CTX-M-producing Escherichia coli Isolated from urban pigeons (Columba livia domestica) in Brazil

Cunha, Marcos Paulo Vieira; Oliveira, M. C.; Oliveira, Maria Gabriela Xavier de; Menão, Márcia Cristina; Knöbl, Terezinha

doi:10.3855/jidc.11441

Cited by 19 publications

(15 citation statements)

References 26 publications

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“…The carriage of ESBL producers in pigeons in our study was comparable to previous studies around the world, which have reported the detection of E. coli isolates harbouring bla CTX-M genes from pigeons, including Bangladesh, France, Germany, Nicaragua, China, and Brazil [ 9 , 10 , 34 , 35 , 40 , 41 ]. In this study, we also detected the bla OXA-48 gene in E. coli isolates from different urban places around the city, including a sewage treatment plant, a university, and a public park.…”

Section: Discussionsupporting

confidence: 89%

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Detection of blaOXA-48 and mcr-1 Genes in Escherichia coli Isolates from Pigeon (Columba livia) in Algeria

et al. 2022

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The emergence and spread of β-lactams and colistin-resistant Escherichia coli in birds deserve a special concern worldwide. This study aimed to investigate the presence of β-lactams and colistin-resistant Escherichia coli strains isolated from the faeces of urban and rural pigeons in Batna, Algeria, and to characterise their molecular traits of resistance. Between March and April 2019, a total of 276 faecal droppings samples were collected in Batna, Algeria. Samples were subjected to selective isolation of β-lactams and colistin-resistant Escherichia coli. The representative colonies were then identified using Matrix-Assisted Laser Desorption-Ionization Time-of-Flight Mass Spectrometry. Antimicrobial susceptibility testing was performed using the disc diffusion method. β-lactamases, as well as mcr genes, were screened for by PCR and confirmed by sequencing. Genetic relatedness of the mcr-positive E. coli strains was determined using multi-locus sequence typing analysis. Transferability features of carbapenemase genes were assessed by conjugation experiments. Overall, thirty-five E. coli isolates were obtained only from urban pigeon samples. All carbapenem-resistant isolates harboured the blaOXA-48 gene as the only carbapenemase gene detected (n = 11), while blaESBL genes were detected in eighteen isolates. Out of the thirty-five isolates, four E. coli isolates were positive for the mcr-1 gene. The obtained mcr-1 positive E. coli isolates belonged to four STs, including ST1485, ST224, ST46, and a new ST. This study is the first to report the isolation of E. coli strains carrying the mcr-1 gene from pigeon faeces in Algeria and also the first to report the detection of blaOXA-48-positive E. coli in pigeons. Close surveillance is, therefore, urgently needed to monitor the dissemination of blaOXA-48 and mcr-1 producing E. coli strains in wildlife.

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Section: Discussionsupporting

confidence: 89%

“…The presence of multi-drug resistant bacteria in pigeons is generally linked to faecal contamination of both human and animal origin [ 7 ]. Carriage of such bacteria in pigeon faeces has been reported in different countries, with much of the focus being on Escherichia coli species [ 8 , 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

Detection of blaOXA-48 and mcr-1 Genes in Escherichia coli Isolates from Pigeon (Columba livia) in Algeria

et al. 2022

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“…Interestingly, clonal plasmids carrying blaCTX-M genes are largely restricted to animals (6,8,22,24). Moreover, blaCTX-M genes in this group are mainly associated with I1 plasmids (6,8,22,25,26), and not reported from F plasmids which are the dominant plasmid type overall in E. coli. Therefore, blaCTX-M genes of this group may seldom exchange blaCTX-M genes with other plasmids.…”

Section: Methodsmentioning

confidence: 79%

Dissecting the clonality of I1 plasmids using ORF-based binarized structure network analysis of plasmids (OSNAp)

Suzuki

Norizuki

Wachino

et al. 2021

Preprint

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Phylogenetic relationship of 97 I1 plasmids harboring blaCTX-M genes encoding extended-spectrum beta-lactamase (ESBL) was analyzed using the ORF-based binarized structure network analysis of plasmids (OSNAp). The majority of plasmids carrying blaCTX-M-1 or blaCTX-M-8, ESBL genes primarily associated with domestic animals, were clonal. On the other hand, plasmids carrying bla>CTX-M-14 or bla>CTX-M-15, identified from both humans and domestic animals, were diverse in their contents. The findings suggest that circulation of I1 plasmids among humans and animals may contribute to their diversity.

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“…It was previously shown that pigeons are reservoir of multidrug-resistant E. coli , including ESBL-producing strains ( 17 – 19 ). Cunha et al ( 20 ) found that feral pigeons carried ESBL-positive E. coli strains producing the enzymes CTX-M-2 and CTX-M-8 ( 20 ).…”

Section: Discussionmentioning

confidence: 99%

Pigeons as Carriers of Clinically Relevant Multidrug-Resistant Pathogens—A Clinical Case Report and Literature Review

et al. 2021

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Pigeons are widespread bird species in urban regions (Columba livia forma urbana) and may carry pathogens with zoonotic potential. In recent years, more and more data indicate that these zoonotic pathogens are multidrug resistant. Our results confirmed that global trend. Three different multidrug-resistant pathogens were isolated from an oral cavity of a racing pigeon with lesions typical for pigeon pox virus infection. Staphylococcus aureus was recognized as methicillin resistant, thus resistant to all beta-lactams. Additionally, it was also resistant to many other classes of antibiotics, namely: aminoglycosides, tetracyclines, phenicols, lincosamides, and macrolides. Escherichia coli showed resistance to all antimicrobials tested, and it was classified as intermediate to amikacin. Moreover, Candida albicans resistant to clotrimazole, natamycin, flucytosine, and amphotericin and intermediate to ketoconazole, nystatin, and econazole was also isolated. This raises the question how pigeons acquire such highly resistant strains. Therefore, more data are needed concerning the resistance to antibiotics in strains from domestic and wild pigeons in Poland. Until the problem is fully understood, it will be challenging to implement adequate planning of any control measures and check their effectiveness.

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CTX-M-producing Escherichia coli Isolated from urban pigeons (Columba livia domestica) in Brazil

Cited by 19 publications

References 26 publications

Detection of blaOXA-48 and mcr-1 Genes in Escherichia coli Isolates from Pigeon (Columba livia) in Algeria

Detection of blaOXA-48 and mcr-1 Genes in Escherichia coli Isolates from Pigeon (Columba livia) in Algeria

Dissecting the clonality of I1 plasmids using ORF-based binarized structure network analysis of plasmids (OSNAp)

Pigeons as Carriers of Clinically Relevant Multidrug-Resistant Pathogens—A Clinical Case Report and Literature Review

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