Cultivated anaerobic acidophilic/acidotolerant thermophiles from terrestrial and deep-sea hydrothermal habitats

Prokofeva, M. I.; Kublanov, Ilya V.; Nercessian, Olivier; Tourova, T. P.; Kolganova, T. V.; Lebedinsky, Alexander V.; Bonch-Osmolovskaya, E. A.; Spring, Stefan; Jeanthon, Christian

doi:10.1007/s00792-005-0461-4

Cited by 44 publications

(27 citation statements)

References 43 publications

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“…The complete genome sequence of V. distributa DSM 14429 was determined recently (7). Strain 768-28 was isolated from the solfataric field close to Moutnovsky volcano in Kamchatka, Russia, and was found to be affiliated with the Vulcanisaeta genus based on 16S rRNA gene phylogeny (8). Vulcanisaeta sp.…”

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“…768-28 is an obligately anaerobic acidophile with a pH range of growth from 3.5 to 6.5 and a temperature range from 60 to 98°C. It is a metabolically versatile archaeon capable of fermenting proteinaceous substrates and some sugars (8). Elemental sulfur and thiosulfate are reduced to hydrogen sulfide if added (8).…”

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“…It is a metabolically versatile archaeon capable of fermenting proteinaceous substrates and some sugars (8). Elemental sulfur and thiosulfate are reduced to hydrogen sulfide if added (8). To understand the physiological properties of this archaeon and possible ecological roles of Vulcanisaeta sp., a complete genome sequence was determined.…”

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Complete Genome Sequence of “Vulcanisaeta moutnovskia” Strain 768-28, a Novel Member of the Hyperthermophilic Crenarchaeal Genus Vulcanisaeta

et al. 2011

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Strain 768-28 was isolated from a hot spring in Kamchatka, Russia, and represents a novel member of the Vulcanisaeta genus. The complete genome sequence of this thermoacidophilic anaerobic crenarchaeon reveals genes for protein and carbohydrate-active enzymes, the Embden-Meyerhof and Entner-Doudoroff pathways for glucose metabolism, the tricarboxylic acid cycle, beta-oxidation of fatty acids, and sulfate reduction.

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Complete Genome Sequence of “Vulcanisaeta moutnovskia” Strain 768-28, a Novel Member of the Hyperthermophilic Crenarchaeal Genus Vulcanisaeta

et al. 2011

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“…Kamchatka hot springs for a long time served as a source of novel thermophilic microorganisms (6), including several isolates of organotrophic anaerobic Crenarchaeota (4,5,39,40,41). The diversity of nonthermophilic crenarchaeotes in soils from permafrost and grassland areas of Kamchatka studied by cultureindependent methods was reported (36).…”

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Distribution of Crenarchaeota Representatives in Terrestrial Hot Springs of Russia and Iceland

Perevalova

Kolganova

Birkeland

et al. 2008

Appl Environ Microbiol

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Culture-independent (PCR with Crenarchaeota-specific primers and subsequent denaturing gradient gel electrophoresis) and culture-dependent approaches were used to study the diversity of Crenarchaeota in terrestrial hot springs of the Kamchatka Peninsula and the Lake Baikal region (Russia) and of Iceland. Among the phylotypes detected there were relatives of both cultured (mainly hyperthermophilic) and uncultured Crenarchaeota. It was found that there is a large and diverse group of uncultured Crenarchaeota that inhabit terrestrial hot springs with moderate temperatures (55 to 70°C). Two of the lineages of this group were given phenotypic characterization, one as a result of cultivation in an enrichment culture and another one after isolation of a pure culture, "Fervidococcus fontis," which proved to be a moderately thermophilic, neutrophilic (optimum pH of 6.0 to 7.5), anaerobic organotroph.

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“…It was reported that Crenarchaeota inhabited not only high-temperature springs (Wemheuer et al, 2013;Chernyh et al, 2015), but also springs with moderate temperatures (Perevalova et al, 2008;Burgess et al, 2012;Menzel et al, 2015). At the time of writing, many thermophilic and hyperthermophilic crenarchaeotes have been isolated from hot springs of Uzon Caldera, including members of genera Desulfurococcus (Bonch- Osmolovskaya et al, 1988;Perevalova et al, 2005;Kublanov et al, 2009), Thermoproteus (Bonch- Osmolovskaya et al, 1990), Acidilobus (Prokofeva et al, 2000;2009), Vulcanisaeta (Prokofeva et al, 2005), Fervidicoccus (Perevalova et al, 2010) and Pyrobaculum (Slobodkina et al, 2015). Here we report the isolation of strain 1633 T obtained from a hot spring of the Uzon Caldera, and found to be closely related to 'Thermogladius shockii', a species with a name that was effectively, but never validly, published (Osburn & Amend, 2011 (Kevbrin & Zavarzin, 1992) and vitamin solution (Wolin et al, 1963); 0.5 g NaHCO 3 , 0.5 g Na 2 S .…”

