Cu, Cd, As and Hg resistance levels in Escherichia coli isolated from Mediterranean mussel and sea snail in the Southeastern Black Sea

Terzi̇, Ertuğrul; Civelek, Fatih

doi:10.33714/masteb.776902

Cited by 9 publications

(5 citation statements)

References 23 publications

(20 reference statements)

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“…Moller et al (2014) reported merA in strains with phenotypic mercury resistance. In another study, mercury-resistant (7.4%) E. coli strains were isolated from mussels and sea snails, and the presence of merA was reported (Terzi and Civelek, 2021).…”

Section: Discussionmentioning

confidence: 99%

Investigation of Some Heavy Metal Resistance Genes in E.coli Isolated from Shrimp and Mussels

Celik,

Sipahi,

Kekec

et al. 2023

J Hellenic Vet Med Soc

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One of the biggest problems in the modern world is environmental pollution. All organisms are affected by the resulting pollution, albeit at different levels. One of the most important causes of pollution is the stable and non-perishable compounds formed due to heavy metals used in a wide range of environments. Heavy metal pollution, especially in the seas, causes cumulative accumulations in aquatic life, food and other products obtained from aquatic life, in living things that consume these products, and related diseases. The most critical sign of heavy metal pollution in water is the resistance formed against heavy metals in bacteria living in these waters. To determine the resistance to heavy metals in bacteria, the presence of resistance to Copper, Mercury and Manganese heavy metals in E. coli isolated from 18 mussels and 16 shrimps was genotypically investigated. For this purpose, the presence of pcoR genes for the determination of copper resistance, merA for the determination of mercury resistance, and mntR for the determination of manganese resistance was investigated in both plasmid and genomic DNA. As a result of the study, resistance genes were detected against heavy metals in 31 (91.17%) of 34 E. coli isolates examined. The presence of the pcoR gene (copper resistance) was found in 2 isolates (5.88%), the merA gene (mercury resistance) in only one isolate (2.94%) and the mntR gene (manganese resistance) in 8 isolates (23.52%). While pcoR and merA genes were not observed together in any isolates, pcoR and mntR genes were detected together in the genetic material of 10 isolates (29.41%). In comparison, mntR and merA genes were detected together in the genetic material of 7 isolates (20.58%). In 3 samples, all of the resistance genes against heavy metals were detected (8.82%).

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Section: Discussionmentioning

confidence: 99%

Investigation of Some Heavy Metal Resistance Genes in E.coli Isolated from Shrimp and Mussels

Celik,

Sipahi,

Kekec

et al. 2023

J Hellenic Vet Med Soc

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“…The ability of Hg to inhibit the growth of these bacteria isolates could be due to its high level of toxicity [48,49]. Contrastingly, most of the bacteria isolated were resistant to high concentration of Cd and Co, in tandem with this is the result of the study of Terzi and Civelek in which they observed that high concentration of Cd was still being tolerated by their bacteria isolates [17]. HMRGs namely merA (mercury), pcoC (copper), pbrT (lead), cadD (cadmium), and cnrA (cobalt and nickel) were genotyped in isolates 2, 6, 7, and 9.…”

Section: Discussionmentioning

confidence: 99%

“…Of note, these resistance mechanisms are often conferred by natural selection or exposure to substrates containing heavy metal ions [16]. Salmonella, Escherichia coli, and Rhodobacter sphaeroides are among the bacteria that have been reported to possess multiple heavy metal resistance genes (HMRGs); hence, they are regarded as suitable for environmental remediation [6,17].…”

Section: Introductionmentioning

confidence: 99%

Physiochemical analyses and molecular characterization of heavy metal-resistant bacteria from Ilesha gold mining sites in Nigeria

Ojo,

Onile,

Momoh

et al. 2023

Journal of Genetic Engineering and Biotechnology

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Background The contribution of the processes involved and waste generated during gold mining to the increment of heavy metals concentration in the environment has been well established. While certain heavy metals are required for the normal functioning of an organism, certain heavy metals have been identified for their deleterious effects on the ecosystem and non-physiological roles in organisms. Hence, efforts aimed at reducing their concentration level are crucial. To this end, soil and water samples were collected from Ilesha gold mining, Osun State, Nigeria, and they were subjected to various analyses aimed at evaluating their various physicochemical parameters, heavy metal concentration, heavy metal-resistant bacteria isolation, and other analyses which culminated in the molecular characterization of heavy metal-resistant bacteria. Results Notably, the results obtained from this study revealed that the concentration of heavy metal in the water samples around the mining site was in the order Co > Zn > Cd > Pb > Hg while that of the soil samples was in the order Co > Cd > Pb > Hg > Zn. A minimum inhibitory concentration test performed on the bacteria isolates from the samples revealed some of the isolates could resist as high as 800 ppm of Co, Cd, and Zn, 400 ppm, and 100 ppm of Pb and Hg respectively. Molecular characterization of the isolates revealed them as Priestia aryabhattai and Enterobacter cloacae. Conclusion Further analysis revealed the presence of heavy metal-resistant genes (HMRGs) including merA, cnrA, and pocC in the isolated Enterobacter cloacae. Ultimately, the bacteria identified in this study are good candidates for bioremediation and merit further investigation in efforts to bioremediate heavy metals in gold mining sites.

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“…The sea snail "Rapa whelk" is a good bioindicator for metals, and its bioaccumulation is highly dependent on the level of metal pollution in the ecosystem (Liang et al 2004;Terzi & Civelek 2021), possibly because of their low motion on the sea floor (Hwang et al 2017). Despite being a seafood with notable health benefits, when Rapa whelk is consumed at high intake levels it can be seriously detrimental to health (Stancheva et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Health risk assessments of metals via consumption of Rapa whelk (Rapana venosa) from the Black Sea

Bayraklı,

Yiğit,

Altuntaş

et al. 2024

J Agr Sci-Tarim Bili

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The present study investigated the bioaccumulation of metals in raw, heat treated -and sterilized Rapa whelk, and evaluated consumer risks for human. All metals, except Mn, were lower than permissible FAO standards. A remarkable amount of metal was released into the cooking juice (Al, Cr, Cu, Fe, Hg, Pb, Sb, Se, Zn) after heat treatment, and hypochlorite solution (Al, As, Cu, Hg, Mn, Pb, Se, Zn). After sterilization, the levels for As, Mo, Cd, Sb, Cr, Zn, Se, Cu, and Hg in Rapa whelk were reduced by 47.4%, 40.1%, 24.9%, 20.3%, 17.5%, 4.5%, 3.6%, 0.93%, and 0.68%, respectively. Metals in Rapa whelk exposed to hypochlorite immersion were below permissible upper limits. Target hazard quotients for non-carcinogenic risks of consuming sterilized Rapa whelk were below “1” (THQ

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Cu, Cd, As and Hg resistance levels in Escherichia coli isolated from Mediterranean mussel and sea snail in the Southeastern Black Sea

Cited by 9 publications

References 23 publications

Investigation of Some Heavy Metal Resistance Genes in E.coli Isolated from Shrimp and Mussels

Investigation of Some Heavy Metal Resistance Genes in E.coli Isolated from Shrimp and Mussels

Physiochemical analyses and molecular characterization of heavy metal-resistant bacteria from Ilesha gold mining sites in Nigeria

Health risk assessments of metals via consumption of Rapa whelk (Rapana venosa) from the Black Sea

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