybutyrate), polyphosphates and free amino acids [3] were important for cell survival.Not much is known of the roles of macromolecules, such as RNA and protein, in cell ...

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“…The presence of HBs in DLB patients has not been previously reported. FAC1 represents a novel protein component of HBs to be included in an expanding list of HB‐associated proteins containing actin, actin‐associated proteins, APP, and possibly ribosomes [ 7, 17].…”

Section: Discussionmentioning

confidence: 99%

The presence of FAC1 protein in Hirano bodies

Jordan‐Sciutto

Dragich

Walcott

et al. 1998

Neuropathology Appl Neurobio

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We have previously reported that the FAC1 protein is contained in hippocampal structures that resemble Hirano bodies. Hirano bodies are cytoplasmic inclusions containing actin filaments that are numerous in the hippocampus of many Alzheimer's disease patients. FAC1 is a developmentally regulated protein that is localized to the cytoplasm of neurons during development and is predominately a nuclear protein in adult brain. In hippocampal sections from non-demented adults. Alzheimer's disease, and dementia with Lewy bodies patients. Hirano bodies were immunolabelled with antibodies to the FAC1 protein. Confocal laser microscopy demonstrated the presence of actin in FAC1 labelled Hirano bodies, and ultrastructural analysis confirmed the presence of a lattice structure within FAC1 labelled Hirano bodies. Numerous FAC1 immunoreactive swollen dendrites were also present in the hippocampus of Alzheimer's disease and dementia with Lewy bodies patients. Within any one case the total number of FAC1 positive swollen dendrites correlated with the total number of Hirano bodies, suggesting an association between the two structures. Thus, FAC1 protein is contained in Hirano bodies and swollen dendrites in the hippocampus of patients with Alzheimer's disease and dementia with Lewy bodies.

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Crystalline ribosomes are present in brains from senile humans.

Cited by 45 publications

References 33 publications

Respiratory Syncytial Virus (RSV) Infects Neuronal Cells and Processes That Innervate the Lung by a Process Involving RSV G Protein

Respiratory Syncytial Virus (RSV) Infects Neuronal Cells and Processes That Innervate the Lung by a Process Involving RSV G Protein

Metabolic changes in ribosomes of Escherichia coli during prolonged culture in different media

The presence of FAC1 protein in Hirano bodies

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