Cultivation in vitro of cells derived from a human osteosarcoma

McAllister, Robert M.; Gardner, Murray B.; Greene, A.E.; C, Bradt; Nichols, Warren W.; Landing, Benjamin H.

doi:10.1002/1097-0142(197102)27:2<397::aid-cncr2820270224>3.0.co;2-x

Cited by 190 publications

(75 citation statements)

References 7 publications

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“…The human cell lines used in this study are listed in Table I (7)(8)(9)(10)(11)(12)(13). All Hs-prefixed cell lines were isolated by the Owens differential trypsinization technique (14).…”

Section: Methodsmentioning

confidence: 99%

Tumor-specific cell-mediated immunity in household contacts of cancer patients..

Byers¹,

Levin²,

Hackett³

et al. 1975

J. Clin. Invest.

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A B S T R A C T Patients with osteogenic sarcoma (and related tumors), hypernephroma, and breast carcinoma, and their household contacts were tested for tumorspecific cell-mediated immunity against these tumors with the use of a short-term chromium-51 release assay. This assay, reproducible over many months and well-correlated with the clinical course of the patients, was used to demonstrate that household contacts of patients with osteogenic sarcoma and breast carcinoma have specific immunity against the tumor type with which they have been in contact. In both types of tumors, the range of cytotoxicity values produced by lymphocytes from the household contacts was significantly higher than that of the normal population. The incidence of immunity was much higher in household contacts of patients with breast carcinoma than in those of patients with osteogenic sarcoma. Immunity was found with equal frequency in men and women, as well as in genetically and nongenetically related household contacts (guardians, adopted children, spouses).

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“…The human cell lines used in this study are listed in Table I (7)(8)(9)(10)(11)(12)(13). All Hs-prefixed cell lines were isolated by the Owens differential trypsinization technique (14).…”

Section: Methodsmentioning

confidence: 99%

Tumor-specific cell-mediated immunity in household contacts of cancer patients..

Byers¹,

Levin²,

Hackett³

et al. 1975

J. Clin. Invest.

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“…The other is the maturation of osteoblast progenitors into osteoblasts which express the various phenotypes of bone-forming ceils; production of a large amount of extracellular matrix proteins including type I collagen and osteocalcin, high levels of alkaline phosphatase (ALP) ~ activity, and responsiveness to calcitropic hormones such as parathyroid hormone (PTH) and lc~,25-dihydroxyvitamin D3 (for reviews see Rodan and Rodan, 1984;Wlodarski, 1990). The regulatory mechanism of osteoblast differentiation at the maturation stage has been extensively studied using several osteoblast-like cell lines isolated from bone and bone marrow (McAllister et al, 1971;Heremans et al, 1978;Majeska et al, 1980;Sudo et al, 1983;Partridge et al, 1983;Benayahu et al, 1989). However, the mechanism of the commitment to osteoblast differentiation is not fully understood because of a limited number of suitable in vitro model systems reflecting the commitment of osteoblast differentiation from non-osteogenic cells.…”

mentioning

confidence: 98%

Bone morphogenetic protein-2 converts the differentiation pathway of C2C12 myoblasts into the osteoblast lineage [published erratum appears in J Cell Biol 1995 Feb;128(4):following 713]

