Cucumis

Chen, Jinfeng; Zhou, Xiaohui

doi:10.1007/978-3-642-20450-0_6

Cited by 8 publications

(8 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Melon ( Cucumis melo L.) belongs to the Cucurbitaceae family and is a diploid species having 2 n = 2 x = 24 chromosomes [ 1 ]. Detailed karyotype analysis in the Cucumis genus, particularly in melon, has been difficult to achieve because of their small chromosome sizes and poor stainability [ 2 , 3 ]. In addition, the identification of secondary constrictions and the procurement of more detailed chromatin images are also difficult, even when using properly dispersed metaphase chromosomes, because of their highly condensed status.…”

Section: Introductionmentioning

confidence: 99%

An improved method for inducing prometaphase chromosomes in plants

et al. 2018

View full text Add to dashboard Cite

BackgroundDetailed karyotyping using metaphase chromosomes in melon (Cucumis melo L.) remains a challenge because of their small chromosome sizes and poor stainability. Prometaphase chromosomes, which are two times longer and loosely condensed, provide a significantly better resolution for fluorescence in situ hybridization (FISH) than metaphase chromosomes. However, suitable method for acquiring prometaphase chromosomes in melon have been poorly investigated.ResultsIn this study, a modified Carnoy’s solution II (MC II) [6:3:1 (v/v) ethanol: acetic acid: chloroform] was used as a pretreatment solution to obtain prometaphase chromosomes. We demonstrated that the prometaphase chromosomes obtained using the MC II method are excellent for karyotyping and FISH analysis. We also observed that a combination of MC II and the modified air dry (ADI) method provides a satisfactory meiotic pachytene chromosome preparation with reduced cytoplasmic background and clear chromatin spreads. Moreover, we demonstrated that pachytene and prometaphase chromosomes of melon and Abelia × grandiflora generate significantly better FISH images when prepared using the method described. We confirmed, for the first time, that Abelia × grandiflora has pairs of both strong and weak 45S ribosomal DNA signals on the short arms of their metaphase chromosomes.ConclusionThe MC II and ADI method are simple and effective for acquiring prometaphase and pachytene chromosomes with reduced cytoplasm background in plants. Our methods provide high-resolution FISH images that can help accelerate molecular cytogenetic research in plants.

show abstract

Section: Introductionmentioning

confidence: 99%

An improved method for inducing prometaphase chromosomes in plants

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Karyotype analysis in Cucumis is difficult due to the small size and poor stainability of chromosomes [ 20 ]. Despite this, karyotypes based on chromosome banding and morphology have been developed for several melon types with varying results [ 21 - 23 ].…”

Section: Introductionmentioning

confidence: 99%

Use of targeted SNP selection for an improved anchoring of the melon (Cucumis melo L.) scaffold genome assembly

et al. 2015

View full text Add to dashboard Cite

BackgroundThe genome of the melon (Cucumis melo L.) double-haploid line DHL92 was recently sequenced, with 87.5 and 80.8% of the scaffold assembly anchored and oriented to the 12 linkage groups, respectively. However, insufficient marker coverage and a lack of recombination left several large, gene rich scaffolds unanchored, and some anchored scaffolds unoriented. To improve the anchoring and orientation of the melon genome assembly, we used resequencing data between the parental lines of DHL92 to develop a new set of SNP markers from unanchored scaffolds.ResultsA high-resolution genetic map composed of 580 SNPs was used to anchor 354.8 Mb of sequence, contained in 141 scaffolds (average size 2.5 Mb) and corresponding to 98.2% of the scaffold assembly, to the 12 melon chromosomes. Over 325.4 Mb (90%) of the assembly was oriented. The genetic map revealed regions of segregation distortion favoring SC alleles as well as recombination suppression regions coinciding with putative centromere, 45S, and 5S rDNA sites. New chromosome-scale pseudomolecules were created by incorporating to the previous v3.5 version an additional 38.3 Mb of anchored sequence representing 1,837 predicted genes contained in 55 scaffolds. Using fluorescent in situ hybridization (FISH) with BACs that produced chromosome-specific signals, melon chromosomes that correspond to the twelve linkage groups were identified, and a standardized karyotype of melon inbred line T111 was developed.ConclusionsBy utilizing resequencing data and targeted SNP selection combined with a large F2 mapping population, we significantly improved the quantity of anchored and oriented melon scaffold genome assembly. Using genome information combined with FISH mapping provided the first cytogenetic map of an inodorus melon type. With these results it was possible to make inferences on melon chromosome structure by relating zones of recombination suppression to centromeres and 45S and 5S heterochromatic regions. This study represents the first steps towards the integration of the high-resolution genetic and cytogenetic maps with the genomic sequence in melon that will provide more information on genome organization and allow for the improvement of the melon genome draft sequence.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-014-1196-3) contains supplementary material, which is available to authorized users.

show abstract

“…This region might have originated from a related wild species. Indeed, wide-cross attempts between cultivated and wild Cucumis species, such as C. melo × C. metuliferus and C. metuliferus x C. dipsaceus , resulted in viable offspring ( Van Raamsdonk et al 1989 ; Chen and Zhou 2011 ). Also C. melo × C. dipsaceus resulted in fruit induction, although the fruits did not contain seeds.…”

Section: Resultsmentioning

confidence: 99%

Chasing breeding footprints through structural variations inCucumis meloand wild relatives

Demırcı

Fuentes

Dooijeweert

et al. 2020

G3 Genes|Genomes|Genetics

View full text Add to dashboard Cite

Cucumis melo (melon or muskmelon) is an important crop in the family of the Cucurbitaceae. Melon is cross pollinated and domesticated at several locations throughout the breeding history, resulting in highly diverse genetic structure in the germplasm. Yet, the relations among the groups and cultivars are still incomplete. We shed light on the melonbreeding history, analyzing structural variations ranging from 50 bp up to 100 kb, identified from whole genome sequences of 100 selected melon accessions and wild relatives. Phylogenetic trees based on SV types completely resolve cultivars and wild accessions into two monophyletic groups and clustering of cultivars largely correlates with their geographic origin. Taking into account morphology, we found six mis-categorized cultivars. Unique inversions are more often shared between cultivars, carrying advantageous genes and do not directly originate from wild species. Approximately 60% of the inversion breaks carry a long poly A/T motif, and following observations in other plant species, suggest that inversions in melon likely resulted from meiotic recombination events. We show that resistance genes in the linkage V region are expanded in the cultivar genomes compared to wild relatives. Furthermore, particular agronomic traits such as fruit ripening, fragrance, and stress response are specifically selected for in the melon subspecies. These results represent distinctive footprints of selective breeding that shaped today’s melon. The sequences and genomic relations between land races, wild relatives, and cultivars will serve the community to identify genetic diversity, optimize experimental designs, and enhance crop development.

show abstract

Cucumis

Cited by 8 publications

References 53 publications

An improved method for inducing prometaphase chromosomes in plants

An improved method for inducing prometaphase chromosomes in plants

Use of targeted SNP selection for an improved anchoring of the melon (Cucumis melo L.) scaffold genome assembly

Chasing breeding footprints through structural variations inCucumis meloand wild relatives

Contact Info

Product

Resources

About