CsMYB184 regulates caffeine biosynthesis in tea plants

Li, Penghui; Ye, Zhili; Fu, Jiamin; Xu, Yujie; Shen, Yihua; Zhang, Yanrui; Tang, Dingkun; Li, Ping; Zuo, Hao; Tong, Wei; Wang, Shucai; Fernie, Alisdair R.; Zhao, Jian

doi:10.1111/pbi.13814

Cited by 27 publications

(26 citation statements)

References 5 publications

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“…The expression of many later genes involved in catechin biosynthesis showed a similar expression pattern in tea leaves and flowers. In tea plants, at least 10 MYB transcription factors regulated catechin biosynthesis together with WDR type TF TRANSPARENT TESTA GLABRA1 (TTG1) and bHLH type TF TRANSPARENT TESTA8 (TT8), in which the MYB184 has been identified as the key regulator regulating EGCG and ECG biosynthesis in tea leaves ( Li et al., 2022a ; Li et al., 2022c ). The correlation analysis showed that the expression of MYB184 and other MYB TFs had a significant correlation with the expression of ANR and LAR , two key catechin biosynthetic genes both in developing tea leaves and developing flowers, which indicated that catechin biosynthesis regulation is conservative in different tissues.…”

Section: Discussionmentioning

confidence: 99%

Integrating metabolite and transcriptome analysis revealed the different mechanisms of characteristic compound biosynthesis and transcriptional regulation in tea flowers

Tang

Shen

et al. 2022

Front. Plant Sci.

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The flowers of tea plants (Camellia sinensis), as well as tea leaves, contain abundant secondary metabolites and are big potential resources for the extraction of bioactive compounds or preparation of functional foods. However, little is known about the biosynthesis and transcriptional regulation mechanisms of those metabolites in tea flowers, such as terpenoid, flavonol, catechins, caffeine, and theanine. This study finely integrated target and nontarget metabolism analyses to explore the metabolic feature of developing tea flowers. Tea flowers accumulated more abundant terpenoid compounds than young leaves. The transcriptome data of developing flowers and leaves showed that a higher expression level of later genes of terpenoid biosynthesis pathway, such as Terpene synthases gene family, in tea flowers was the candidate reason of the more abundant terpenoid compounds than in tea leaves. Differently, even though flavonol and catechin profiling between tea flowers and leaves was similar, the gene family members of flavonoid biosynthesis were selectively expressed by tea flowers and tea leaves. Transcriptome and phylogenetic analyses indicated that the regulatory mechanism of flavonol biosynthesis was perhaps different between tea flowers and leaves. However, the regulatory mechanism of catechin biosynthesis was perhaps similar between tea flowers and leaves. This study not only provides a global vision of metabolism and transcriptome in tea flowers but also uncovered the different mechanisms of biosynthesis and transcriptional regulation of those important compounds.

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Section: Discussionmentioning

confidence: 99%

Integrating metabolite and transcriptome analysis revealed the different mechanisms of characteristic compound biosynthesis and transcriptional regulation in tea flowers

Tang

Shen

et al. 2022

Front. Plant Sci.

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“…Furthermore, multiple MYB binding sites were found in the promoter of TCS1 ( Figure 6 B). In tea plants, CsMYB184 (TEA029017/CSS0026328) were proved to positively regulate caffeine biosynthesis [ 22 ]. However, the irrelevance was found between the expression level of CsMYB184 and the accumulation of caffeine in this study ( Table S4 ).…”

Section: Discussionmentioning

confidence: 99%

“…As the first N -methyltransferase gene, TCS1 has been supposed to be the key enzyme which catalyzes the methylation of N -3 and N -1 [ 21 ]. Furthermore, an MYB family gene ( CsMYB184 ) has been proved to regulate caffeine biosynthesis in tea plants by the function of TCS1 promoter activation [ 22 ]. To meet the personalized needs of different groups, breeding of the new germplasm with abundant catechins and low caffeine has been one of the goals for genetic improvement.…”

Section: Introductionmentioning

confidence: 99%

Metabolomic and Transcriptomic Analyses Reveal the Characteristics of Tea Flavonoids and Caffeine Accumulation and Regulation between Chinese Varieties (Camellia sinensis var. sinensis) and Assam Varieties (C. sinensis var. assamica)

