Crystallization of an Endopolygalacturonase from Acrocylindrium sp

Uchino, Fuji; Kurono, Yoshiaki; Doi, Shinji

doi:10.1271/bbb1961.30.1066

Cited by 3 publications

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“…The enzyme was purified by a method based on that reported by Uchino et al (1966), as follows. After 72 h cultivation, mycelia were removed by centrifugation at Ioooog for 5 min.…”

Section: E T H 0 D Smentioning

confidence: 99%

“…In our laboratory, Uchino et al (1966) found that a strain of Acrocylindriurn produced a significant amount of polygalacturonase. The enzyme was crystallized and called endopolygalacturonase because it was not able to hydrolyse more than 50% of the glycosidic bonds in polygalacturonic acid.…”

Section: Introductionmentioning

confidence: 97%

“…Such polygalacturonases have been classified into two groups. One group which hydrolyses internal bonds of polygalacturonic acid has been found from fungi (Mill & Tuttobello, 1961;Endo, 1964a;Uchino, Kurono & Doi, 1966;Koller & Neukom, 1969), from a bacterium (Nasuno & Starr, 1966), from a yeast (Patel & Phaff, 1959) and from tomato (Patel & Phaff, 1960). According to Demain & Phaff's (1957) definition, these enzymes are called endo-polygalacturonases.…”

Section: Introductionmentioning

confidence: 99%

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Properties of a Polygalacturonase Produced by Acrocylindrium

Kimura

Uchino

Mizushima

1973

Journal of General Microbiology

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S U M M A R YA polygalacturonase has been purified from the culture fluid of a strain of Acrocylindrium. The purified preparation was homogeneous when examined by ultracentrifugation. The hydrolysis of di-and trigalacturonic acids by the enzyme was undetectable. Tetragalacturonic acid was hydrolysed to tri-and monogalacturonic acids, while pentagalacturonic acid was hydrolysed to either tri-and digalacturonic acids or tetra-and inonogalacturonic acids. The rate of hydrolysis of pentagalacturonic acid to tri-and digalacturonic acids was about four times as fast as that to tetra-and monogalacturonic acids. It was concluded that the enzyme can hydrolyse any glycosidic bond between the reducing end and the third galacturonic-acid unit from the non-reducing end of polygalacturonic acid. The manner of hydrolysis of polygalacturonic acid by the enzyme is different from that of any other polygalacturonase reported so far.

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“…The enzyme was purified by a method based on that reported by Uchino et al (1966), as follows. After 72 h cultivation, mycelia were removed by centrifugation at Ioooog for 5 min.…”

Section: E T H 0 D Smentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%