Cu(II) ion loading in silk fibroin scaffolds with silk I structure

Hua, Jinsheng; You, Haining; Li, Xiufang; You, Renchuan; Ma, Likun

doi:10.1016/j.ijbiomac.2020.04.094

Cited by 22 publications

(9 citation statements)

References 36 publications

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“…So far, many papers, including this one, assigned the silk I structures using IR and Raman spectroscopies by carefully considering type II β-turn model [ 52 , 53 , 100 , 101 , 102 , 103 , 104 , 105 , 106 , 107 , 108 ]. For example, Monti et al [ 52 , 104 ] assigned the strong amide I, II, II, and V bands fell at 1654, 1540, 1240, and 660 cm −1 to type II β-turn structure in the IR spectrum of (AG) 15 with silk I form.…”

Section: Problems In Speculating Silk I Structure From the Ir Spectrummentioning

confidence: 99%

Structure of Silk I (Bombyx mori Silk Fibroin before Spinning) -Type II β-Turn, Not α-Helix-

Asakura

2021

Molecules

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Recently, considerable attention has been paid to Bombyx mori silk fibroin by a range of scientists from polymer chemists to biomaterial researchers because it has excellent physical properties, such as strength, toughness, and biocompatibility. These appealing physical properties originate from the silk fibroin structure, and therefore, structural determinations of silk fibroin before (silk I) and after (silk II) spinning are a key to make wider applications of silk. There are discrepancies about the silk I structural model, i.e., one is type II β-turn structure determined using many solid-state and solution NMR spectroscopies together with selectively stable isotope-labeled model peptides, but another is α-helix or partially α-helix structure speculated using IR and Raman methods. In this review, firstly, the process that led to type II β-turn structure by the authors was introduced in detail. Then the problems in speculating silk I structure by IR and Raman methods were pointed out together with the problem in the assignment of the amide I band in the spectra. It has been emphasized that the conformational analyses of proteins and peptides from IR and Raman studies are not straightforward and should be very careful when the proteins contain β-turn structure using many experimental data by Vass et al., (Chem. Rev., 2003, 103, 1917–1954). In conclusion, the author emphasized here that silk I structure should be type II β-turn, not α-helix.

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Section: Problems In Speculating Silk I Structure From the Ir Spectrummentioning

confidence: 99%

Structure of Silk I (Bombyx mori Silk Fibroin before Spinning) -Type II β-Turn, Not α-Helix-

Asakura

2021

Molecules

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“…1 . Generally, regenerated SF aqueous solution was first prepared by dissolving SF, which was extracted from the B. mori cocoon by the classical protocol [ 26 ]. After the addition of Cu(II) salt, a Cu-SF complex with square planar coordination modes of Cu-2N2O [Cu-SF(I)] was formed due to the chelation between Cu 2+ ions and SF at a pH value of 5.0, whereas a Cu-SF complex with Cu-4N mode [Cu-SF(II)] was formed at a pH of 11.0.…”

Section: Resultsmentioning

confidence: 99%

“…Comparatively, the expression of TNF-α significantly decreased after treatment with CuN 4 -CNS + H 2 O 2 + NIR. Based on this, it is inferred that the CuN 4 -CNS SAzyme can effectively alleviate the inflammatory response under infection conditions, accelerating wound healing and tissue regeneration, which may be due to the promoting effects of copper species in CuN 4 -CNS for cell migration, angiogenesis, and collagen deposition [ 26 ].…”

Section: Resultsmentioning

confidence: 99%

Alternative Copper-Based Single-Atom Nanozyme with Superior Multienzyme Activities and NIR-II Responsiveness to Fight against Deep Tissue Infections

Bai

Feng

et al. 2023

Research

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Nanozymes are considered to represent a new era of antibacterial agents, while their antibacterial efficiency is limited by the increasing tissue depth of infection. To address this issue, here, we report a copper and silk fibroin (Cu-SF) complex strategy to synthesize alternative copper single-atom nanozymes (SAzymes) with atomically dispersed copper sites anchored on ultrathin 2D porous N-doped carbon nanosheets (CuN x -CNS) and tunable N coordination numbers in the CuN x sites ( x = 2 or 4). The CuN x -CNS SAzymes inherently possess triple peroxidase (POD)-, catalase (CAT)-, and oxidase (OXD)-like activities, facilitating the conversion of H 2 O 2 and O 2 into reactive oxygen species (ROS) through parallel POD- and OXD-like or cascaded CAT- and OXD-like reactions. Compared to CuN 2 -CNS, tailoring the N coordination number from 2 to 4 endows the SAzyme (CuN 4 -CNS) with higher multienzyme activities due to its superior electron structure and lower energy barrier. Meanwhile, CuN x -CNS display strong absorption in the second near-infrared (NIR-II) biowindow with deeper tissue penetration, offering NIR-II-responsive enhanced ROS generation and photothermal treatment in deep tissues. The in vitro and in vivo results demonstrate that the optimal CuN 4 -CNS can effectively inhibit multidrug-resistant bacteria and eliminate stubborn biofilms, thus exhibiting high therapeutic efficacy in both superficial skin wound and deep implant-related biofilm infections.

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“…XRD test was also carried out to further confirm the structure difference of silk sample before and after functional modification (Figure 2). The XRD of the control sample showed the characteristic Silk-I configuration at 20.7 and 24.4° [26]. After FR modification, such peaks and structures of silk were also clear, but the peak at 20.7° shifted to 20.4°, and the peak at 24.4° showed increased intensity, indicating the ferric salt modification influenced the β -turn configuration (amide I and II) of silk.…”

Section: Resultsmentioning

confidence: 99%

Hydrophobic and flame-retardant coating on silk via ferric salt chelation

Cheng

Guan

et al. 2021

Surface Engineering

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The present study facilely prepared the hydrophobic and flame-retardant (FR) multifunctional coating on silk textile through ferric ion chelation route, and focused on multifunctional abilities and mechanisms of ferric salt on silk. Morphology and structure properties of the modified silk were characterised by scanning electron microscope, atomic force microscopy and X-ray diffraction analyses. The functional abilities, mechanisms and durability were also studied. The multifunctional silk with self-extinguishing ability (char length: 10.1 cm) and hydrophobic effect (water contact angle (WCA): 126.1°) could be obtained using only 0.25 g L −1 ferric salt. The modified silk still had desirable functionalities after five robust launderings. The hydrophobic ability is mainly dependent on the increased surface roughness. Thermal stability and char residue analyses indicated the condensed phase FR mechanism of functional silk. The present study provides a cleaner and cost-effective process to develop hydrophobic and FR multifunctional coating on silk surface by ferric metal salt modification.

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Cu(II) ion loading in silk fibroin scaffolds with silk I structure

Cited by 22 publications

References 36 publications

Structure of Silk I (Bombyx mori Silk Fibroin before Spinning) -Type II β-Turn, Not α-Helix-

Structure of Silk I (Bombyx mori Silk Fibroin before Spinning) -Type II β-Turn, Not α-Helix-

Alternative Copper-Based Single-Atom Nanozyme with Superior Multienzyme Activities and NIR-II Responsiveness to Fight against Deep Tissue Infections

Hydrophobic and flame-retardant coating on silk via ferric salt chelation

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