Cultivar, irrigation, and soil contribution to the enhancement of Verticillium wilt disease in cotton

Land, Caroline; Lawrence, Kathy; Burmester, C. H.; Meyer, B.

doi:10.1016/j.cropro.2017.01.002

Cited by 17 publications

(7 citation statements)

References 9 publications

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“…In cotton, the disease infection is seen at relatively early stages of plant development, though the symptoms become more severe after flowering. On the cotton plants, the symptoms are necrotic lesion on the leaf, stunted growth, premature defoliation, discoloration of the vascular system, ball abortion, and even plant death [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

RNA-Sequencing, Physiological and RNAi Analyses Provide Insights into the Response Mechanism of the ABC-Mediated Resistance to Verticillium dahliae Infection in Cotton

Dong

Magwanga

Cai

et al. 2019

Genes

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Verticillium wilt that is caused by Verticillium dahliae, does result in massive annual yield losses and fiber quality decline in cotton. Control by conventional mechanisms is not possible due to a wide host range and the longevity of dormant fungi in the soil in the case of absence of a suitable host. Plants have developed various mechanisms to boost their immunity against various diseases, and one is through the induction of various genes. In this research, we carried out RNA sequencing and then identified the members of the adenosine triphosphate (ATP)-binding cassette (ABC) proteins to be critical in enhancing resistance to V. dahliae infection. A total of 166 proteins that are encoded by the ABC genes were identified in Gossypium raimondii with varying physiochemical properties. A novel ABC gene, Gorai.007G244600 (ABCF5), was found to be highly upregulated, and its homolog in the tetraploid cotton Gh_D11G3432 (ABCF5), was then silenced through virus induced gene silencing (VIGS) in G. hirsutum, tetraploid upland cotton. The mutant cotton seedlings ability to tolerate V. dahliae infection was significantly reduced. Based on the evaluation of oxidant enzymes, hydrogen peroxide (H2O2) and malondialdehyde (MDA) showed significantly increased levels in the leaves of the mutant compared to the wild type. In addition, antioxidant enzymes, peroxidase (POD), catalase (CAT), and superoxide dismutase (SOD) concentrations were reduced in the mutant cotton leaves after treatment with V. dahliae fungi as compared to the wild type. Moreover, expression levels of the biotic stress genes, cotton polyamine oxidase (GhPAO), cotton ribosomal protein L18 (GhRPL18), and cotton polygalacturonase-inhibiting protein-1 (GhPGIP1), were all downregulated in the mutant but they were highly upregulated in the various tissues of the wild cotton seedlings. This research has shown that ABC genes could play an important role in enhancing the immunity of cotton to V. dahliae infection, and thus can be explored in developing more resilient cotton genotypes with improved resistance to V. dahliae infection in cotton.

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Section: Introductionmentioning

confidence: 99%

RNA-Sequencing, Physiological and RNAi Analyses Provide Insights into the Response Mechanism of the ABC-Mediated Resistance to Verticillium dahliae Infection in Cotton

Dong

Magwanga

Cai

et al. 2019

Genes

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“…Subsequently, the 30‐day sunflower seedlings were also spraying with DMs at the same concentration. The wilt degree was surveyed at 70‐days post‐sowing, and the disease index was calculated following the description of Land et al (2017).…”

Section: Methodsmentioning

confidence: 99%

Analysis of differential metabolites of sunflower induced by virulent Verticillium dahlia V33 and hypovirulent Gibellulopsis nigrescens Vn‐1

