Ctr1 transports silver into mammalian cells

Bertinato, Jesse; Cheung, Lawrence H.; Hoque, Rezaul; Plouffe, Louise J.

doi:10.1016/j.jtemb.2010.01.009

Cited by 68 publications

(60 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Moreover, recently, it has been demonstrated that CTR1 is an important Ag transporter. Cells over-expressing Ctr1 accumulated Ag and cells lacking Ctr1 had a 50% reduction in Ag content compared with wild-type [104]. In addition, the evidence that Ag þ inhibit Cu uptake suggests that monovalent Cu is the favourite substrate for CTR1.…”

Section: Molecular Mechanisms Of Ag Uptakementioning

confidence: 99%

“…Both excess and deficiency of Cu is deleterious for the cell, thus Cu intracellular concentration is tightly regulated by Cu transporter proteins [102]. Notably, some of these proteins have been shown to transport Ag as well as Cu [103,104].…”

Section: Molecular Mechanisms Of Ag Uptakementioning

confidence: 99%

“…In Cu uptake studies, inhibition of Cu uptake by Ag is used as a marker of CTR1-mediated transport [108]. However, it should also be mentioned that Ctr1-deficent cells (Ctr1 knock out embryonic cells) still accumulated some Cu or Ag, approximately 30 [113] or 50% [104], respectively, showing that there are alternative Ctr1-independent systems. Ctr1-deficient cells express residual Cu transport activity that saturates and is characterized by a K m for Cu of 10 mM (rather than 1-5 mM of CTR1) [113].…”

Section: Molecular Mechanisms Of Ag Uptakementioning

confidence: 99%

See 2 more Smart Citations

Bioavailability of silver nanoparticles and ions: from a chemical and biochemical perspective

Behra

Sigg

Clift

et al. 2013

J. R. Soc. Interface.

290

184

View full text Add to dashboard Cite

Owing to their antimicrobial properties, silver nanoparticles (NPs) are the most commonly used engineered nanomaterial for use in a wide array of consumer and medical applications. Many discussions are currently ongoing as to whether or not exposure of silver NPs to the ecosystem (i.e. plants and animals) may be conceived as harmful or not. Metallic silver, if released into the environment, can undergo chemical and biochemical conversion which strongly influence its availability towards any biological system. During this process, in the presence of moisture, silver can be oxidized resulting in the release of silver ions. To date, it is still debatable as to whether any biological impact of nanosized silver is relative to either its size, or to its ionic constitution. The aim of this review therefore is to provide a comprehensive, interdisciplinary overview-for biologists, chemists, toxicologists as well as physicists-regarding the production of silver NPs, its (as well as in their ionic form) chemical and biochemical behaviours towards/within a multitude of relative and realistic biological environments and also how such interactions may be correlated across a plethora of different biological organisms.

show abstract

Section: Molecular Mechanisms Of Ag Uptakementioning

confidence: 99%

Section: Molecular Mechanisms Of Ag Uptakementioning

confidence: 99%

Section: Molecular Mechanisms Of Ag Uptakementioning

confidence: 99%

See 1 more Smart Citation

Bioavailability of silver nanoparticles and ions: from a chemical and biochemical perspective

Behra

Sigg

Clift

et al. 2013

J. R. Soc. Interface.

290

184

View full text Add to dashboard Cite

show abstract

“…It is generally thought that cuprous (Cu ϩ ), rather than cupric (Cu 2ϩ ) copper ions are transported by hCTR1 (40). Of numerous metals tested, only monovalent silver (Ag ϩ ) inhibits or competes with 64 Cu uptake (27), and it was subsequently shown that hCTR1 transports Ag ϩ (8). Furthermore, using spectroscopic techniques, De Feo et al (13) showed that purified recombinant hCTR1 bound two Cu ϩ ions per trimer (13).…”

mentioning

confidence: 99%

Cellular glutathione plays a key role in copper uptake mediated by human copper transporter 1

Maryon

Molloy

Kaplan

2013

American Journal of Physiology-Cell Physiology

159

130

View full text Add to dashboard Cite

show abstract

“…CTR/COPT proteins are highly specific for Cu + transport (and the isoelectric Ag + ), but not for Cu 2+ [13,36,37]. Consequently, they function in coordination with membrane metalloreductases that catalyze Cu 2+ reduction to Cu + before transport [38,39].…”

Section: Introductionmentioning

confidence: 99%

Function and Regulation of the Plant COPT Family of High-Affinity Copper Transport Proteins

Puig

2014

Advances in Botany

View full text Add to dashboard Cite

Copper (Cu) is an essential micronutrient for all eukaryotes because it participates as a redox active cofactor in multiple biological processes, including mitochondrial respiration, photosynthesis, oxidative stress protection, and iron (Fe) transport. In eukaryotic cells, Cu transport toward the cytoplasm is mediated by the conserved CTR/COPT family of high-affinity Cu transport proteins. This outlook paper reviews the contribution of our research group to the characterization of the function played by the Arabidopsis thaliana COPT1-6 family of proteins in plant Cu homeostasis. Our studies indicate that the different tissue specificity, Curegulated expression, and subcellular localization dictate COPT-specialized contribution to plant Cu transport and distribution. By characterizing lack-of-function Arabidopsis mutant lines, we conclude that COPT1 mediates root Cu acquisition, COPT6 facilitates shoot Cu distribution, and COPT5 mobilizes Cu from storage organelles. Furthermore, our work with copt2 mutant and COPToverexpressing plants has also uncovered Cu connections with Fe homeostasis and the circadian clock, respectively. Future studies on the interaction between COPT transporters and other components of the Cu homeostasis network will improve our knowledge of plant Cu acquisition, distribution, regulation, and utilization by Cu-proteins.

show abstract

Ctr1 transports silver into mammalian cells

Cited by 68 publications

References 38 publications

Bioavailability of silver nanoparticles and ions: from a chemical and biochemical perspective

Bioavailability of silver nanoparticles and ions: from a chemical and biochemical perspective

Cellular glutathione plays a key role in copper uptake mediated by human copper transporter 1

Function and Regulation of the Plant COPT Family of High-Affinity Copper Transport Proteins

Contact Info

Product

Resources

About