Kinetin Is the Most Effective Cytokinin on Shoot Multiplication from Cucumber

Abu-Romman, Saeid; Al-Hadid, Khaldoun J.; Arabiyyat, Abdullah R.

doi:10.5539/jas.v7n10p159

Cited by 30 publications

(20 citation statements)

References 35 publications

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“…For example, the multiplication of a medicinal plant (Scopari dulcis) showed that shoot induction was improved by a combination of both BAP and KIN (Premkumar et al, 2011). On the contrary, for cucumbers, the best results of the shoot multiplication stage were obtained when only kinetin was supplemented to the MS medium (Abu-Romman et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Shoot multiplication of two <em>Sequoia sempervirens </em> genotypes with addition of small concentrations of kinetin

et al. 2019

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Sequoia sempervirens (D. Don) Endl. é uma conífera que produz madeira de elevada qualidade, com potencial de aplicação na indústria. No entanto, a espécie exibe baixas taxas de germinação. Este estudo foi dedicado à otimizar um protocolo para a multiplicação de brotos de S. sempervirens, através da micropropagação. Dois genótipos, adaptados ao Sul do Brasil, foram a fonte de explantes para o estudo da multiplicação in vitro. Utilizou-se o meio de cultura MS (Murashige Skoog) (50% de sua concentração original) complementado com reguladores de crescimento sob duas abordagens. Para a multiplicação, foram combinados ácido α-naftaleno acético (ANA) e 6-benzilaminopurina (BAP), em concentrações distintas. No segundo experimento foram testadas, separadamente, três concentrações de cintinas (BAP, cinetina e 2-isopentenladenina). ANA , na concentração 0,1 mg L-1, permitiu a produção de brotos com maior comprimento médio do ramo principal. O uso de cinetina, em baixas concentrações, proporcionou o melhor desempenho para a multiplicação de brotos, mas com desempenhos distintos, de acordo com o genótipo. Descrevemos uma recomendação dos reguladores de crescimento de plantas mais adequados e suas concentrações para a multiplicação de brotos de S. sempervirens, o que pode auxiliar trabalhos voltados para enraizamento e aclimatização adventícia.

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Section: Discussionmentioning

confidence: 99%

Shoot multiplication of two <em>Sequoia sempervirens </em> genotypes with addition of small concentrations of kinetin

et al. 2019

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“…Cytokinins are commonly used in plant tissue culture to initiate cell growth and also to induce the formation of shoots whereby the are actively take part in various plant physiological processes ( Davies 1995 ). This includes shoot multiplication ( Kaviani et al 2013 ; Bhat et al 2013 ; Abu-Romman et al 2015 ), tuberous root production ( Deshwal & Trivedi 2011 ), callus induction ( Borjian & Arak 2013 ), leaf senescence ( Riefler et al 2006 ) and shoot apical meristem activity regulators ( Tucker & Laux 2007 ). Cytokinin such as kinetin is known to initiate cell division in the presence of auxin and is widely used together with auxins for callus formation or to induce shoots where low levels of auxin is available.…”

Section: Introductionmentioning

confidence: 99%

Shoot Induction in White Eggplant (Solanum melongena L. Cv. Bulat Putih) using 6-Benzylaminopurine and Kinetin

Foo

Lee

Chin

et al. 2018

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Solanum melongena L. commonly known as the eggplant or brinjal comes from the family of Solanaceae, sharing the same ancestor with the tomato and potato. It is an economically important crop worldwide, being well studied for its medicinal properties, nutritional values and its role as an alternative model plant. The eggplant fruit has been previously used for treatments of various diseases such as bronchitis, asthma, arthritis and diabetes as well as its nutritive properties that are beneficial to the human diet. Plant transformation studies on the eggplant have been widely done for the production of transgenic eggplants harbouring genes that are beneficial for optimal plant growth and fruit production. Shoot induction is an essential step required for the successful regeneration of transformed plant tissues and therefore is an essential pre-requisite in Agrobacterium-mediated transformation. The local eggplant cv. Bulat Putih is a local cultivar of eggplant in Malaysia with white and round fruits making it a potential model plant colour pigment accumulation studies in fruit crops. The current work aims to investigate the shoot induction potential of 6-benzylaminopurine (BAP) and Kinetin from cotyledon explants of eggplant cv. Bulat Putih. Results indicated that both BAP and Kinetin were able to induce the regeneration of callus from cotyledon explants. On the other hand, Kinetin at the concentration of 2.0 mg/L successfully induced shoots at the value of 1.50 ± 0.22 shoots per explant, whereas BAP alone did not trigger any formation of shoots. This study indicated that kinetin alone is sufficient to induce shoots in eggplant cv. Bulat Putih without the presence of BAP.

