The present study was designed to analyze the different solvents effects on variation of different bioactive compounds and biological activities. The antioxidant activity of the thyme extracts was assessed by the determinations of total phenolic contents (TPC), total flavonoid contents (TFC), DPPH radical scavenging capacity and ferric reducing antioxidant power (FRAP); while qualitative and quantitative analysis of the individual phenolic acids was done by HPLC. Antimicrobial activity of extracts was evaluated by Disc diffusion method. The total extract yield from thyme using different solvents ranged from 9.6 -26.44 % (g/ 100g).Maximum extract yield was obtained with 100 % methanol while least with distill water. The values of TPC, TFC, DPPH radical scavenging activity (IC50) and FRAP (EC50) for thyme extracts ranged from 148.05 to 232.02 GAE mg/g of dry weight (DW), 25.17 to 162.20 CE mg/g DW, 0.97 to 4.15 mg/mL and 1248.05 to 2629.74 mg/mL, respectively. As per HPLC analysis, gallic acid and ρ-Coumaric acid with contribution 11.21 -98.76 mg/kg and 70.60 -578.59 mg/kg of extract, respectively, were detected to be the main phenolic acids in different extracts among others. Total amount of individual phenolic acids in different extracts varied over 36.05 to 719.77 mg/kg of extract. Highest amount of total phenolic acids was found with 80% methanol extract while the least with 100% methanol. Strongest antibacterial activity was shown by 80% ethanol extract. As far as extraction efficacy is concerned, methanol and ethanol were found to be most effective solvents among others towards extraction of antioxidants and phenolics from thyme.