Cucurbit biotechnology-the importance of virus resistance

Gaba, Victor; Zelcer, Aaron; Gal‐On, Amit

doi:10.1079/ivp2004554

Cited by 91 publications

(42 citation statements)

References 74 publications

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“…In general, the factors influencing transformation efficiency include plant species, explants types, maturity, researcher skill, selectable marker, etc. (Gaba et al 2004). In this study, the difference of transformation frequency from other studies may be speculated that the maturity of immature embryos was affected.…”

Section: Generation Of Transgenic Maize Plantsmentioning

confidence: 49%

Expression of Dengue virus EIII domain-coding gene in maize as an edible vaccine candidate

Kim¹,

Kwon²,

Yang³

et al. 2014

Journal of Plant Biotechnology

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Plant-based vaccines possess some advantages over other types of vaccine biotechnology such as safety, low cost of mass vaccination programs, and wider use of vaccines for medicine. This study was undertaken to develop the transgenic maize as edible vaccine candidates for humans. The immature embryos of HiII genotype were inoculated with A. tumefaciens strain C58C1 containing the binary vectors (V662 or V663). The vectors carrying nptII gene as selection marker and scEDIII (V662) or wCTB-scEDIII (V663) target gene, which code EIII proteins inhibite viral adsorption by cells. In total, 721 maize immature embryos were transformed and twenty-two putative transgenic plants were regenerated after 12 weeks selection regime. Of them, two-and six-plants were proved to be integrated with scEDIII and wCTB-scEDIII genes, respectively, by Southern blot analysis. However, only one plant (V662-29-3864) can express the gene of interest confirmed by Northern blot analysis. These results demonstrated that this plant could be used as a candidated source of the vaccine production.

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Section: Generation Of Transgenic Maize Plantsmentioning

confidence: 49%

Expression of Dengue virus EIII domain-coding gene in maize as an edible vaccine candidate

Kim¹,

Kwon²,

Yang³

et al. 2014

Journal of Plant Biotechnology

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“…Conventional breeding aiming to transfer desirable traits from wild species has not been successful (Esquinas-Alcazer & Gulick, 1983). Therefore, the development of in vitro micropropagation methods is very useful for its clonal multiplication (Deakin et al, 1971;Gaba et al, 2004). Tissue culture methods allow establishing cultures from a minimum amount of starting plant material (Klavina et al, 2006), with a lower chance of obtaining mutants comparing with conventional breeding.…”

Section: Introductionmentioning

confidence: 99%

Kinetin Is the Most Effective Cytokinin on Shoot Multiplication from Cucumber

Abu-Romman¹,

Al-Hadid²,

Arabiyyat³

2015

JAS

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In vitro shoot multiplication of cucumber (Cucumis sativus) was examined from the nodal explants of 10-day-old aseptic plantlets using Murashige and Skoog (MS) media supplemented with different concentration (0, 0.5, 1, 2, 3 mg L -1 ) of cytokinins (6-Benzylaminopurine-BAP, Kinetin-Kn, Thidiazuron-TDZ, and Zeatin). Nodal explants of cucumber showed shoot induction and multiplication in response to all cytokinins tried. MS medium containing Kn was the most effective for inducing shoots from nodal explants of cucumber. The maximum rate of regeneration (83%), the highest number of obtained shoots (7.93 shoots/explant) and the longest shoots (3.61 cm) were obtained on MS medium fortified with 1 mg L -1 Kn. The lowest culture responses were recorded for media supplemented with either BAP or Zeatin. In conclusion, using Kn is strongly recommended than using Zeatin, TDZ and BAP to obtain the highest percentage of regeneration, the highest number of shoot/explant, and the highest shoot length for nodal explants of cucumber.

