Three strains of acido-thermophilic bacteria were isolated from hot-spring waters of Tohoku district in Japan. They were aerobic spore-forming bacilli and identified to belong to genus Bacillus. Their characteristics were as follows. They were acidophilic, and grew well in the pH range of between 2.3 and 5.0. Optimal growth conditions were 65°C for temperature and 3.5~4.0 for pH of media. Strains T-4 and T-17 required biotin as growth factor, but T-7 did not require any factors for its growth. These bacteria were different from Bacillus stearothermophilus or B. coagulans in their taxonomic properties.

Three strains of acido-thermophilic bacteria were isolated from hot-spring waters of Tohoku district in Japan. They were aerobic spore-forming bacilli and identified to belong to genus Bacillus. Their characteristics were as follows. They were acidophilic , and grew well in the pH range of between 2.3 and 5.0. Optimal growth conditions were 65°C for temperature and 3.54.0 for pH of media. Strains T-4 and T-17 required biotin as growth factor, but T-7 did not require any factors for its growth. These bacteria were different from Bacillus stearothermophilus or B. coagulans in their taxonomic properties.

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A crystalline acid protease produced by a strain of Acrocylindrium in a submerged culture was prepared by treatment with acetone (60%), salting out with ammonium sulfate (saturated) and, after chromatography on Duolite CS-101 column, dialysis against distilled water. This preparation was homogeneous on sedimentation analysis, starch-gel electro phoresis and gel filtration with Sephadex G-75. The optimum pH was 2.0 for milk casein digestion and the pH stability was for 2.0•`5.0 at 30•Ž for one day. The crystalline enzyme was completely stable below 50•Ž, but lost the activity at 70•Ž in ten minutes. The acid protease was almost equal to pepsin on specific activity when milk casein solution (pH 2.0) was used as substrate. Purification and Some Properties of Crystalline Acid Protease from Acrocylindrium sp .