CsWRKY25 Improves Resistance of Citrus Fruit to Penicillium digitatum via Modulating Reactive Oxygen Species Production

Wang, Wenjun; Li, Ting; Chen, Qi; Yao, Shixiang; Deng, Lili; Zeng, Kaifang

doi:10.3389/fpls.2021.818198

Cited by 8 publications

(3 citation statements)

References 57 publications

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“…In Dioscorea composita , the expression of DcWRKY3 (Group I) is strongly affected by salt stress ( Yu et al, 2022 ). The transcription level of CsWRKY25 (Group I) is up-regulated in Penicillium digitatum -infected citrus peels ( Wang et al, 2022b ). PcWRKY11 (Group II WRKY from Polygonum cuspidatum ) significantly increases the tolerance to salt stress in transgenic A. thaliana ( Wang et al, 2022a ).…”

Section: Discussionmentioning

confidence: 99%

Genome-wide identification of Cymbidium sinense WRKY gene family and the importance of its Group III members in response to abiotic stress

Wei

Jin²,

Gao³

et al. 2022

Front. Plant Sci.

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Transcription factors (TFs) of the WRKY family play pivotal roles in defense responses and secondary metabolism of plants. Although WRKY TFs are well documented in numerous plant species, no study has performed a genome-wide investigation of the WRKY gene family in Cymbidium sinense. In the present work, we found 64 C. sinense WRKY (CsWRKY) TFs, and they were further divided into eight subgroups. Chromosomal distribution of CsWRKYs revealed that the majority of these genes were localized on 16 chromosomes, especially on Chromosome 2. Syntenic analysis implied that 13 (20.31%) genes were derived from segmental duplication events, and 17 orthologous gene pairs were identified between Arabidopsis thaliana WRKY (AtWRKY) and CsWRKY genes. Moreover, 55 of the 64 CsWRKYs were detectable in different plant tissues in response to exposure to plant hormones. Among them, Group III members were strongly induced in response to various hormone treatments, indicating their potential essential roles in hormone signaling. We subsequently analyzed the function of CsWRKY18 in Group III. The CsWRKY18 was localized in the nucleus. The constitutive expression of CsWRKY18 in Arabidopsis led to enhanced sensitivity to ABA-mediated seed germination and root growth and elevated plant tolerance to abiotic stress within the ABA-dependent pathway. Overall, our study represented the first genome-wide characterization and functional analysis of WRKY TFs in C. sinense, which could provide useful clues about the evolution and functional description of CsWRKY genes.

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Section: Discussionmentioning

confidence: 99%

Genome-wide identification of Cymbidium sinense WRKY gene family and the importance of its Group III members in response to abiotic stress

Wei

Jin²,

Gao³

et al. 2022

Front. Plant Sci.

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“…In addition to phenylpropanoid biosynthetic genes, transcriptional factors such as MYB, bHLH, ERF, and WRKY are also associated with the regulation of flavonoid biosynthesis and/or disease resistance in plants ( Liu et al., 2021 ; Naik et al., 2022 ). WRKY transcription has been implicated in the regulation of flavonoid biosynthesis ( Naik et al., 2022 ), and also acts as a transcriptional activator that participates in citrus disease resistance against P. digitatum ( Wang et al., 2021 ; Wang et al., 2022 ). MYC, also known as the bHLH transcription factor, activates genes that participate in plant secondary metabolite biosynthesis and exhibits beneficial effects on plant growth via resisting fungal pathogens ( Teng et al., 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Comparative transcriptomic and metabolomic analyses reveal the delaying effect of naringin on postharvest decay in citrus fruit

Zeng

Chen

et al. 2022

Front. Plant Sci.