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Thermogladius calderae gen. nov., sp. nov., an anaerobic, hyperthermophilic crenarchaeote from a Kamchatka hot spring

Kochetkova¹,

Kublanov²,

Toshchakov

et al. 2016

International Journal of Systematic and Evolutionary Microbiology

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An obligately anaerobic, hyperthermophilic, organoheterotrophic archaeon, strain 1633 T , was isolated from a terrestrial hot spring of the Uzon Caldera (Kamchatka Peninsula, Russia). Cells were regular cocci, 0.5-0.9 mm in diameter, with one flagellum. The temperature range for growth was 80-95 8C, with an optimum at 84 8C. Strain 1633 T grew on yeast extract, beef extract, peptone, cellulose and cellobiose. No growth was detected on other sugars or carbohydrates, organic acids, or under autotrophic conditions. The only detected growth products were CO 2 , acetate, and H 2 . The growth rate was stimulated by elemental sulfur, which was reduced to hydrogen sulfide. The in silico-calculated G+C content of the genomic DNA of strain 1633 T was 55.64 mol%. 16S rRNA gene sequence analysis placed strain 1633 T together with the non-validly published 'Thermogladius shockii' strain WB1 in a separate genus-level cluster within the family Desulfurococcaceae. Average nucleotide identity (ANI) results revealed 75.72 % identity between strain 1633 T and 'Thermogladius shockii' WB1. Based on these results we propose a novel genus and species with the name Thermogladius calderae gen. nov., sp. nov. The type strain of the type species is 1633 T (5DSM 22663 T 5VKM B-2946 T ).The vast majority of cultured Crenarchaeota have been isolated from terrestrial geothermal environments (Huber & Stetter, 2006). Located in Far East of Russia, Kamchatka Peninsula is a volcanically active area with hundreds of associated volcanoes and areas with geothermal activity, among which Uzon Caldera is the largest. Molecular studies have documented the presence of a significant number of cultured and uncultured Crenarchaeota in Uzon Caldera thermal pools. It was reported that Crenarchaeota inhabited not only high-temperature springs (Wemheuer et al., 2013;Chernyh et al., 2015), but also springs with moderate temperatures (Perevalova et al., 2008;Burgess et al., 2012;Menzel et al., 2015). At the time of writing, many thermophilic and hyperthermophilic crenarchaeotes have been isolated from hot springs of Uzon Caldera, including members of genera Desulfurococcus Slobodkina et al., 2015). Here we report the isolation of strain 1633 T obtained from a hot spring of the Uzon Caldera, and found to be closely related to 'Thermogladius shockii', a species with a name that was effectively, but never validly, published (Osburn & Amend, 2011).Strain 1633 T was isolated from a sample of mixed water and black mud from a freshwater hot spring (548 30.0479 N 1598 56.8519 E) located in the West Thermal Field of Uzon Caldera. The in situ water temperature and Abbreviations: ANI, average nucleotide identity; GDGT, glycerol dibiphytanyl glycerol tetraether.The GenBank/EMBL/DDBJ accession number for the complete genome sequence of Thermogladius calderae 1633 T is CP003531. The genome sequencing project of 'Thermogladius shockii' WB1 is available in the GenBank/EMBL/DDBJ databases under accession number PRJEB12686.

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Cultivated anaerobic acidophilic/acidotolerant thermophiles from terrestrial and deep-sea hydrothermal habitats

Cited by 44 publications

References 43 publications

Complete Genome Sequence of “Vulcanisaeta moutnovskia” Strain 768-28, a Novel Member of the Hyperthermophilic Crenarchaeal Genus Vulcanisaeta

Complete Genome Sequence of “Vulcanisaeta moutnovskia” Strain 768-28, a Novel Member of the Hyperthermophilic Crenarchaeal Genus Vulcanisaeta

Distribution of Crenarchaeota Representatives in Terrestrial Hot Springs of Russia and Iceland

Thermogladius calderae gen. nov., sp. nov., an anaerobic, hyperthermophilic crenarchaeote from a Kamchatka hot spring

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