CsRCI2A and CsRCI2E genes show opposite salt sensitivity reaction due to membrane potential control

Kim, Hyunsung; Lee, Jieun; Jang, Ha-Young; Kwak, Kyungjin; Ahn, Sung‐Ju

doi:10.1007/s11738-016-2072-3

Cited by 19 publications

(30 citation statements)

References 62 publications

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“…Based on the various physiological characteristics of these members, the TOM genes were found to have low molecular weights ranging from 10.049 kDa to 44.279 kDa, with all their GRAVY values of less than one an indication that these proteins were hydrophobic in nature [ 67 ]. The identity of TOM genes and the hydrophobic nature is coherent with a number of salt stress tolerance genes such as CsRCI2A and CsRCI2E , which were isolated from Camelina sativa (L.) (a biodiesel crop with high tolerance to cold stress); the two genes were found to be highly induced due to salinity stress [ 68 ].…”

Section: Discussionmentioning

confidence: 99%

A Novel G-Protein-Coupled Receptors Gene from Upland Cotton Enhances Salt Stress Tolerance in Transgenic Arabidopsis

Magwanga

et al. 2018

Genes

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Plants have developed a number of survival strategies which are significant for enhancing their adaptation to various biotic and abiotic stress factors. At the transcriptome level, G-protein-coupled receptors (GPCRs) are of great significance, enabling the plants to detect a wide range of endogenous and exogenous signals which are employed by the plants in regulating various responses in development and adaptation. In this research work, we carried out genome-wide analysis of target of Myb1 (TOM1), a member of the GPCR gene family. The functional role of TOM1 in salt stress tolerance was studied using a transgenic Arabidopsis plants over-expressing the gene. By the use of the functional domain PF06454, we obtained 16 TOM genes members in Gossypium hirsutum, 9 in Gossypium arboreum, and 11 in Gossypium raimondii. The genes had varying physiochemical properties, and it is significant to note that all the grand average of hydropathy (GRAVY) values were less than one, indicating that all are hydrophobic in nature. In all the genes analysed here, both the exonic and intronic regions were found. The expression level of Gh_A07G0747 (GhTOM) was significantly high in the transgenic lines as compared to the wild type; a similar trend in expression was observed in all the salt-related genes tested in this study. The study in epidermal cells confirmed the localization of the protein coded by the gene TOM1 in the plasma membrane. Analysis of anti-oxidant enzymes showed higher concentrations of antioxidants in transgenic lines and relatively lower levels of oxidant substances such as H2O2. The low malondialdehyde (MDA) level in transgenic lines indicated that the transgenic lines had relatively low level of oxidative damage compared to the wild types. The results obtained indicate that Gh_A07G0747 (GhTOM) can be a putative target gene for enhancing salt stress tolerance in plants and could be exploited in the future for the development of salt stress-tolerant cotton cultivars.

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Section: Discussionmentioning

confidence: 99%

A Novel G-Protein-Coupled Receptors Gene from Upland Cotton Enhances Salt Stress Tolerance in Transgenic Arabidopsis

Magwanga

et al. 2018

Genes

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“…These include Arabidopsis ( Capel et al, 1997 ; Medina et al, 2001 , 2007 ; Nylander et al, 2001 ), maize ( Fu et al, 2012 ; Zhao et al, 2014 ), barley ( Goddard et al, 1993 ; Ueda et al, 2004 ), alfalfa and barrel medic ( Long et al, 2015 ), wheatgrass ( Gulick and Dvořák, 1992 ; Galvez et al, 1993 ), strawberry ( NDong et al, 1997 ), Physcomitrella patens ( Kroemer et al, 2004 ), sheepgrass ( Inada et al, 2005 ), rice ( Morsy et al, 2005 ; Kim et al, 2007 ; Chang-Qing et al, 2008 ), wheat ( Imai et al, 2005 ), alkali grass ( Chang-Qing et al, 2008 ), mandarin ( Gimeno et al, 2009 ), plantain ( Feng et al, 2009 ; Liu et al, 2012 ), red sage ( Wang D. et al, 2013 ), smooth cordgrass ( Baisakh et al, 2008 ; Baisakh and Subudhi, 2009 ), and Aulacomnium turgidum ( Liu et al, 2010 ). Additionally, the RCI2/PMP3 gene expression profiles have been updated for camelina ( Kim et al, 2016 , 2020 ), pearl millet ( Yeshvekar et al, 2017 ), and cucumber ( Zhou et al, 2020 ).…”

