Chemometric Profiling and Bioactivity of Verbena (Aloysia citrodora) Methanolic Extract from Four Localities in Tunisia

Tammar, Sonia; Salem, Nidhal; Wannes, Wissem Aidi; Limam, Hajer; Bourgou, Soumaya; Fares, Nedia; Dakhlaoui, Sarra; Hammami, Majdi; Khammassi, Saber; Re, G. Del; Hessini, Kamel; Msaâda, Kamel

doi:10.3390/foods10122912

Cited by 13 publications

(12 citation statements)

References 53 publications

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“…Our initial chemical analysis of HEAc revealed representative amounts of flavonoids consistent with the findings of Tammar et al [56] who determined the presence of these compounds in the leaves of A. citriodora collected from different locations. Similarly, Skaltsa and Shammas [57] isolated several flavones from the plant extract, and, subsequently, Carnat et al [58], Ragone et al [59], and Quirantes-Piné et al [52] detected the presence of flavone glucosides such as vitexin and isovitexin, in addition to apigenin-7-diglucuronide and chrysoeriol-7-diglucuronide.…”

Section: Discussionsupporting

confidence: 89%

Involvement of Anti-Inflammatory and Stress Oxidative Markers in the Antidepressant-like Activity of Aloysia citriodora and Verbascoside on Mice with Bacterial Lipopolysaccharide- (LPS-) Induced Depression

Gomes

Serpa

Miorando

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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Aloysia citriodora Palau is popularly used to treat nervous disorders. Experimental evidence has indicated that verbascoside (VBS) isolated from A. citriodora has pharmacological potential. In this study, we evaluated the antidepressant-like effects of a hydroalcoholic extract of A. citriodora (HEAc) and VBS against lipopolysaccharide- (LPS-) induced depressive-like behavior in mice. In the pretreatment protocol (performed to evaluate the preventive potential), mice were pretreated with HEAc (3, 30, or 300 mg/kg) or VBS (30 mg/kg) before the administration of LPS. In the posttreatment protocol (performed to evaluate the therapeutic potential), mice were initially administered LPS and were subsequently given HEAc (3, 30, or 300 mg/kg) or VBS (30 mg/kg). In both treatments, the mice were submitted to an open-field test and tail suspension test (TST) at 6 and 24 h after LPS administration. The posttreatment evaluation revealed that HEAc (30 or 300 mg/kg) and VBS produced an antidepressant-like effect, as indicated by a reduction in the time spent with no movement in the TST. Moreover, HEAc (30 or 300 mg/kg) was found to reduce interleukin-6 (IL-6) levels and N-acetyl-glycosaminidase activity in the hippocampus, increase glutathione (GSH) levels in the hippocampus and cortex, and enhance IL-10 in the cortex and, at a dose of 300 mg/kg, reduced myeloperoxidase activity in the cortex. Contrastingly, no comparable effects were detected in mice subjected to the pretreatment protocol. Administration of VBS similarly reduced the levels of IL-6 in the hippocampus and increased GSH levels in the cortex. Our observations indicate that both HEAc and VBS show promising antidepressant-like potential, which could be attributed to their beneficial effects in reducing neuroinflammatory processes and antioxidant effects in the central nervous system.

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Section: Discussionsupporting

confidence: 89%

Involvement of Anti-Inflammatory and Stress Oxidative Markers in the Antidepressant-like Activity of Aloysia citriodora and Verbascoside on Mice with Bacterial Lipopolysaccharide- (LPS-) Induced Depression

Gomes

Serpa

Miorando

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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“…The results were expressed as mg of gallic acid equivalents per g of dry matter (mg EGA/g DM) (Yeddes et al, 2022). The estimation of the total flavonoid content in different extracts was carried out by the method of Tammar et al (2021). The concentration of the flavonoids was calculated by referring to a calibration curve obtained using catechin as standard.…”

Section: Phenolic Compound Analysismentioning

confidence: 99%

Optimization of antioxidant and lycopene extraction from tomato pomace using Hansen solubility parameters and its application in chicken meat preservation

Karoui,

Chaabani,

Dali

et al. 2023

Journal of Food Science

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Tomato pomace, composed of peels and seeds, is often discarded or used as animal feed. However, it contains valuable phytochemicals, including lycopene. Lycopene, a natural pigment, is an antioxidant known for reducing the risk of chronic diseases like cardiovascular ailments and cancer. In this study, we aimed to study the possibility of valorizing tomato pomace by quantifying phenolic compounds, evaluating the antioxidant activity of their extracts, as well as extracting and quantifying lycopene, and studying the effect of tomato peel extract on the oxidative stability of chicken patties during storage. The effectiveness of different solvent mixtures for the extraction of lycopene was evaluated using Hansen solubility parameters (HSPs). The obtained results showed that the best solvent mixture was hexane/acetone (50/50) with a Hansen theoretical distance of 7.2, indicating its favorable solvation power. It also achieved a notable extraction yield of 3.12% and the highest lycopene yield of 20.05 mg/100 g. This combination demonstrated the highest values in terms of total phenolic (24.06 mg equivalent gallic acid/100 g dry matter) and flavonoid content (30.55 mg equivalent catechin/100 g dry matter), indicating a significant presence of these compounds. However, its 1,1‐diphenyl‐2‐picrylhydrazyl (13.51 µg/mL) and ABTS, 2,2'‐azino‐bis(3‐ethylbenzothiazoline‐6‐sulfonic acid, (8.52 µg/mL) IC50 values were comparatively lower than the other mixes. The use of this fraction as a food additive and antioxidant showed significant competitiveness with the conventional preservative, 2,6‐di‐tert‐butyl‐4‐methylphenol. Tomato extract can be considered a potential natural preservative in food preparations due to its high lycopene content.Practical ApplicationThis research provides valuable insights into optimizing the extraction of antioxidants from tomato pomace, using HSPs. The findings have the potential to benefit the food industry by developing improved methods for preserving chicken meat through the application of these optimized antioxidant extracts. By enhancing the preservation process, this study may contribute to extending the shelf life and maintaining the quality of chicken meat, leading to reduced food waste and improved consumer satisfaction.

