CTX-M β-Lactamases inEscherichia colifrom Community-acquired Urinary Tract Infections, Cambodia

Abstract

Despite the recent global spread of CTX-M β-lactamases in Escherichia coli isolates from community-acquired urinary tract infections (CA-UTIs), their dissemination has been little studied in developing countries. In a 2-year prospective study, we documented the prevalence of extended-spectrum β-lactamases (ESBLs) in E. coli that were responsible for CA-UTIs in Phnom-Penh, Cambodia. Ninety-three E. coli strains were included. We observed a high prevalence of resistance to amoxicillin (88.2% of strains), cotrimoxazole (75.3%), ciprofl oxacin (67.7%), gentamicin (42.5%), and third-generation cephalosporins (37.7%). A total of 34 strains carried ESBLs, all of which were CTX-M type. CTX-M carriage was associated with resistance to fl uoroquinolones and aminoglycosides. Using repetitive extragenic palindromic-PCR, we identifi ed 4 clusters containing 9, 8, 3, and 2 strains. The prevalence of CTX-M β-lactamases has reached a critical level in Cambodia, which highlights the need for study of their spread in developing countries.

Surveillance of post-caesarean surgical site infections in a hospital with limited resources, Cambodia

“…In Cambodia, antimicrobial resistance is a growing concern which has already been documented for Neisseria gonorrhoeae [21], S. aureus [22], Salmonella [23,24], Shigella [23], and Escherichia coli [25]. Nearly 45% of the 20 S. aureus strains isolated by the Kampong Cham Hospital laboratory in 2011 were community acquiredmethicillin resistant S. aureus (MRSA) (Somary N., Personal communication, 2011).…”

Antimicrobial susceptibility of uropathogens isolated from Cambodian children

“…5,6 Increasing levels of infection with extended-spectrum b-lactamase (ESBL)-producing E. coli strains have been described in Phnom Penh, the capital of Cambodia, and neighbouring countries. [7][8][9] We report the species and antimicrobial susceptibility of bacteria causing UTIs in children attending a paediatric hospital in north-west Cambodia.…”

Genetic Determinants of Antibiotic Resistance in Hospital and Community Isolates of Klebsiella pneumoniae and Escherichia coli

“…The small number of samples harbored this gene, can result in this finding. Amongst 34 ESBL producing E. coli isolates from community acquired urinary tract infections in Cambodia, all were positive for bla CTX-M and negative for bla VEB and bla TEM in 26 (76.4%) of the ESBL-carrying strains was reported (29). One hundred and thirty seven E. coli (73.6%) were not ESBL producing according to PCT.…”

“…According to a French study, the prevalence of CTX-M-producing E. coli among the total number of E. coli isolates was 60.8%, and the occurrence of CTX-M-producing E. coli among ESBL-producing E. coli isolates was 39.8% (20). A polish study revealed that Most of the ESBL-producing isolates (75.7 %) had a bla CTX-M gene (29). Some studies have indicated that CTX-M-producing E. coli and K. pneumoniae have been imported from community acquired urinary tract infections into the hospital setting (27).…”