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“…Direct control of CMV or prevention of its transmission by aphids by using insecticides is difficult to achieve. One promising approach for controlling CMV is the use of pathogen-derived transgenes in genetically modified plants (Palukaitis & Zaitlin, 1997;Gaba et al, 2004). Pathogenderived resistance to CMV and other viruses works either through the triggering of RNA silencing against the transgene-encoded RNA, or through disruption of one or more stages of viral infection by constitutive expression of wild-type or mutant viral proteins by the host plant (Hellwald & Palukaitis, 1995;Beachy, 1997;Wintermanteldescribed as early as the 1920s by McKinney (1929) who showed that tobacco plants that had previously been inoculated with a tobacco mosaic virus (TMV) strain causing mild green mosaic symptoms were resistant to a subsequent challenge with a TMV strain that caused yellow mosaic symptoms.…”

Section: Introductionmentioning

confidence: 99%

A cucumber mosaic virus mutant lacking the 2b counter-defence protein gene provides protection against wild-type strains

Ziebell

Payne

Berry

et al. 2007

Journal of General Virology

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Several plant virus mutants, in which genes encoding silencing suppressor proteins have been deleted, are known to induce systemic or localized RNA silencing against themselves and other RNA molecules containing homologous sequences. Thus, it is thought that many cases of cross-protection, in which infection with a mild or asymptomatic virus mutant protects plants against challenge infection with closely related virulent viruses, can be explained by RNA silencing. We found that a cucumber mosaic virus (CMV) mutant of the subgroup IA strain Fny (Fny-CMVD2b), which cannot express the 2b silencing suppressor protein, cross-protects tobacco (Nicotiana tabacum) and Nicotiana benthamiana plants against disease induction by wild-type Fny-CMV. However, protection is most effective only if inoculation with Fny-CMVD2b and challenge inoculation with wild-type CMV occurs on the same leaf. Unexpectedly, FnyCMVD2b also protected plants against infection with TC-CMV, a subgroup II strain that is not closely related to Fny-CMV. Additionally, in situ hybridization revealed that Fny-CMVD2b and Fny-CMV can co-exist in the same tissues but these tissues contain zones of Fny-CMVD2b-infected host cells from which Fny-CMV appears to be excluded. Taken together, it appears unlikely that cross-protection by Fny-CMVD2b occurs by induction of systemic RNA silencing against itself and homologous RNA sequences in wild-type CMV. It is more likely that protection occurs through either induction of very highly localized RNA silencing, or by competition between strains for host cells or resources. INTRODUCTIONCucumber mosaic virus (CMV) is the type species of the genus Cucumovirus of the family Bromoviridae (Van Regenmortel et al., 2000). Based on serological relationships and sequence criteria, the species is divided into three distinct subgroups: IA, IB and II (Roossinck et al., 1999). CMV has the largest host range of any known plant virus and is transmitted in a non-persistent manner by aphids belonging to over 80 species. Taken together, these factors probably contribute to the worldwide distribution of the virus and its economic importance (Palukaitis et al., 1992;Palukaitis & García-Arenal, 2003). The CMV genome consists of three positive-sense RNA molecules. These are RNAs 1, 2 and 3, which also function as mRNAs for the synthesis of the 1a and 2a replicase proteins, and the 3a movement protein, respectively. During replication, subgenomic mRNAs encoding additional proteins are synthesized. The subgenomic RNA 4, derived from RNA 3, is the mRNA for CMV coat protein (CP) and RNA 4A, which is derived from RNA 2, is the mRNA for the multifunctional 2b protein (Ding et al., 1994). Direct control of CMV or prevention of its transmission by aphids by using insecticides is difficult to achieve. One promising approach for controlling CMV is the use of pathogen-derived transgenes in genetically modified plants (Palukaitis & Zaitlin, 1997;Gaba et al., 2004). Pathogenderived resistance to CMV and other viruses works either through the triggering of...

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Cucurbit biotechnology-the importance of virus resistance

Cited by 91 publications

References 74 publications

Expression of Dengue virus EIII domain-coding gene in maize as an edible vaccine candidate

Expression of Dengue virus EIII domain-coding gene in maize as an edible vaccine candidate

Kinetin Is the Most Effective Cytokinin on Shoot Multiplication from Cucumber

A cucumber mosaic virus mutant lacking the 2b counter-defence protein gene provides protection against wild-type strains

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