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“…For lemon verbena, the TPC values of the present work are in agreement with the findings of Hematian Sourki et al [ 47 ], who studied the chemical composition of alcoholic extracts obtained from Iranian lemon verbena leaves (49.2 mg GAE/g DW), while the TF values were higher in our study (14.5 mg QE/g DW). In contrast, Tammar et al [ 65 ] reported lower amounts of TPCs (from 11.66 to 29.16 mg GAE/g DW) and higher quantities of TFs (from 27.53 to 39.86 mg caffeic acid/g DW) in methanolic extracts obtained from the aerial parts of Tunisian germplasm of lemon verbena. According to Aldeen et al [ 66 ], the TPC and TF content of lemon verbena leaves may vary depending on the plant growth stage, with values ranging between 22.83 and 48.21 and 2.41 and 7.56 mg QE/g for TPCs and TFs, respectively.…”

Section: Resultsmentioning

confidence: 95%

“…For example, Kubica et al [ 17 ] analyzed methanolic extracts of Polish common verbena and found DPPH values of 0.214 mg/mL (IC 50 ) while in decoctions of common verbena, the DPPH values obtained by Babili [ 75 ] were 15.76 mg/mL (IC 50 ). Moreover, the infusions prepared from Portuguese lemon verbena leaves recorded DPPH values of 0.25 mg/mL (IC 50 ) [ 67 ] while the DPPH and ABTS values recorded in methanolic extracts of lemon verbena aerial parts from Tunisia were in the range of 12.71 to 14.90 and 4.54 to 8.10 µg/mL (IC 50 ), respectively [ 65 ]. Plaza et al [ 60 ] suggested that the temperature conditions of supercritical extraction may significantly affect the antioxidant activity of common verbena leaf extracts, with higher values being observed at 200 °C compared to 100 °C (2.156 and 0.959 mmol TE/g dw, respectively).…”

Section: Resultsmentioning

confidence: 99%

Chemical Composition and Antioxidant Properties of Common and Lemon Verbena

et al. 2022

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The nutritional profiles of common and lemon verbena leaves were analyzed (proximate constituents, free sugars, organic acids, tocopherols, and fatty acids) and the leaves were prepared in hydromethanolic and aqueous (decoctions and infusions) extracts. The phenolic compound composition and antioxidant activity (2,2-Diphenyl-1-picrylhydrazyl (DPPH); 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), ferric-reducing antioxidant power (FRAP); and cupric-reducing antioxidant capacity (CUPRAC) assays) of the extracts were characterized. The nutritional composition varied between the studied species, with lemon verbena showing higher amounts of protein, ash, and fat than common verbena, whereas the opposite trend was recorded for the dietary fiber content. The main free sugars detected in both species were fructose, glucose, and sucrose, which were present in higher amounts in the common verbena samples. Succinic acid was the most abundant organic acid in both species while high amounts of oxalic acid were detected in lemon verbena. The main fatty acids in both species were α-linolenic, palmitic, and linoleic acid. Regarding the phenolic compound content, the extracts of lemon verbena presented higher amounts of total phenolic compounds (TPCs), total flavonoids (TFs) and total phenolic acids (TPAs) than the common verbena extracts while the aqueous extracts (infusions and decoctions) were richer in TPCs, TFs, and TPAs than the hydromethanolic ones in both species. Nine phenolic compounds were identified and quantified, including seven phenolic acids and two flavonoids. The lemon verbena samples were characterized by higher antioxidant activity compared to the common verbena samples while the aqueous extracts showed higher antioxidant efficacy than the hydromethanolic ones. In conclusion, both species showed promising results in terms of the nutritional value, chemical composition, and antioxidant activities, which were positively correlated with the phenolic compound contents. Moreover, the extraction protocol may affect the chemical composition and bioactive properties of both species, with aqueous extracts showing better results than hydromethanolic ones.

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Chemometric Profiling and Bioactivity of Verbena (Aloysia citrodora) Methanolic Extract from Four Localities in Tunisia

Cited by 13 publications

References 53 publications

Involvement of Anti-Inflammatory and Stress Oxidative Markers in the Antidepressant-like Activity of Aloysia citriodora and Verbascoside on Mice with Bacterial Lipopolysaccharide- (LPS-) Induced Depression

Involvement of Anti-Inflammatory and Stress Oxidative Markers in the Antidepressant-like Activity of Aloysia citriodora and Verbascoside on Mice with Bacterial Lipopolysaccharide- (LPS-) Induced Depression

Optimization of antioxidant and lycopene extraction from tomato pomace using Hansen solubility parameters and its application in chicken meat preservation

Chemical Composition and Antioxidant Properties of Common and Lemon Verbena

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