Tang

Zhang

Liu

et al. 2022

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Flavonoids and caffeine are the major secondary metabolites with beneficial bioactivity for human health in tea plants, and their biosynthesis pathway and regulatory networks have been well-deciphered. However, the accumulation traits of flavonoids and caffeine in different tea cultivars was insufficient in investigation. In this study, metabolomic and transcriptomic analyses were performed to investigate the differences of flavonoids and caffeine accumulation and regulation between Chinese varieties, including the ‘BTSC’ group with green leaf, the ‘BTZY’ group with purple foliage, and the ‘MYC’ group comprising Assam varieties with green leaf. The results showed that most of the flavonoids were down-regulated in the ‘MYC’ group; however, the total anthocyanin contents were higher than that of the ‘BTSC’ group while lower than that of the ‘BTZY’ group. An ANS (Anthocyanin synthase) was significantly up-regulated and supposed to play a key role for anthocyanin accumulation in the ‘BTZY’ group. In addition, the results showed that esterified catechins were accumulated in the ‘BTSC’ and ‘BTZY’ groups with high abundance. In addition, SCPL1A (Type 1A serine carboxypeptidase-like acyltransferases gene) and UGGT (UDP glucose: galloyl-1-O-β-d-glucosyltransferase gene) potentially contributed to the up-accumulation of catechins esterified by gallic acid. Interestingly, the results found that much lower levels of caffeine accumulation were observed in the ‘MYC’ group. RT-qPCR analysis suggested that the expression deficiency of TCS1 (Tea caffeine synthase 1) was the key factor resulting in the insufficient accumulation of caffeine in the ‘MYC’ group. Multiple MYB/MYB-like elements were discovered in the promoter region of TCS1 and most of the MYB genes were found preferentially expressed in ‘MYC’ groups, indicating some of which potentially served as negative factor(s) for biosynthesis of caffeine in tea plants. The present study uncovers the characteristics of metabolite accumulation and the key regulatory network, which provide a research reference to the selection and breeding of tea varieties.

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“…Tea [Camellia sinensis (L.) O. Kuntze] is an important leaf-consumed economic plant in China; healthy leaves have significant impacts on the quality of tea beverages. Tea leaves are rich in polyphenols, caffeine, theanine, and other secondary metabolites, which confer teas with rich taste, pleasant flavors, and multiple health benefits. − The diverse flavonoid derivatives in tea plants, such as catechins, anthocyanins, and proanthocyanidins (PAs), also appear to function in resistance against various biotic and abiotic stresses, such as low temperature, UV irradiation, and fungal infection. , When tea trees suffered from extreme temperatures or UV-B irradiation, the increased accumulation of anthocyanins is one of the most common phenotypic traits. , The accumulation of lignin in tea plants often changes with the occurrence of biological stress . The ability of tea tree to resist pests and diseases depends on the rich natural polyphenols on the one hand, and, on the other hand, plant hormones such as SA and methyl jasmonate (MeJA) also play a vital role in the process of resisting pathogenic bacteria.…”

Section: Introductionmentioning

confidence: 99%

Transcriptional Analysis of Tea Plants (Camellia sinensis) in Response to Salicylic Acid Treatment

Liu

Wang

et al. 2023

J. Agric. Food Chem.

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Salicylic acid (SA) is an important plant hormone and signal required for establishing resistance to diverse pathogens and plant diseases. The abundant polyphenols in tea plants also defend plants from biotic and abiotic stresses. However, whether exogenous SA would increase the resistance of tea plants to adversity and the relationship between SA and polyphenols are still poorly understood. Here, we carried out SA treatment on tea seedlings and performed transcriptome sequencing. SA treatment inhibited the phenylpropanoid and flavonoid metabolic pathways but promoted the lignin metabolic pathways. The increased accumulation of lignin in tea leaves after treating with SA indicated that lignin might coordinate SA, enhance, and improve plant defense and disease resistance. Simultaneously, an SA-inducible flavonoid glucosyltransferase (CsUGT0554) specifically involved in 7-OH site glycosylation was characterized in vitro. These results provided valuable information about the effects of SA on tea seedlings and the molecular basis for SA-mediated immune responses.

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CsMYB184 regulates caffeine biosynthesis in tea plants

Cited by 27 publications

References 5 publications

Integrating metabolite and transcriptome analysis revealed the different mechanisms of characteristic compound biosynthesis and transcriptional regulation in tea flowers

Integrating metabolite and transcriptome analysis revealed the different mechanisms of characteristic compound biosynthesis and transcriptional regulation in tea flowers

Metabolomic and Transcriptomic Analyses Reveal the Characteristics of Tea Flavonoids and Caffeine Accumulation and Regulation between Chinese Varieties (Camellia sinensis var. sinensis) and Assam Varieties (C. sinensis var. assamica)

Transcriptional Analysis of Tea Plants (Camellia sinensis) in Response to Salicylic Acid Treatment

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