Cu/Zn superoxide dismutase and ascorbate peroxidase enhance in vitro shoot multiplication in transgenic plum

Faize, Mohamed; Faize, Lydia; Petri, César; Barba‐Espín, Gregorio; Díaz‐Vivancos, Pedro; Clemente‐Moreno, María José; Koussa, Tayeb; Rifai, Lalla Aïcha; Burgos, L.; Hernández, José Antonio

doi:10.1016/j.jplph.2012.12.016

Cited by 34 publications

(20 citation statements)

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“…Similarly, the activity of antioxidant enzymes increased during organogenesis in Brassica rapa var. turnip [33], Caladium bicolor [34], Crocus sativus [35], Piper nigrum [9], and plum [36]. The activities of SOD and POD increased significantly with increasing concentration of Si in the shoot induction medium.…”

Section: Resultsmentioning

confidence: 99%

Silicon Promotes Adventitious Shoot Regeneration and Enhances Salinity Tolerance ofAjuga multifloraBunge by Altering Activity of Antioxidant Enzyme

Sivanesan

Jeong

2014

The Scientific World Journal

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We investigated the effect of Si concentration on shoot regeneration and salinity tolerance of Ajuga multiflora. Addition of Si to the shoot induction medium significantly increased the frequency of shoot induction. The average number of shoots regenerated per explant decreased on the medium containing NaCl alone, while there was less decrease when the shoot induction medium was supplemented with both NaCl and Si. The shoot induction percentage increased linearly with increasing concentration of Si in the NaCl containing medium. Addition of Si to the shoot induction medium significantly increased SOD, POD, APX, and CAT activity in regenerated shoot buds as compared with the control. The inclusion of Si to the NaCl containing medium significantly increased the SOD activity in leaves and roots, while it decreased POD, APX, and CAT activity in both organs. Scanning electron microscopic analysis showed that there are no distinct differences in the structure of stomata between the control and Si-treated plants. However, NaCl treatment significantly affected the structure and number of stomata as compared to the control. Wavelength dispersive X-ray analysis confirmed the high Si deposition in trichomes of plants grown in the Si containing medium but not in plants grown in the medium without Si.

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Section: Resultsmentioning

confidence: 99%

Silicon Promotes Adventitious Shoot Regeneration and Enhances Salinity Tolerance ofAjuga multifloraBunge by Altering Activity of Antioxidant Enzyme

Sivanesan

Jeong

2014

The Scientific World Journal

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“…In that case, NaCl treatment (70 mM) decreased ASC contents up to 30% in the salt-tolerant cultivar and up to 50% in the salt-sensitive cultivar (Hern andez et al, 2000). In spite of their presumed role as damaging agents, ROS acts as signalling molecules in regulation of stress responses as well as in developmental regulation (Barba-Espin et al, 2010, 2011bFaize et al, 2013). It is well known that crosstalk between Ca 2+ and ROS modulates the activity of specific proteins involved, directly or indirectly, in plant defence responses.…”

Section: Discussionmentioning

confidence: 98%

Ectopic expression of cytosolic superoxide dismutase and ascorbate peroxidase leads to salt stress tolerance in transgenic plums

Díaz‐Vivancos

Faize

Barba‐Espín

et al. 2013

Plant Biotechnology Journal

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132

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These authors have contributed equally to this work.Keywords: antioxidative metabolism, cytosolic ascorbate peroxidase, cytosolic superoxide dismutase, genetic engineering, Prunus domestica, salt stress. SummaryTo fortify the antioxidant capacity of plum plants, genes encoding cytosolic antioxidants ascorbate peroxidase (cytapx) and Cu/Zn-superoxide dismutase (cytsod) were genetically engineered in these plants. Transgenic plum plants expressing the cytsod and/or cytapx genes in cytosol have been generated under the control of the CaMV35S promoter. High levels of cytsod and cytapx gene transcripts suggested that the transgenes were constitutively and functionally expressed. We examined the potential functions of cytSOD and cytAPX in in vitro plum plants against salt stress (100 mM NaCl). Several transgenic plantlets expressing cytsod and/or cytapx showed an enhanced tolerance to salt stress, mainly lines C5-5 and J8-1 (expressing several copies of sod and apx, respectively). Transformation as well as NaCl treatments influenced the antioxidative metabolism of plum plantlets, including enzymatic and nonenzymatic antioxidants. Transgenic plantlets exhibited higher contents of nonenzymatic antioxidants glutathione and ascorbate than nontransformed control, which correlated with lower accumulation of hydrogen peroxide. Overall, our results suggest that transformation of plum plants with genes encoding antioxidant enzymes enhances the tolerance to salinity.

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“…PPO activity was measured as described by Masia, Ventura, Gemma, and Sansavini (1998) and expressed as ΔOD per minute per milligram of protein. CAT and APX were determined as described by Faize et al (2013).…”

Section: Enzymes Extractions Activities and H 2 O 2 Determinationmentioning

confidence: 99%

Priming of Nicotiana benthamiana antioxidant defences using brown seaweed extracts

Esserti

Smaili

Makroum

et al. 2017

Journal of Phytopathology

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Extracts from the brown seaweeds Cystoseira myriophylloides, Laminaria digitata and Fucus spiralis were evaluated as plant defence inducers against the wild fire disease of Nicotiana benthamiana. Seeds’ imbibition in aqueous seaweed extracts (SE) results in plants with reduced disease severity. In addition, bacterial populations were significantly reduced in these plants when compared to those germinated in sterile distilled water. They primed N. benthamiana for H2O2 accumulation and for enhanced activity of several antioxidant enzymes including catalase, ascorbate peroxidase, guaiacol peroxidase and polyphenol oxidase. These results revealed that soaking of seeds in SE before sowing allows N. benthamiana to reduce pathogen attack and can be easily applied in practice.

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Cu/Zn superoxide dismutase and ascorbate peroxidase enhance in vitro shoot multiplication in transgenic plum

Cited by 34 publications

References 34 publications

Silicon Promotes Adventitious Shoot Regeneration and Enhances Salinity Tolerance ofAjuga multifloraBunge by Altering Activity of Antioxidant Enzyme

Silicon Promotes Adventitious Shoot Regeneration and Enhances Salinity Tolerance ofAjuga multifloraBunge by Altering Activity of Antioxidant Enzyme

Ectopic expression of cytosolic superoxide dismutase and ascorbate peroxidase leads to salt stress tolerance in transgenic plums

Priming of Nicotiana benthamiana antioxidant defences using brown seaweed extracts

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