Cucurbits powdery mildew race identity and reaction of melon genotypes1

Rabelo, Hudson de Oliveira; Santos, Lucas da Silva; Diniz, Guilherme Matos Martins; Marín, Manuel; Braz, Leila Trevisan; McCreight, James D.

doi:10.1590/1983-40632017v4749537

Cited by 15 publications

(9 citation statements)

References 16 publications

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“…Previous studies demonstrated that disease resistance screening was important to get tolerant germplasm for breeding new cultivars to control powdery mildew in field and greenhousegrown pumpkins (Luitel et al, 2016;Rabelo et al, 2017). Furthermore, the activation speeds and activity levels of defense enzymes vary in different plant genotypes or plant-pathogen interactions (Yan et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

Differential Responses of Cucurbita pepo to Podosphaera xanthii Reveal the Mechanism of Powdery Mildew Disease Resistance in Pumpkin

Zhang

Liu

et al. 2021

Front. Plant Sci.

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Powdery mildew is one of the most destructive diseases and the major cause to the production losses of cucurbit worldwide. A number of strategies have been developed and applied to discover suitable and safer methods to manage the occurrence of powdery mildew disease in pumpkins (Cucurbita pepo L.), but information is limited in screening tolerant germplasms and exploring their mechanisms in preventing the disease occurrence at physiological, biochemical, and molecular levels. Therefore, we investigated the responses of two commercial pumpkin cultivars to Podosphaera xanthii infection. Compared with mock-inoculated seedlings, few small and sparse powdery areas were observed on the leaves of the Sixing F1 cultivar on the 13th day after inoculation with P. xanthii, whereas a large number of diseased powdery areas and a layer of white powdery mildew were observed on the surface of Jin12 F1 leaves. The inoculation duration (7, 9, 11, and 13 days) significantly and continuously increased the disease incidence and index of pumpkin seedlings. The contents of H2O2, MDA, lignin, and total phenolics in the leaves of Sixing F1 and Jin12 F1 cultivars were markedly increased after inoculation with P. xanthii. However, the Sixing F1 cultivar exhibited much less reactive oxygen species (ROS) accumulation, a lower rate of lipid peroxidation, and a higher level of lignin and total phenolics contents after inoculation than the Jin12 F1 cultivar. Compared with untreated control pumpkin seedlings, significantly higher activities and gene expressions of the phenylpropanoids pathway enzymes (PAL and PPO), ROS scavenging defense enzymes (SOD, CAT, POD, and APX), and other salicylic acid (SA) signaling pathway marker genes were observed in the leaves of both cultivars after P. xanthii inoculation at different inoculation time points. These enhancements were significantly higher in Sixing F1 than Jin12 F1. Our results indicate that the Sixing F1 cultivar exhibited a much stronger ability in resistance to P. xanthii infection than the Jin12 F1 cultivar. Our results suggest that one possible mechanism of C. pepo cultivars to prevent the pathogen P. xanthii infection is by activating and enhancing the activity and gene expression of the phenylpropanoids pathway to synthesize phenolic substances and lignin, ROS scavenging defense enzymes to eliminate the harmful effects of ROS, and signaling pathway marker gene expression to improve plant disease resistance.

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Section: Discussionmentioning

confidence: 99%

Differential Responses of Cucurbita pepo to Podosphaera xanthii Reveal the Mechanism of Powdery Mildew Disease Resistance in Pumpkin

Zhang

Liu

et al. 2021

Front. Plant Sci.

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“…P. xanthii is more frequently found in subtropical and tropical regions as well as in greenhouse crops (Natarajan et al 2016, Ning et al 2014. P. xanthii is emerging as the predominant pathogen in most countries (Bardin et al 1999, Hosoya et al 1999, Hudson et al 2018, McCreight 2006, Ning et al 2014, Pino et al 2002, Zhang et al 2012. Based on the reactions of isolates to melon differential lines, more than 28 physiological races of P. xanthii have been characterized (McCreight 2006).…”

Section: Resistance To Other Diseasesmentioning

confidence: 99%

Possibility of genome editing for melon breeding

Nonaka,

Ezura

2024

Breed. Sci.

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Genome editing technologies are promising for conventional mutagenesis breeding, which takes a long time to remove unnecessary mutations through backcrossing and create new lines because they directly modify the target genes of elite strains. In particular, this technology has advantages for traits caused by the loss of function. Many efforts have been made to utilize this technique to introduce valuable features into crops, including maize, soybeans, and tomatoes. Several genome-edited crops have already been commercialized in the US and Japan. Melons are an important vegetable crop worldwide, produced and used in various areas. Therefore, many breeding efforts have been made to improve its fruit quality, resistance to plant diseases, and stress tolerance. Quantitative trait loci (QTL) analysis was performed, and various genes related to important traits were identified. Recently, several studies have shown that the CRISPR/Cas9 system can be applied to melons, resulting in its possible utilization as a breeding technique. Focusing on two productivity-related traits, disease resistance, and fruit quality, this review introduces the progress in genetics, examples of melon breeding through genome editing, improvements required for breeding applications, and the possibilities of genome editing in melon breeding.

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“…Salah satu solusi efektif yang dapat dilakukan untuk mengontrol penyakit embun tepung adalah melalui pemuliaan tanaman dengan menggunakan kultivar tanaman yang resisten terhadap penyakit ini (Rabelo et al, 2017). Penelitian mengenai analisis ketahanan tanaman melon terhadap infeksi penyakit embun tepung sudah dilaporkan oleh beberapa peneliti (Aristya, Suryanto, Kasiamdari, & Daryono, 2013;C.…”

Section: Pendahuluanunclassified

Identification and Analysis of Powdery Mildew Resistance in Melon (Cucumis melo L.) Cultivar Meloni

Ishak

Daryono²

2020

BES

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Background: A powdery mildew-resistant cultivar of melon is needed to increase melon yield crops. Meloni is a superior melon cultivar bred through a crossing between ♀ SL-3 and ♂ PI 371795, resulted by the Laboratory of Genetics and Breeding, Faculty of Biology, UGM. This study aimed to determine the level resistance of Meloni to powdery mildew infection and to identify the powdery mildew species that infected Meloni based on morphological characters. Methods: Meloni seeds were germinated and planted in the greenhouse of PIAT UGM. Powdery mildew spores were inoculated into the leaves after ±2 weeks of age. Leaf infected were scored using the gridline every 3 days for 6 weeks. Scoring results were converted to the diseases index score. Furthermore, powdery mildew species was identified using morphological characters. Results: Meloni had a tolerance level of resistance to the powdery mildew infection. Based on the morphological characters with fibrosin bodies, conidia ovoid-shape and the position of the germ tube in the lateral part of the conidia, powdery mildew that infected Meloni was expected as P. xanthii. Conclusions: Meloni can be expected as an alternative to superior melon seeds resistant to pest and disease infections especially powdery mildew.

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Cucurbits powdery mildew race identity and reaction of melon genotypes1

Cited by 15 publications

References 16 publications

Differential Responses of Cucurbita pepo to Podosphaera xanthii Reveal the Mechanism of Powdery Mildew Disease Resistance in Pumpkin

Differential Responses of Cucurbita pepo to Podosphaera xanthii Reveal the Mechanism of Powdery Mildew Disease Resistance in Pumpkin

Possibility of genome editing for melon breeding

Identification and Analysis of Powdery Mildew Resistance in Melon (Cucumis melo L.) Cultivar Meloni

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