Cullin 7 and Fbxw 8 expression in trophoblastic cells is regulated via oxygen tension: implications for intrauterine growth restriction?

Abstract

Fbxw8 and Cul7 expression reveals a complex regulation in trophoblastic cells. Our findings suggest that dysregulation of Cul7 and Fbxw8 expression might affect trophoblast turnover in IUGR.

Expression and Regulation of Retinoic Acid Receptor Responders in the Human Placenta

“…The clinical data of all patients are summarized in Supplementary Table 1. The inclusion criteria for PE and IUGR were described earlier by Fahlbusch and Ruebner et al 40,41 Placental tissues were formalin-fixed and paraffin-embedded (FFPE) for histological staining or snap frozen in liquid nitrogen for RNA extraction within 1 hour after delivery.…”

The placental mTOR-pathway: correlation with early growth trajectories following intrauterine growth restriction?

“…Seven newborns with IUGR and seven healthy gestational age-matched newborns [(appropriate for gestational age (AGA)] were enrolled in this study. IUGR was defined by a pathologic flow impedance of the umbilical artery and/or uterine arteries as shown by Doppler sonography 28 in combination with the inclusion/exclusion criteria described before 29 (Supplementary Table 1). The IUGR cases studied are considered to be idiopathic, 30 with utero-placental insufficiency as the major cause.…”

Integrin α8 Is Abundant in Human, Rat, and Mouse Trophoblasts

“…In a third set of experiments, primary trophoblast cells were isolated from uncomplicated term pregnancies after elective cesarean section as previously described in detail. 36,39 We isolated decidual stroma cells (DSCs), EVTs, and villous cytotrophoblasts (VT) from 3 total AGA placentas. The procedures were carried out under sterile conditions.…”

“…Protocols for immunomagnetic bead separation of VT and EVT isolation have been described in detail elsewhere. 36,40 In short, for the fractionation of EVT, placental basal plate was grossly removed, minced, and subjected to enzymatic digestion involving trypsin, DNase I (Sigma-Aldrich, Taufkirchen, Germany), and dispase (BD Biosciences, Heidelberg, Germany). After filtration and centrifugation, the resuspended cells were layered over lymphocyte separation medium 1077(Sigma-Aldrich).…”

“…Placental sampling and tissue handling were described earlier in detail. 36,37 For immunohistochemical analysis, fresh samples of human placenta were excised immediately after placental delivery, fixed in 4% formaldehyde, and embedded in paraffin. We analyzed tissue sections of healthy human appropriate for gestational age (AGA) placentas (n ¼ 5) and of pregnancies complicated by IUGR, PE, and HELLP ranging from 36 gestational weeks to term (n ¼ 5 each).…”