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IntroductionNaringin exhibits antioxidant capacity and can partially inhibit pathogens in many horticultural products, such as citrus fruit; however, the effects of naringin on the storage quality and mechanisms that regulate senescence in citrus fruit have not been comprehensively analyzed.Methods and resultsIn this study, exogenous naringin treatment was found to significantly delay citrus fruit disease, decreasing the H2O2 content, increasing the antioxidant capacity and maintaining the quality of the fruit. Metabolomic analysis of citrus peel indicated the vast majority (325) of metabolites belonging to flavonoids. Moreover, the auraptene, butin, naringenin, and luteolin derivative levels within the phenylpropanoid pathway were significantly higher in the naringin-treated fruit than in the control fruit. Transcriptomic analysis also revealed that twelve genes in the phenylpropanoid and flavonoid biosynthesis pathways were significantly upregulated. Further analysis with a co-expression network revealed significant correlation between these differential genes and metabolites. Additionally, MYC and WRKY, screened from the MAPK signaling pathway, may contribute to naringin-induced disease resistance.ConclusionIn conclusion, naringin treatment can efficiently delay decay and maintain the quality of citrus fruit, mainly by promoting metabolites accumulation, and upregulating differentially expressed genes in phenylpropanoid and flavonoid biosynthesis pathway. This study provides a better understanding of the regulatory mechanisms through which naringin delays citrus fruit decay and maintains fruit quality.

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“…WRKY transcription factors regulate plant defense systems through complex defense networks. For example, CsWRKY25 can improve resistance to Penicillium digitatum by regulating ROS production and PR gene expression in citrus [ 16 ]. CaWRKY40 interacts with CaMPK9 to enhance the stability of CaWRKY40 , and CaWRKY40 positively regulated the transcription of CaWRKY33 to improve resistance to F. oxysporum in chickpea [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

Overexpression of CmWRKY8-1–VP64 Fusion Protein Reduces Resistance in Response to Fusarium oxysporum by Modulating the Salicylic Acid Signaling Pathway in Chrysanthemum morifolium

Miao

Wang

et al. 2023

IJMS

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Chrysanthemum Fusarium wilt, caused by the pathogenic fungus Fusarium oxysporum, severely reduces ornamental quality and yields. WRKY transcription factors are extensively involved in regulating disease resistance pathways in a variety of plants; however, it is unclear how members of this family regulate the defense against Fusarium wilt in chrysanthemums. In this study, we characterized the WRKY family gene CmWRKY8-1 from the chrysanthemum cultivar ‘Jinba’, which is localized to the nucleus and has no transcriptional activity. We obtained CmWRKY8-1 transgenic chrysanthemum lines overexpressing the CmWRKY8-1-VP64 fusion protein that showed less resistance to F. oxysporum. Compared to Wild Type (WT) lines, CmWRKY8-1 transgenic lines had lower endogenous salicylic acid (SA) content and expressed levels of SA-related genes. RNA-Seq analysis of the WT and CmWRKY8-1-VP64 transgenic lines revealed some differentially expressed genes (DEGs) involved in the SA signaling pathway, such as PAL, AIM1, NPR1, and EDS1. Based on Gene Ontology (GO) enrichment analysis, the SA-associated pathways were enriched. Our results showed that CmWRKY8-1-VP64 transgenic lines reduced the resistance to F. oxysporum by regulating the expression of genes related to the SA signaling pathway. This study demonstrated the role of CmWRKY8-1 in response to F. oxysporum, which provides a basis for revealing the molecular regulatory mechanism of the WRKY response to F. oxysporum infestation in chrysanthemum.

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CsWRKY25 Improves Resistance of Citrus Fruit to Penicillium digitatum via Modulating Reactive Oxygen Species Production

Cited by 8 publications

References 57 publications

Genome-wide identification of Cymbidium sinense WRKY gene family and the importance of its Group III members in response to abiotic stress

Genome-wide identification of Cymbidium sinense WRKY gene family and the importance of its Group III members in response to abiotic stress

Comparative transcriptomic and metabolomic analyses reveal the delaying effect of naringin on postharvest decay in citrus fruit

Overexpression of CmWRKY8-1–VP64 Fusion Protein Reduces Resistance in Response to Fusarium oxysporum by Modulating the Salicylic Acid Signaling Pathway in Chrysanthemum morifolium

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