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“…However, HgCl 2 solution is highly toxic and it is therefore recommended for frequent use in sterilization procedure. Growth regulators especially cytokinins are one of the most important factors affecting the regeneration of microshoots [30][31][32][33]. Kinetin, BAP, TDZ and Zeatin have been used in cassava micropropagation [17,[34][35][36].…”

Section: Discussionmentioning

confidence: 99%

“…Growth regulators especially cytokinins are one of the most important factors affecting the regeneration of microshoots [ 30 – 33 ]. Kinetin, BAP, TDZ and Zeatin have been used in cassava micropropagation [ 17 , 34 – 36 ].…”

Section: Discussionmentioning

confidence: 99%

In vitro propagation of three mosaic disease resistant cassava cultivars

et al. 2020

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Background Cassava is a staple food for over 800 million people globally providing a cheap source of carbohydrate. However, the cultivation of cassava in the country is facing to viral diseases, particularly cassava mosaic disease (CMD) which can cause up to 95% yield losses. With aim to supply farmers demand for clean planting materials, there is need to accelerate the production of the elite cultivars by use of tissue culture in order to cope with the demand. Methods Nodal explants harvested from the greenhouse grown plants were sterilised using different concentrations of a commercial bleach JIK (3.85% NaOCl) and varying time intervals. Microshoots induction was evaluated using thidiazuron (TDZ), benzyl amino purine (BAP), and kinetin. Rooting was evaluated using different auxins (Naphthalene acetic acid NAA and Indole-3-butyricacid IBA). PCR-based SSR and SCAR markers were used to verify the presence of CMD2 gene in the regenerated plantlets. Results The highest level of sterility in explants (90%) was obtained when 20% Jik was used for 15 min. The best cytokinin for microshoots regeneration was found to be kinetin with optimum concentrations of 5, 10 and 20 μM for Agric-rouge, Atinwewe, and Agblehoundo respectively. Medium without growth regulators was the best for rooting the three cultivars. A survival rate of 100, 98, and 98% was recorded in the greenhouse for Agric-rouge, Atinwewe, and Agblehoundo respectively and the plantlets appeared to be morphologically normal. The SSR and SCAR analysis of micropropagated plants showed a profile similar to that of the mother plants indicating that the regenerated plantlets retained the CMD2 gene after passing through in vitro culture, as expected with micropropagation. Conclusion The nodal explants was established to be 20% of Jik (3.85% NaOCl) with an exposure time of 15 min. Kinetin was proved to be the best cytokinins for microshoot formation with the optimum concentration of 5, 10 and 20 μM for Agric-rouge, Atinwewe, and Agblehoundo respectively. The protocol developed during this study will be useful for mass propagation of the elite cassava cultivars.

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Kinetin Is the Most Effective Cytokinin on Shoot Multiplication from Cucumber

Cited by 30 publications

References 35 publications

Shoot multiplication of two <em>Sequoia sempervirens </em> genotypes with addition of small concentrations of kinetin

Shoot multiplication of two <em>Sequoia sempervirens </em> genotypes with addition of small concentrations of kinetin

Shoot Induction in White Eggplant (Solanum melongena L. Cv. Bulat Putih) using 6-Benzylaminopurine and Kinetin

In vitro propagation of three mosaic disease resistant cassava cultivars

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