Section: Changes To Rci2 Expression In Response To Abiotic Stressesmentioning

confidence: 99%

“…The rare cold inducible 2 (RCI2s) are putative membrane proteins that are considerably induced by environmental stresses, such as salt, cold, and drought, in most plant species, microorganisms, and nematodes, but not in higher animals ( Medina et al, 2007 ; Kim et al, 2016 ; Rocha, 2016 ). RCI2 names, such as low temperature inducible 6 (LTI6), plasma membrane proteolipid 3 (PMP3), and sensitive to Na + (SNA) can vary depending on the species.…”

Section: Introductionmentioning

confidence: 99%

“…The protein sequence alignment and phylogenetic tree for RCI2s clearly showed that they were divided by their structural properties ( Supplementary Figure 1 ). Additionally, several studies have reported that the over-expression of RCI2 proteins which do not contain the C-terminal hydrophilic tail contributed physiological changes and enhance abiotic stress tolerance ( Feng et al, 2009 ; Kim et al, 2016 , 2019 ). However, little is known about the function of tail-type RCI2 proteins when plants are subjected to cold, drought, and salt stress.…”

Section: Introductionmentioning

confidence: 99%

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Subcellular Journey of Rare Cold Inducible 2 Protein in Plant Under Stressful Condition

Kim

Park²,

Lee

et al. 2021

Front. Plant Sci.

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Rare cold inducible 2 (RCI2) proteins are small hydrophobic membrane proteins in plants, and it has been widely reported that RCI2 expressions are dramatically induced by salt, cold, and drought stresses in many species. The RCI2 proteins have been shown to regulate plasma membrane (PM) potential and enhance abiotic stress tolerance when over-expressed in plants. RCI2 protein structures contain two transmembrane domains that are thought to be PM intrinsic proteins and have been observed at the PM and endomembranes. However, cellular trafficking of RCI2s are not fully understood. In this review, we discussed (i) general properties of RCI2s characterized in many species, (ii) the uses of RCI2s as a tracer in live cell imaging analyses and when they are fused to fluorescence proteins during investigations into vesicle trafficking, and (iii) RCI2 functionalities such as their involvement in rapid diffusion, endocytosis, and protein interactions. Consequently, the connection between physiological characteristics of RCI2s and traffic of RCI2s interacting membrane proteins might be helpful to understand role of RCI2s contributing abiotic stresses tolerance.

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“…Samples were respectively incubated with 10 μl of DiBAC 4 (3) dye and DiOC 2 (3) dye for 10 min. DiBAC 4 (3) dye strength was measured by the enzyme-labeled instrument (Kim, Lee, Jang, Kwak, & Ahn, 2016). Flow cytometry was performed in the wild-type strain and strain Y03 that obtained by screening the most tolerant strain among the module-assembly strains via DiOC 2 (3) dye (Tan et al, 2017).…”

mentioning

confidence: 99%

Engineering of membrane phospholipid component enhances salt stress tolerance in Saccharomyces cerevisiae

Yin

Zhu

Luo

et al. 2020

Biotech & Bioengineering

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To increase the growth of industrial strains under environmental stress, the Saccharomyces cerevisiae BY4741 salt-tolerant strain Y00 that tolerates 1.2 M NaCl was cultured through nitroguanidine mutagenesis. The metabolomics and transcription data of Y00 were compared with those of the wild-type strain BY4741. The comparison identified two genes related to salt stress tolerance, cds1 and cho1. Modular assembly of cds1 and cho1 redistributed the membrane phospholipid component and decreased the ratio of anionic-to-zwitterionic phospholipid in strain Y03 that showed the highest salt tolerance. Therefore, significantly increased membrane potential and membrane integrity helped strain Y03 to resist salt stress (1.2 M NaCl). This study provides an effective membrane engineering strategy to enhance salt stress tolerance.

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CsRCI2A and CsRCI2E genes show opposite salt sensitivity reaction due to membrane potential control

Cited by 19 publications

References 62 publications

A Novel G-Protein-Coupled Receptors Gene from Upland Cotton Enhances Salt Stress Tolerance in Transgenic Arabidopsis

A Novel G-Protein-Coupled Receptors Gene from Upland Cotton Enhances Salt Stress Tolerance in Transgenic Arabidopsis

Subcellular Journey of Rare Cold Inducible 2 Protein in Plant Under Stressful Condition

Engineering of membrane phospholipid component enhances salt stress tolerance in Saccharomyces cerevisiae

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