Ectopic expression of cytosolic superoxide dismutase and ascorbate peroxidase leads to salt stress tolerance in transgenic plums

Díaz‐Vivancos, Pedro; Faize, Mohamed; Barba‐Espín, Gregorio; Faize, Lydia; Petri, César; Hernández, José Antonio; Burgos, L.

doi:10.1111/pbi.12090

Cited by 132 publications

(82 citation statements)

References 68 publications

(121 reference statements)

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“…These results agreed with those of previous studies (Badawi et al, 2004;Diaz-Vivancos et al, 2013). Saline induced an increase in the ROS level, leading to seed deterioration (Xi et al, 2010).…”

Section: Discussionsupporting

confidence: 93%

A Cu/Zn superoxide dismutase from Jatropha curcas enhances salt tolerance of Arabidopsis thaliana

et al. 2015

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ABSTRACT. Superoxide dismutases (SODs) are involved in protecting plants against diverse biotic and abiotic stresses. In the present study, a novel Cu/Zn-SOD gene (JcCu/Zn-SOD) was cloned from Jatropha curcas L. Quantitative reverse transcription-polymerase chain reaction analysis revealed that JcCu/Zn-SOD is constitutively expressed in different tissues of J. curcas and induced under NaCl treatment. To characterize the function of this gene with respect to salt tolerance, the construct p35S:JcCu/Zn-SOD was developed and transformed into Arabidopsis using Agrobacterium-mediated transformation. Compared with wild-type, transgenic plants over-expressing JcCu/Zn-SOD showed enhanced tolerance to salt stress during germination, seedling establishment, and growth in terms of longer root, larger rosette area, and a larger number of leaves in addition to higher SOD activity levels 2087 SOD from Jatropha curcas enhances salt tolerance ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (1): 2086-2098(2015 under NaCl stress. In addition, over-expression of JcCu/Zn-SOD resulted in lower monodialdehyde content in transgenic Arabidopsis compared to wild-type plants under the same NaCl stress. Therefore, JcCu/Zn-SOD can increase a plant salt stress tolerance potentially by reducing oxidant injury.

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Section: Discussionsupporting

confidence: 93%

A Cu/Zn superoxide dismutase from Jatropha curcas enhances salt tolerance of Arabidopsis thaliana

et al. 2015

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“…In this study, all transgenic plum lines had lower levels of H2O2 than non-transformed wild-type plants under salt exposure (100 mM) [211].…”

Section: Ros and Rns Generationmentioning

confidence: 55%

Plant Responses to Salt Stress: Adaptive Mechanisms

Acosta‐Motos¹,

Ortuño²,

Bernal‐Vicente³

et al. 2017

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This review deals with the adaptive mechanisms that plants can implement to cope with the challenge of salt stress. Plants tolerant to NaCl implement a series of adaptations to acclimate to salinity, including morphological, physiological and biochemical changes. These changes include increases in the root/canopy ratio and in the chlorophyll content in addition to changes in the leaf anatomy that ultimately lead to preventing leaf ion toxicity, thus maintaining the water status in order to limit water loss and protect the photosynthesis process. Furthermore, we deal with the effect of salt stress on photosynthesis and chlorophyll fluorescence and some of the mechanisms thought to protect the photosynthetic machinery, including the xanthophyll cycle, photorespiration pathway, and water-water cycle. Finally, we also provide an updated discussion on salt-induced oxidative stress at the subcellular level and its effect on the antioxidant machinery in both salt-tolerant and salt-sensitive plants. The aim is to extend our understanding of how salinity may affect the physiological characteristics of plants.

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“…Although in planta overexpression of SOD genes often increase endogenous H 2 O 2 levels, such plants are less sensitive to stress-related necrosis if sufficient H 2 O 2 -degradation is conferred by elevated expression of antioxidants like e.g. catalases and peroxidases (Kwon et al, 2002;Tang et al, 2006;Lee et al, 2007;Faize et al, 2011Faize et al, , 2012Diaz-Vivancos et al, 2013). We found that S1 tobacco indeed displays elevated expression of an endogenous glutathione peroxidase (NtGPX) and a tau class glutathione S-transferase (NtGSTU1b), genes that may take part in the degradation of H 2 O 2 and lipid hydroperoxides during oxidative stress.…”

Section: Discussionmentioning

confidence: 99%

“…Indeed, transgenic SOD-overexpressing tobacco that is tolerant to necrosis caused by high light intensity and low temperature, also displays elevated levels of endogenous APX (Sen Gupta et al, 1993). Transgenic plants that simultaneously overexpress a SOD and an APX gene show enhanced tolerance to necrosis caused by various abiotic stresses and pathogenic infections (Kwon et al, 2002;Tang et al, 2006;Lee et al, 2007;Faize et al, 2011Faize et al, , 2012Diaz-Vivancos et al, 2013).…”

mentioning

confidence: 99%

Catalases may play different roles in influencing resistance to virus-induced hypersensitive necrosis

Viczián¹,

Künstler²,

Hafez³

et al. 2014

Acta Phytopathologica et Entomologica Hungarica

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High concentrations of the reactive oxygen species (ROS) superoxide (O 2•-) and hydrogen peroxide (H 2 O 2 ) contribute to the induction of plant cell and tissue death (necrosis). In an effort to create transgenic plants with high antioxidant capacity that could resist necrotic symptoms we produced two transgenic tobacco (Nicotiana tabacum cv. SR1) lines (S1 and S2) overexpressing a tomato chloroplast superoxide dismutase (SlChSOD). SOD genes encode for antioxidant enzymes that dismutate superoxide to hydrogen peroxide. Therefore, SOD-overproducing plants may contain high levels of hydrogen peroxide and are sensitive to stress-related necrosis unless sufficient degradation of hydrogen peroxide is conferred by elevated expression of antioxidants like e.g. catalases and peroxidases. Indeed, line S1 displayed elevated expression of a glutathione peroxidase (NtGPX) and a glutathione S-transferase (NtGSTU1b), as compared to wild type plants. Interestingly, however, expression of a catalase (NtCAT1) was repressed in both SOD-overexpressing lines. This predicts that such plants could be sensitive to localized necrosis (HR) caused by virus infection, since repression of NtCAT1 has been shown to occur during virus-induced HR (e.g. Dorey et al., 1998;Künstler et al., 2007). To elucidate whether other catalases might play a role in resistance to virus induced HR-type necrotic symptoms, a maize catalase (ZmCat2) was transiently overexpressed in Nicotiana edwardsonii and N. edwardsonii var. Columbia plants by agroinfiltration. Inoculation of agroinfiltrated plants with Tobacco mosaic virus (TMV) revealed that ZmCat2 confers enhanced resistance to HR-type necrosis during TMV infection. It seems that catalases may play different roles in influencing resistance to virus-induced hypersensitive necrosis.

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Ectopic expression of cytosolic superoxide dismutase and ascorbate peroxidase leads to salt stress tolerance in transgenic plums

Cited by 132 publications

References 68 publications

A Cu/Zn superoxide dismutase from Jatropha curcas enhances salt tolerance of Arabidopsis thaliana

A Cu/Zn superoxide dismutase from Jatropha curcas enhances salt tolerance of Arabidopsis thaliana

Plant Responses to Salt Stress: Adaptive Mechanisms

Catalases may play different roles in influencing resistance to virus-induced hypersensitive necrosis

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