Crystalline Vitamin B12 in Pharmaceutical Preparations

Macek, Thomas J.; Feller, Beate A.

doi:10.1002/jps.3030410602

Cited by 11 publications

(9 citation statements)

References 3 publications

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“…However, vitamin B12 is quite stable to dry heat. Macek and Feller ( 1955) found only a slight loss on heating at 120° C for 30 min. Shenoy and Ramasarma ( 1955) reported that the stability of the vitamin to prolonged heating depends on the inorganic milieu.…”

Section: Methodsmentioning

confidence: 93%

Relative Amounts of Vitamin B "12" in Detritus from Oceanic and Estuarine Environments Near Sapelo Island, Georgia

Starr¹

1956

Ecology

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Section: Methodsmentioning

confidence: 93%

Relative Amounts of Vitamin B "12" in Detritus from Oceanic and Estuarine Environments Near Sapelo Island, Georgia

Starr¹

1956

Ecology

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“…. Dilutions of Byl in water are buffered at p H 4.5-5.0, the region of opti-ma1 stability; citrate buffer is recommended ( 15). It should be noted that this is not the procedure routinely used by the British author; see Part 2 of this paper.…”

Section: Euglena Gracilismentioning

confidence: 99%

A Sugar‐Containing Basal Medium for Vitamin B₁₂‐Assay with Euglena; Application to Body Fluids*

Hutner

Bach

Ross³

1956

The Journal of Protozoology

415

113

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SUMMARY. An improved assay of vitamin B12 is described. The z strain of Euglena gracilis, which grows more vigorously than the bacillaris strain previously used, is recommended. The pattern of B12 specificity of the two strains appears to be the same. A new medium containing sucrose, aspartic acid, glutamic acid, and glycine at pH 3.6 is well buffered and allows luxuriant growth. Vigorous utilization of sugar appears to depend on readily available nitrogen and a Krebs‐cycle component; these requirements are well met by aspartic acid (or asparagine) and glutamic acid. The proposed procedure is especially suitable for the measurement of B12 in blood serum because rise in pH and precipitation of serum proteins during incubation are minimized. Like bacillaris, the z strain allows the distinction between “combined” and “uncombined” B12 in serum. Serum may be an appropriate test material to tell whether such phagotrophs as Peranema can better utilize bound forms of vitamins than can the related osmotrophs. Patterns of B12 requirements and occurrence are discussed as phylogenetic markers.

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“…There would be a fourth type of solution, i. e., B complex solutions coritaining vitamin BIZ and vitamin C. The well known and confirmed incompatibility of vitamin BIZ with vitamin C (1,3,5,6,7,21) leads us not to include this type in our study.…”

Section: Type B Solutionsmentioning

confidence: 99%

The Combination of B-Complex Vitamins and Ascorbic Acid in Aquedus Solutions**Research Department of Laborterapica S. A., Industrie Quimica e Farmactutica, Sao Paulo, Brazil

Gambier¹,

Rahn²

1957

Journal of the American Pharmaceutical Association (Scientific

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Crystalline Vitamin B12 in Pharmaceutical Preparations

Cited by 11 publications

References 3 publications

Relative Amounts of Vitamin B "12" in Detritus from Oceanic and Estuarine Environments Near Sapelo Island, Georgia

Relative Amounts of Vitamin B "12" in Detritus from Oceanic and Estuarine Environments Near Sapelo Island, Georgia

A Sugar‐Containing Basal Medium for Vitamin B₁₂‐Assay with Euglena; Application to Body Fluids*

The Combination of B-Complex Vitamins and Ascorbic Acid in Aquedus Solutions**Research Department of Laborterapica S. A., Industrie Quimica e Farmactutica, Sao Paulo, Brazil

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Crystalline Vitamin B12 in Pharmaceutical Preparations

Cited by 11 publications

References 3 publications

Relative Amounts of Vitamin B "12" in Detritus from Oceanic and Estuarine Environments Near Sapelo Island, Georgia

Relative Amounts of Vitamin B "12" in Detritus from Oceanic and Estuarine Environments Near Sapelo Island, Georgia

A Sugar‐Containing Basal Medium for Vitamin B12‐Assay with Euglena; Application to Body Fluids*

The Combination of B-Complex Vitamins and Ascorbic Acid in Aquedus Solutions**Research Department of Laborterapica S. A., Industrie Quimica e Farmactutica, Sao Paulo, Brazil

Contact Info

Product

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A Sugar‐Containing Basal Medium for Vitamin B₁₂‐Assay with Euglena; Application to Body Fluids*