Peng

Dong

Wang

et al. 2022

Journal of Phytopathology

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Verticillium wilt is a widespread soil‐borne vascular disease of crops. Hypovirulent strains of pathogens show potential in providing cross‐protection against Verticillium wilt. To identify metabolites associated with the induction of resistance, gas chromatography–mass spectrometry‐based metabolomic technology was used to screen for differential metabolites induced by virulent (Verticillium dahlia V33) and hypovirulent (Gibellulopsis nigrescens Vn‐1) pathogen strains in sunflower (Helianthus annuus) roots. Forty‐eight differential metabolites were detected in the comparison between Vn‐1 and V33 inoculation treatments. Among these differential metabolites, the accumulation of 17 secondary metabolites was significantly higher in response to Vn‐1 inoculation compared with that after V33 inoculation. We selected nine differential metabolites for further analyses. Pyruvate, phenethylamine and 2‐phenylbutyric acid inhibited infection of Nicotiana benthamiana leaves by Botrytis cinerea and Phythophthora infestans. Pyruvate, phenethylamine and 2‐phenylbutyric acid induced H2O2 burst at 24 hours post‐spray (hps) treatment of N. benthamiana leaves with these organic acids. Quantitative RT‐PCR analysis revealed that pyruvate, phenethylamine and 2‐phenylbutyric acid significantly enhanced the expression of pathogenesis‐related genes at 24 hps, including PR1, NPR1 and PDF1.2. In addition, the activities of peroxidase, catalase, polyphenol oxidase and superoxide dismutase were increased. These results indicated that many metabolites were differentially induced by hypovirulent and virulent pathogen strains and were involved in resistance of sunflower against Verticillium wilt. Pyruvate, 2‐phenylbutyric acid and phenethylamine show potential for induction of plant resistance to pathogen infection.

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“…Verticillium dahliae is a soilborne phytopathogen and commonly associated with vascular wilt diseases of up to 400 host plant species; many of these are of economic importance in agriculture, horticulture and forestry [ 12 ]. V. dahliae was reported across many cotton growing regions such as Australia, China, Spain and the USA [ 8 , 13 , 14 , 15 ]. Virulence of V. dahliae was commonly found associated with its pathotypes, that being defoliating (D) and non-defoliating (ND), based on symptoms induced on host plants [ 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

Co-Occurrence of Defoliating and Non-Defoliating Pathotypes of Verticillium Dahliae in Field-Grown Cotton Plants in New South Wales, Australia

Gregson

Thao

et al. 2020

Plants

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Verticillium wilt (VW) is a major constraint to cotton production in Australia and worldwide. The disease is caused by a soilborne fungus, Verticillium dahliae, a highly virulent pathogen on cotton. Commonly, V. dahliae is designated into two pathotypes: defoliating (D) and non-defoliating (ND), based on induced symptoms. In the previous two survey seasons between 2017 and 2019, stems with suspected VW were sampled for the confirmation of presence and distribution of D and ND pathotypes across New South Wales (NSW), Australia. A total of 151 and 84 VW-suspected stems sampled from the 2017/18 and 2018/19 seasons, respectively, were subjected to pathogen isolation. Of these, 94 and 57 stems were positive for V. dahliae; and 18 and 20 stems sampled respectively from the two seasons yielded the D pathotype isolates. Two stems from the 2017/18 season and one stem from 2018/19 season yielded both D and ND pathotype isolates. We also successfully demonstrated the co-infection of both pathotypes in pot trials, which was driven predominantly by either of the pathotypes, and appeared independent on vegetative growth, fecundity and spore germination traits. Our study is the first report of the natural co-occurrence of both D and ND pathotypes in same field-grown cotton plants in NSW, to which a challenge to the disease management will be discussed.

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Cultivar, irrigation, and soil contribution to the enhancement of Verticillium wilt disease in cotton

Cited by 17 publications

References 9 publications

RNA-Sequencing, Physiological and RNAi Analyses Provide Insights into the Response Mechanism of the ABC-Mediated Resistance to Verticillium dahliae Infection in Cotton

RNA-Sequencing, Physiological and RNAi Analyses Provide Insights into the Response Mechanism of the ABC-Mediated Resistance to Verticillium dahliae Infection in Cotton

Analysis of differential metabolites of sunflower induced by virulent Verticillium dahlia V33 and hypovirulent Gibellulopsis nigrescens Vn‐1

Co-Occurrence of Defoliating and Non-Defoliating Pathotypes of Verticillium Dahliae in Field-Grown Cotton Plants in New South Wales, Australia

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