Katagiri

Yamaguchi

Komaki

et al. 1994

The Journal of Cell Biology

1,377

983

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Abstract. The implantation of bone morphogenetic protein (BMP) into muscular tissues induces ectopic bone formation at the site of implantation. To investigate the mechanism underlying this process, we examined whether recombinant bone morphogenetic protein-2 (BMP-2) converts the differentiation pathway of the clonal myoblastic cell line, C2C12, into that of osteoblast lineage. Incubating the cells with 300 ng/ml of BMP-2 for 6 d almost completely inhibited the formation of the multinucleated myotubes expressing troponin T and myosin heavy chain, and induced the appearance of numerous alkaline phosphatase (ALP)-positive cells. BMP-2 dose dependently induced ALP activity, parathyroid hormone (PTH)-dependent 3',5'-cAMP production, and osteocalcin production at concentrations above 100 ng/ml. The concentration of BMP-2 required to induce these osteoblastic phenotypes was the same as that required to almost completely inhibit myotube formation. Incubating primary muscle cells with 300 ng/ml of BMP-2 for 6 d also inhibited myotube formation, whereas induced ALP activity and osteocalcin production. Incubation with 300 ng/ml of BMP-2 suppressed the expression of mRNA for muscle creatine kinase within 6 h, whereas it induced mRNA expression for ALP, PTH/PTH-related protein (PTHrP) receptors, and osteocalcin within 24--48 h. BMP-2 completely inhibited the expression of myogenin mRNA by day 3. By day 3, BMP-2 also inhibited the expression of MyoD mRNA, but it was transiently stimulated 12 h after exposure to BMP-2. Expression of Id-1 mRNA was greatly stimulated by BMP-2. When C2C12 cells pretreated with BMP-2 for 6 d were transferred to a colony assay system in the absence of BMP-2, more than 84 % of the colonies generated became troponin T-positive and ALP activity disappeared. TGF-/31 also inhibited myotube formation in C2C12 cells, and suppressed the expression of myogenin and MyoD mRNAs without inducing that of Id-1 mRNA. However, no osteoblastic phenotype was induced by TGF-/31 in C2C12 cells. TGF-/31 potentiated the inhibitory effect of BMP-2 on myotube formation, whereas TGF-/31 reduced ALP activity and osteocalcin production induced by BMP-2 in C2C12 cells. These results indicate that BMP-2 specifically converts the differentiation pathway of C2C12 myoblasts into that of osteoblast lineage cells, but that the conversion is not heritable.S EVERAL lines of evidence indicate that osteoblasts, chondrocytes, myocytes, and adipocytes are all derived from a common progenitor cell called undifferentiated mesenchymal cells (Taylor and Jones, 1979; Grigoriadis et al., 1988Grigoriadis et al., , 1990 Yamaguchi and Kahn, 1991). During the process of their differentiation, progenitor cells acquire specific phenotypes depending upon the differentiated cell types under the control of respective regulatory factors (for reviews see Rodan and Rodan, 1984;Owen, 1988; Wlodar-Address all correspondence to Dr. Tatsuo Suda, Department of Biochemistry, School of Dentistry, Showa University, 1-5-8 Hatanodai, Tokyo 142, ski, 1990). The di...

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“…The cell line is apparently uncontaminated with HeLa cells. Although many types of human sarcoma cells have been described (Aaronson et al, 1970;Giraldo et al, 1971;loachim, 1970;McAllister et al, 1971;McAllister et al, 1975;Morton et al, 1969;Ponten and Saksela, 1967;Rasheed et al, 1974;Stewart et al, 1972;Winters et al, 1974), the establishment of continuous cell lines from human skeletal tumours has only been reported infrequently (Giraldo et al, 1971;McAllister et al, 1971;Ponten and Saksela, 1967;Winters et al, 1974 The cell line described in the present report was included in a study on the presence of oncornaviral antigens in human tumour cells (Zurcher et al, 1975). In Fig.…”

Section: Discussionmentioning

confidence: 99%

“…MORPHOLOGICAL and growth characteristics have been described for the cultured cells of a variety of human mesenchymal tumours (Aaronson, Todaro and Freeman, 1970;Giraldo et al, 1971;Joachim, 1970;McAllister et al, 1971;McAllister et al, 1975;Morton, Hall and Malmgren, 1969;Ponten and Saksela, 1967;Rasheed et al, 1974;Stewart et al, 1972a, b). Winters, Neri and Morton (1974) reported several aspects of a continuous culture derived from a human chondrosarcoma.…”

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confidence: 99%

A new cell line from a human chondrosarcoma

Man¹,

Snoep²,

Meij³

et al. 1977

Br J Cancer

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Summary.-Morphological and growth characteristics are described of a rapidly growing cell line with epithelioid and giant-cell characteristics derived from a chondrosarcoma in a male patient 65 years of age. This cell line is of considerable interest because in these cells cross-reacting antigens with known animal oncornaviruses are present. Biochemically, the cells contain particles with a density of 1-16 with " cores " of density 1-23 associated with a reverse-transcriptase-like enzyme and with 70S RNA.

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Cultivation in vitro of cells derived from a human osteosarcoma

Cited by 190 publications

References 7 publications

Tumor-specific cell-mediated immunity in household contacts of cancer patients..

Tumor-specific cell-mediated immunity in household contacts of cancer patients..

Bone morphogenetic protein-2 converts the differentiation pathway of C2C12 myoblasts into the osteoblast lineage [published erratum appears in J Cell Biol 1995 Feb;128(4):following 713]

A new cell line from a human chondrosarcoma

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